geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Glycopeptide-resistant enterococci carriage: Are actual isolation and identification techniques sufficient?


, : Glycopeptide-resistant enterococci carriage: Are actual isolation and identification techniques sufficient?. Pathologie-Biologie 59(3): 146-150

The monitoring of infection by glycopeptide-resistant enterococci (GRE) is one of the main elements of hospital hygiene policy. It involves systematic rectal swabs in clinics at risk (asymptomatic carriage). We compare two GRE screening methods and evaluate a new kit associating multiplex PCR and hybridization (Génotype(®) Enterococcus, Hain Lifescience) on a panel of 448 samples collected over a 4-month period. The first method is based on direct inoculation of the sample; the second one involves a preliminary enrichment phase followed by molecular diagnosis allowing the identification of species of enterococci as well as glycopeptide resistance genes. All the resistant strains were isolated using the enrichment technique. The incidence of GRE (VanA) carriage was 0,55% (two out of 362 patients, two out of 448 isolates) with two Enterococcus faecium VanA. Six Enterococcus gallinarum VanC1 and two Enterococcus casseliflavus VanC2/C3 were also isolated and identified. The main clinics concerned are intensive care and hematology. The two patients with E. faecium VanA had been previously given glycopeptides for 10 days. For three strains, the molecular method allowed to correct prior erroneous results based on rapid identification (RapidID32Strep V2.0). The method using direct samples inoculation underestimates real incidence of GRE carriage. The performances of Génotype(®) Enterococcus molecular method, evaluated for other parameters using reference strains and DNA sequencing, offer new possibilities applicable to routine laboratory.

US$19.90

PMID: 19477606

DOI: 10.1016/j.patbio.2009.04.004


Other references

Suri, A.; Lovitch, S.B.; Unanue, E.R., 2005: The wide diversity and complexity of peptides bound to class II MHC molecules. The identification and quantitation of peptides selected by class II MHC molecules during natural processing of proteins is of key importance in understanding the repertoire and distribution of T cells. The examination of peptides selected by clas...

Morrison, J.F., 1979: Approaches to kinetic studies on metal-activated enzymes. Methods in Enzymology 63: 257-294

Thomas, L., 1995: Responding to Alzheimer's. Elderly Care 7(4): 3-3

Zhang, Y.; Zeleznik-Le, N.; Emmanuel, N.; Jayathilaka, N.; Chen, J.; Strissel, P.; Strick, R.; Li, L.; Neilly, M.Beth.; Taki, T.; Hayashi, Y.; Kaneko, Y.; Schlegelberger, B.; Rowley, J.D., 2004: Characterization of genomic breakpoints in MLL and CBP in leukemia patients with t(11;16). The recurring chromosome translocation t(11;16)(q23;p13) is detected in leukemia patients, virtually all of whom have received previous chemotherapy with topoisomerase (topo) II inhibitors. In the t(11;16), 3' CBP, on 16p13, is fused to 5&apo...

Hirsch E.Z.; Maksem J.A.; Gagen D., 1984: Effects of stress and propranolol on the aortic intima of rats. Arteriosclerosis 4(5): 526A

Kim, H.Jun.; Young, J.K.; Helms, G.L.; Evans, J.N.S., 2003: 1H, 13C, and 15N backbone resonance assignments of the C-terminal domain of 5-enolpyruvylshikimate-3-phosphate synthase. Journal of Biomolecular Nmr 24(3): 269-270

Durkovic Dakovic, O.; Sibalic, D., 1986: IgM antibodies to Toxoplasma gondii in the diagnosis of congenital toxoplasmosis. An IFAT was used to detect IgM in 17 infants who had previously been diagnosed as having congenital toxoplasmosis. Results showed that the IFAT did not detect IgM in 6 of the 17 infants, although the other 11 infected infants showed high titres of...

Switzer, C.M., 1967: Response of crabgrass seedlings to various herbicides. In laboratory and glasshouse experiments, extremely low concentrations (less than 0.5 p.p.m.) of chlorthal dimethyl, bensulide, DMPA, siduron and Sindone inhibited the growth of crabgrass germinating in petri dishes. Redtop (Agrostis alba) seedlin...

Mesa, A., 1962: Cytogenetics of Dichroplus bergi. Rev Agric [Piracicaba] 37(1): 41-47

Hérouart, D.; Sigaud, S.; Moreau, S.; Frendo, P.; Touati, D.; Puppo, A., 1996: Cloning and characterization of the katA gene of Rhizobium meliloti encoding a hydrogen peroxide-inducible catalase. To investigate the involvement of bacterial catalases of the symbiotic gram-negative bacterium Rhizobium meliloti in the development of Medicago-Rhizobium functional nodules, we cloned a putative kat gene by screening a cosmid library with a catal...