geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Genetic characterization of the mechanisms of resistance to amoxicillin/clavulanate and third-generation cephalosporins in Salmonella enterica from three Spanish hospitals


, : Genetic characterization of the mechanisms of resistance to amoxicillin/clavulanate and third-generation cephalosporins in Salmonella enterica from three Spanish hospitals. International Microbiology 14(3): 173-181

The mechanisms of antimicrobial resistance were characterized in 90 Salmonella enterica isolates either resistant or with intermediate resistance to amoxicillin/clavulanate (AMC(R/I)) or resistant to third-generation cephalosporins (C3G(R)). These isolates were recovered in three Spanish hospitals during 2007-2009. The C3G(R) phenotype was expressed by three isolates that carried the following extended-spectrum β-lactamase genes: phage-associated bla(CTX M-10) in S. Virchow, bla(CTX-M-14a) surrounded by ISEcp1 and IS903 in S. Enteritidis, and bla(CTX-M-15) linked to ISEcp1 and orf477 in S. Gnesta (first description in this serotype). The AMC(R/I) phenotype was found in 87 isolates (79 S. Typhimurim, 7 S. Enteritidis, and one S. Thompson). The bla(PSE-1) gene, followed by bla(OXA-1) was mostly found among S. Typhimurim, and the bla(TEM-1) gene among S. Enteritidis. Three different gene combinations [bla(PSE-1) +floR+aadA2+sul+tet(G); bla(OXA-1) +catA+aadA1/strA-strB+sul+tet(B) and bla(TEM-1) + cmlA1+aadA/strA-strB+sul+tet(A)/tet(B) genes] were associated with the ampicillin-chloramphenicol-streptomycin-sulfonamides-tetracycline phenotype in 68 AMC(R/I) S. enterica isolates. Class 1 integrons were observed in 79% of the isolates and in most of them (45 isolates) two integrons including the aadA2 and bla(PSE-1) gene cassettes, respectively, were detected. The bla(OXA-1) +aadA1 arrangement was detected in 23 isolates, and the aac(6')-Ib-cr+bla(OXA-1) +catB3+arr3 in another one. Non-classic class 1 integrons were found in three isolates: dfrA12+orfF+aadA2+cmlA1+aadA1 (1 isolate), dfrA12+orfF+aadA2+ cmlA1+aadA1+qacH+IS440+sul3 (1 isolate) and dfrA12+orfF+aadA2+cmlA1+aadA1+qacH+IS440+ sul3+orf1+mef(B)Δ-IS26 (1 isolate). Taken together, these results underline the need for clinical concern regarding β-lactam resistance in Salmonella and thus for continuous monitoring.

US$19.90

PMID: 22101415

DOI: 10.2436/20.1501.01.146


Other references

Varma, D.R.; Sareen, K.N.; Roy, A.K.; Gujral, M.L., 1959: Oral contraptive--Part III. Further observations on the antifertility effect of rottlerin. Indian Journal of Physiology and Pharmacology 3: 168-172

Gristina, G.R., 1998: Should monitoring standards in anesthesia defined by SIAARTI be reconsidered? Yes, but to what extent?. Minerva Anestesiologica 64(3): 103-106

Blackwood, D.H.; Visscher, P.M.; Muir, W.J., 2001: Genetic studies of bipolar affective disorder in large families. Background Genetic factors are known to be important in the aetiology of bipolar disorder. Aims To review linkage studies in extended families multiply affected with bipolar disorder. Method Selective review of linkage studies of bipolar disorder...

Martins, C.C.; Nakagawa, J.; Bovi, M.L.A., 2000: Desiccation tolerance of four seedlots from Euterpe edulis Mart. In order to identify the desiccation tolerance of E. edulis seeds, 4 fruit lots were collected from the Campinas Agronomic Institute Palmae collection, located at Ubatuba, state of Sao Paulo, Brazil, and taken in moisture-proof containers to the C...

Tague,C.; Pohl-Costello,M., 2008: The potential utility of physically based hydrologic modeling in ungauged urban streams. A wide range of hydrologic modeling tools based on geographic information systems are available to aid urban planning, design, and environmental assessment by providing an understanding of how urban development impacts water quality and water quan...

Schaffer J.L.; Davidson R.S.; Ecker M.L.; Drummond D.S., 1990: Congenital vertebral anomalies associated with pulmonary hypoplasia. Orthopaedic Transactions 14(3): 789-790

O'Sullivan, C.J.; Stefanini, G.G.; Stortecky, S.; Tüller, D.; Windecker, S.; Wenaweser, P., 2015: Coronary revascularization and TAVI: before, during, after or never?. Aortic valve stenosis and coronary artery disease (CAD) frequently coexist in elderly patients selected for transcatheter aortic valve implantation (TAVI). Therapeutic strategies to manage concomitant obstructive CAD are therefore an important con...

Melisch, Roland, 1994: Observation of swimming Babirus Babyrousa babyrussa in Lake Poso, Central Sulawesi, Indonesia. Malayan Nature Journal 47(4): 431-432

Saari, J.T.; Beck, J.S., 1975: Hypotonic hemolysis of human red blood cells a 2 phase process. Journal of Membrane Biology 23(3-4): 213-226

Hoy T.; Turner R.J.W.; Leitch C.H.B.; Anderson D., 1995: Metallogeny of the early rift phase of the Purcell Basin, SE B.C. Program with Abstracts - Geological Association of Canada(Mineralogical Association of Canada(Canadian Geophysical Union, Joint Annual Meeting 20: 46