geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Glucagon Secreting Cells Responds to Insulin Secretion In vitro Using Immunocytochemistry


, : Glucagon Secreting Cells Responds to Insulin Secretion In vitro Using Immunocytochemistry. Journal of Young Pharmacists 2(2): 144-147

In the present study, pancreas of rats were dissected and transferred to HEPES buffer (25 mM, pH 7.4). The control tissue pieces were kept in culture medium for one hour and the treated tissues were kept in same medium for 30 minutes and incubated with Insulin (10 nm and 100 nm) for another half hour, then tissues were transferred to Bouin's fixative (overnight at 40 ° Cc), cryosectioned (15 µm at -16 0 c) and subjected to immunocytochemical labeling with antibodies against Glucagon. In the sections of control tissue, the Glucagon Immunoreactive Cells (GIC) were distinctly visible; on average 40-50 cells were counted in each islet. However in vitro treatment with 10 nm insulin caused 285.89 % increase in the GIC and was found to be highly significant (P< 0.001). Whereas in 100 nm Insulin treatment, 206.41% increase in GIC was seen, this was significant with the control but non-significant with 10 nm Insulin treatment.

US$19.90

PMID: 21264116

DOI: 10.4103/0975-1483.63154


Other references

Chisholm, A.H., 1948: Why do birds probe their nests ?. Victorian Naturalist, 65: 30-32

V.K.N.M.non; A.K.V.rma, 1982: Sulfate uptake in the cyanobacterium spirulina platensis. FEMS Microbiology Letters 13(2): 141-146

Abele, D.; Puntarulo, S., 2004: Formation of reactive species and induction of antioxidant defence systems in polar and temperate marine invertebrates and fish. High oxygen solubility at cold-water temperature is frequently considered to be responsible for an apparently elevated level of antioxidant protection in marine ectotherms from polar environments. However, tissue oxidative stress is in most cases...

Kurata, A.; Irikura, K.; Miyasaka, Y.; Yada, K.; Kan, S., 1992: Experience with BALT magic catheter (PURSIL catheter); especially investigation about advantage, disadvantage and the applications. BALT magic catheter (PURSIL catheter) is a new catheter which is more flexible and pliant than the TRACKER-18 catheter because its mid-section and distal portion are made from polyurethane and silicon rather than polyethylene. Because the PURSIL c...

Rupp, F.; Acha-Orbea, H.; Hengartner, H.; Zinkernagel, R.; Joho, R., 1985: Identical V beta T-cell receptor genes used in alloreactive cytotoxic and antigen plus I-A specific helper T cells. T lymphocytes involved in the cellular immune response carry cell-surface receptors responsible for antigen and self recognition. This T-cell receptor molecule is a heterodimeric protein consisting of disulphide-linked alpha- and beta-chains with...

Haçarız, Oçun.; Sayers, Góid.; Baykal, A.Tarık., 2012: A proteomic approach to investigate the distribution and abundance of surface and internal Fasciola hepatica proteins during the chronic stage of natural liver fluke infection in cattle. Fasciola hepatica, a trematode helminth, causes an economically important disease (fasciolosis) in ruminants worldwide. Proteomic analysis of the parasite provides valuable information to understand the relationship between the parasite and its ho...

Gabrieli, E.R., 1991: The changing concept of computerized patient records. Healthcare Informatics 8(6): 16-17

Monroe, J.L., 2003: Critical temperature of the Potts models on the kagomé lattice. We investigate the Wu conjecture for the critical temperature of the Potts model on the kagomé lattice. Previous testing of this conjecture has been for small values of q. Our emphasis is on large values of q where we are able to obtain very accu...

Fukuda K.; Ohshima H.; Kondo T., 1988: Interactions of poly acrylic acid complexed dipalmitoylphosphatidylcholine vesicles with surfactants. Interactions of poly(acrylic acid)-complexed dipalmitoylphosphatidylcholine vesicles with surfactants were studied by measuring the amounts of fluorescent marker, phospholipid and polymer released from the vesicles, the ESR spectrum of a spin prob...

Shol, V.A., 1960: On the natural infection of opisthorchiasis. Trudy Instituta Zoologii Alma-Ata, 14: 61-63