+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Heterologous gene expression by chromosomal integration in fission yeast

, : Heterologous gene expression by chromosomal integration in fission yeast. Methods in Molecular Biology 824: 433-450

Thanks to the convenience and flexibility of the multicopy plasmid-based approach for heterologous gene expression, this technique has long been used for biological studies, especially in prokaryotes and lower eukaryotes. For better understanding of biological mechanisms, however, there are increasing demands on the experimental technologies enabling fine-tuned expression of introduced heterologous genes or serving conditions that are closer to the physiological conditions. For this purpose, the use of direct tagging of a chromosomal gene has been gradually increasing, although the use conditions of this approach are relatively limited compared to plasmid-based methods. Expression of a cloned gene using chromosomal integration has a property intermediate between multicopy plasmid-based method and direct tagging of an endogenous gene. Here, we describe the principle and methods of introduction of a cloned gene into the targeting loci of the chromosome in fission yeast.


PMID: 22160913

DOI: 10.1007/978-1-61779-433-9_23

Other references

Pearse, H.E., 2011: Results from using vitallium tubes in biliary surgery. Annals of Surgery 124(6): 1020-1029

Anonymous, 1947: Annual Report of the Tea Research Institute of Ceylon for the year 1947. Previously selected tea clones are now being studied for their resistance to blister blight. Tables give information on the yields and susceptibility to blister blight of several clonal series. Resistance has been observed among a number of select...

Kuo, J.M.n; Pan, B.S.n, 1991: Effects of lipoxygenase on formation of the cooked shrimp flavor compound-5,8,11-tetradecatrien-2-one. Agricultural and Biological Chemistry 55: 7-8

Flint, M.; Eden, P.A.; Limpus, C.J.; Owen, H.; Gaus, C.; Mills, P.C., 2016: Clinical and Pathological Findings in Green Turtles (Chelonia mydas) from Gladstone, Queensland: Investigations of a Stranding Epidemic. An investigation into the health of green turtles was undertaken near Gladstone, Queensland, in response to a dramatic increase in stranding numbers in the first half of 2011. A total of 56 live turtles were subject to clinical examination and blo...

Sajjan Singh; Singh, J.K.; Yadav, P.S.; Hooda, O.K., 2001: Techniques of sexing in farm animals. Invasive and non-invasive methods of determining the sex of an embryo discussed are: identification of Barr bodies in the embryo and amniotic cells; karyotyping or cytological studies; utilization of DNA probes; polymerase chain rection; detection...

Patil, G.P., 1962: On the homogeneity and combined estimation for the generalized power series distribution and certain applications. Let T be an arbitrary non-null subset of non-negative integers and define the generating function f([theta]) =[SIGMA]ax[theta]x, the summation extending over T, with ax>o; [theta] [greater than or equ so that 1([theta]) is finite and differenti...

Olmo, Jm, 1984: Noctuids del Ripolles. Especie nova per a la fauna catalana. Butlleti de la Societat Catalana de Lepidopterologia: 8 (45)

Isaeva, V.V.; Shukalyuk, A.I.; Trofimova, A.V.; Korn, O.M.; Rybakov, A.V., 2001: The structure of colonial interna in Sacculina polygenea (Crustacea: Cirripedia: Rhizocephala). Crustacean Research. December; 30: 133-146

Tryba, M., 1991: Pneumonia and continuous enteral feeding. Jpen. Journal of Parenteral and Enteral Nutrition 15(5): 582-583

Ackerman, N.; Isaza, R.; Greiner, E.; Berry, C.R., 1992: Pneumocoelom associated with Serratospiculum amaculata in a bald eagle. Serratospiculum amaculata Nematodes were identified radiographically in the air sacs of an eagle which developed acute respiratory distress several days after treatment for severe head trauma. The nematodes were found at necropsy within the air sa...