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Hydrophilic diol monolith for the preparation of immuno-sorbents at reduced nonspecific interactions

, : Hydrophilic diol monolith for the preparation of immuno-sorbents at reduced nonspecific interactions. Journal of Separation Science 34(16-17): 2097-2105

A polar organic polymer monolith (M1) was introduced for performing immunoaffinity chromatography (IAC) at reduced nonspecific interactions. The M1 monolith was prepared by the in situ polymerization of glyceryl methacrylate (GMM) and pentaerythritol triacrylate (PETA). Through its surface diol groups, M1 provided the functionalities to immobilize antibodies. Anti-haptoglobin antibody was used as the model antibody to study the overall behavior of the immuno monolith M1 in terms of its binding to the antigen and to evaluate its nonspecific binding with other proteins, especially the high-abundance human serum proteins. To better assess the suitability of M1 for IAC, other immuno monoliths were prepared and compared with the immuno monolith M1. Two monoliths were of the traditional ones: copolymers of (i) glycidyl methacrylate and ethylene glycol dimethacrylate (EDMA) and (ii) GMM and EDMA, referred to as M2 and M3, respectively. A fourth monolith involving the copolymerization of N-(3-aminopropyl)methacrylamide hydrochloride and EDMA (M4) was introduced to allow the site-directed immobilization of antibodies. Owing to its hydroxyl groups, the M1 exhibited negligible nonspecific hydrophobic interactions with proteins. On the other hand, M4 exhibited extensive electrostatic interactions, while the M2 and to a lesser extent M3 exhibited hydrophobic interactions.


PMID: 21721125

DOI: 10.1002/jssc.201100353

Other references

Gattinger T.E., 1980: Environmental geology maps; an instrument for land use and regional planning. Verhandlungen der Geologischen Bundesanstalt (Wien) 1980(3): 229-240

Ambre J.J.; Fischer L.J., 1971: The effect of coadministration of aluminum or magnesium hydroxide on the absorption of oral anti coagulants in man. Pharmacologist 13(2): 317

Phillips, A.G.; Carter, D.A.; Fibiger, H.C., 1976: Differential effects of p chlorophenyl alanine on self stimulation in caudate putamen and lateral hypothalamus. Rats were prepared with chronic bipolar electrodes aimed at either the caudate-putamen or lateral hypothalamus and those displaying consistent self-stimulation were given additional training at half-maximal current intensities. All subjects receiv...

Eskelinen de Folch, T.; Folch, P., 1988: Negative transference: from splitting to integration. Psyche 42(8): 689-708

Polansky, J.K.; Huehn, J., 2007: To be or not to be a Treg: epigenetic regulation of Foxp3 expression in regulatory T cells. Regulatory T cells (Treg) harbor great therapeutic potential for the treatment of autoimmune diseases due to their potent suppressive capacity. The majority of these cells express the transcription factor Foxp3, which is critical for both developm...

Maskarinec, G.; Takata, Y.; Kaaks, R., 2004: The relation between nutritional factors and insulin-like growth factor-I in premenopausal women of different ethnicity. Alterations in the insulin-like growth factor-I (IGF-I) system have been proposed as a metabolic link between nutritional factors and cancer risk. This study explored dietary determinants of circulating IGF-I and IGF binding protein-3 (IGFBP-3) le...

Sweeney, J.D.; Hoernig, L.A.; Behrens, A.A.; Bloom, M.L., 1992: Hand surgery in Glanzmann's thrombasthenia. American Journal of Hematology 41(2): 140-141

Mudge, G.P., 1908: On some features in the hereditary transmission of the albino character and the black piebald coat in Rats. Paper ii. Proceedings of the Royal Society London Ser B: 80 388-393

Mileman P.; Purdell Lewis D.; Van Der Weele L., 1983: Effect of variation in caries diagnosis and degree of caries on treatment decisions by dental teachers using bitewing radiographs. Nine teachers of clinical dentistry were presented with 12 paris of duplicated bitewings on 4 different occasions. At the first 2 examinations they indicated the presence of caries in need of treatment and at the second 2 the degree of caries. The...

Hayakawa, K.; Yanaihara, H., 2002: Radiation pneumonitis: Pathophysiology and treatment. Nihon Rinsho. Japanese Journal of Clinical Medicine 60 Suppl 5: 546-549