geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Ghrelin acts on rat dorsal vagal complex to stimulate feeding via arcuate neuropeptide Y/agouti-related peptide neurons activation


, : Ghrelin acts on rat dorsal vagal complex to stimulate feeding via arcuate neuropeptide Y/agouti-related peptide neurons activation. Sheng Li Xue Bao 62(4): 357-364

Ghrelin, an endogenous ligand for the growth hormone secretagogue (GHS) receptor, stimulates feeding and increases body weight. The primary action site of ghrelin has been reported to be the neuropeptide Y (NPY)/agouti-related peptide (AgRP) neurons in the hypothalamic arcuate nucleus (ARC). In addition to the hypothalamus, the caudal brainstem also appears to be an important mediator for the orexigenic activity of ghrelin. However, it is not clear whether ghrelin applied directly to the caudal brainstem activates forebrain structures. The aim of this study was to determine whether recruitment of forebrain structures was required for hyperphagic responses stimulated by ghrelin delivery within the caudal brainstem. In our experiment, all rats were surgically implanted with indwelling cannulas in the dorsal vagal complex (DVC), and ghrelin (20 pmol in 0.5 μL) was delivered to the DVC. After the injection, the orexigenic response to ghrelin was recorded by Feeding and Activity Analyser, and NPY/AgRP mRNA expressions in rat hypothalamus were detected by real-time PCR. In addition, the NPY immunoreactive neurons in the ARC were assayed by immunohistochemistry. The results showed that ghrelin significantly increased cumulative food intake at 1, 2 and 3 h after ghrelin injection, maximal response occurring at 2 h after injection. NPY/AgRP mRNA levels in ARC treated with ghrelin increased significantly compared with those in control group (injected with saline). The highest levels of NPY and AgRP mRNA were detected at 2 h after injection. The total number and mean optical density of NPY-positive neurons increased in ghrelin treated rats compared with those in control group. Consistently, ghrelin's effect was most pronounced at 2 h after injection. Taken together, we conclude that the activation of NPY/AgRP neurons in the ARC is involved in the mediation of the hyperphagic response to brainstem ghrelin administration in neurologically intact rats.

US$29.90

PMID: 20717637


Other references

Lesniaková, M., 1975: Means and methods of rearing children under 3 years old. Zdravotnicka Pracovnice 25(11): 661-664

Giusti G.; Galanti B., 1984: Post transfusion hepatitis point of view of the specialist in infectious diseases. Ricerca in Clinica e in Laboratorio 14(SUPPL 1): 3-16

Knöbber, D.; Jahnke, V., 1991: Metastasis to the ENT area. We report three patients with metastases to the ENT-region mimicking a primary malignant tumour. A 36-year-old woman presented with vertigo, sudden hearing loss, partial facial palsy and headaches. CT scan suggested a meningioma or an acoustic neu...

Rossi, M.; Ghiara, M.R.saria; Chita, G.; Capitelli, F., 2011: Crystal-chemical and structural characterization of fluorapatites in ejecta from Somma-Vesuvius volcanic complex. The mineralogy and crystal chemistry of apatites occurring in 14 ejecta of historical eruptions (1631 and 1872 A.D.) of the Somma-Vesuvius volcanic complex were investigated by a multi-methodological approach, including polarized optical microscop...

Bek, TA., 1972: The feeding of intertidal gammarids (Amphipoda, Gammaridea). Vestnik mosk. gos. Univ, 1: 106-107

Ghizzoni, L.; Virdis, R.; Vottero, A.; Cappa, M.; Street, M.E.; Zampolli, M.; Ibañez, L.; Bernasconi, S., 1996: Pituitary-ovarian responses to leuprolide acetate testing in patients with congenital adrenal hyperplasia due to 21-hydroxylase deficiency. To assess whether patients with congenital adrenal hyperplasia (CAH) due to 21-hydroxylase deficiency exhibit a steroidogenic response to GnRH agonist consistent with functional ovarian hyperandrogenism (FOH) and elucidate the relationship between...

Granger, C.D., 1983: Storing angina pills. Lancet 2(8353): 793-793

Lamothe Argumedo, R., 1973: Monogeneans of amphibians of Mexico. IV. Redescription of Neodiplorchis scaphiopi (Rodgers, 1941) Yamaguti, 1963. Neodiplorchis scaphiopi is redescribed from specimens from Scaphiopus (Spea) hammondii, a new host, from Capulhuac State, Mexico. N. scaphiopi, the type and only species of the genus, has previously been recorded from Scaphiopus bombifrons in Okla...

Vilks G., 1968: Foraminiferal study of the Magdalen Shallows, Gulf of Saint Lawrence. Bottom conditions on the Magdalen Shallows are characterized by low water temperatures, coarse sediments, and relatively featureless submarine topography; shallower nearshore waters, with drastic seasonal changes in temperature, are also exposed t...

Epps J.M., 1995: Environmental management in mining. Australasian Institute of Mining and Metallurgy Yearbook 1995-1996: 50-51, 54