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Identification of two partners from the bacterial Kef exchanger family for the apical plasma membrane V-ATPase of Metazoa

, : Identification of two partners from the bacterial Kef exchanger family for the apical plasma membrane V-ATPase of Metazoa. Journal of Cell Science 121(Pt 15): 2612-2619

The vital task of vectorial solute transport is often energised by a plasma membrane, proton-motive V-ATPase. However, its proposed partner, an apical alkali-metal/proton exchanger, has remained elusive. Here, both FlyAtlas microarray data and in situ analyses demonstrate that the bacterial kefB and kefC (members of the CPA2 family) homologues in Drosophila, CG10806 and CG31052, respectively, are both co-expressed with V-ATPase genes in transporting epithelia. Immunocytochemistry localises endogenous CG10806 and CG31052 to the apical plasma membrane of the Malpighian (renal) tubule. YFP-tagged CG10806 and CG31052 both localise to the plasma membrane of Drosophila S2 cells, and when driven in principal cells of the Malpighian tubule, they localise specifically to the apical plasma membrane. V-ATPase-energised fluid secretion is affected by overexpression of CG10806, but not CG31052; in the former case, overexpression causes higher basal rates, but lower stimulated rates, of fluid secretion compared with parental controls. Overexpression also impacts levels of secreted Na+ and K+. Both genes rescue exchanger-deficient (nha1 nhx1) yeast, but act differently; CG10806 is driven predominantly to the plasma membrane and confers protection against excess K+, whereas CG31052 is expressed predominantly on the vacuolar membrane and protects against excess Na+. Thus, both CG10806 and CG31052 are functionally members of the CPA2 gene family, colocalise to the same apical membrane as the plasma membrane V-ATPase and show distinct ion specificities, as expected for the Wieczorek exchanger.


PMID: 18628302

DOI: 10.1242/jcs.033084

Other references

Berreen, JM., 1984: 14 Stored product mites 141 The functional response of Cheyletus eruditus Schrank to changes in the density of its prey Acarus siro L. Unknown

Matsumoto, K.; Takahashi, H.; Miyake, Y.; Fukuyama, Y., 1999: Convenient syntheses of neurotrophic americanol A and isoamericanol A by HRP catalyzed oxidative coupling of caffeic acid. Extremely simple syntheses of americanol A (1) and isoamericanol A (2) having intriguing neurotrophic properties have been accomplished by HRP catalyzed oxidative coupling of caffeic acid.

Trevaskis, J.L.; Mack, C.M.; Sun, C.; Soares, C.J.; D'Souza, L.J.; Levy, O.E.; Lewis, D.Y.; Jodka, C.M.; Tatarkiewicz, K.; Gedulin, B.; Gupta, S.; Wittmer, C.; Hanley, M.; Forood, B.; Parkes, D.G.; Ghosh, S.S., 2014: Improved glucose control and reduced body weight in rodents with dual mechanism of action peptide hybrids. Combination therapy is being increasingly used as a treatment paradigm for metabolic diseases such as diabetes and obesity. In the peptide therapeutics realm, recent work has highlighted the therapeutic potential of chimeric peptides that act on t...

Bemanian, A.; Beyer, K.M.M., 2017: Measures Matter: The Local Exposure/Isolation (LEx/Is) Metrics and Relationships between Local-Level Segregation and Breast Cancer Survival. <b>Background:</b> The Black-to-White disparity in breast cancer survival is increasing, and racial residential segregation is a potential driver for this trend. However, study findings have been mixed, and no study has comprehensively...

Liang, Y.; Chu, H.; Wang, S., 2015: Is prehydration necessary for cesarean delivery in women with Fontan repair under epidural anesthesia?. Journal of Clinical Anesthesia 27(5): 431-432

Baba, Y.; Shimozawa, T.; Kumagai, T., 1997: Distribution of GABA-immunoreactive neurons in the terminal abdominal ganglion of the cricket. Zoological Science (Tokyo) 14(SUPPL ): 106, Dec

Vuitton D.; Eloy R.; Grenier J.F., 1977: Effects of the pre treatment of the donor on the graft vs host reaction induced by intra hepatic peyers patches implantation in the rat. European Surgical Research 9(SUPPL 1): 89

Amin, S.B.; Verhaak, R.G.W., 2017: Navigating the Cancer Transcriptome by Decoding Divergent Oncogenic States. A new approach decomposes aberrant signaling mediated by an oncogenic mutation into underlying core cellular states that may be more permissive to available therapeutic options.

Saitov, M.M., 1964: The effect of time and methods of sowing on yields and the crop quality in hemp. Medium-late sowing (5-15 May) resulted in optimum yields of hemp fibre in the Tatar ASSR; the stems developed longer internodes with better quality fibre than at early sowing and the proportion of staminal plants was slightly higher.

Muscari, A.; Bozzoli, C.; Massarelli, G.; Puddu, G.M.; Palareti, G.; Legnani, C.; D'Atena, T.; Mazzuca, A.; Miniero, R.; Toscano, V., 1995: Complement components and fibrinogen: correlations and association with previous myocardial infarction. Serum complement levels have been found to be predictive of myocardial infarction up to 4 years before the acute event. To assess whether they are a marker of a hypercoagulable state, the serum or plasma levels of C3, C4, C3a, C4a, C1 inactivator,...