+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Identification, characterization, immobilization of a novel type hydrolase (LmH) from Listeria monocytogenes

, : Identification, characterization, immobilization of a novel type hydrolase (LmH) from Listeria monocytogenes. International Journal of Biological Macromolecules 72: 63-70

A novel type of hydrolase (LmH) from Listeria monocytogenes was identified, characterized, and immobilized for biotechnological applications. Primary sequence analysis indicated that LmH had a catalytic triad (Ser(91)-Asp(192)-His(222)) with a molecular weight of 27.8 kDa. Homologs of this enzyme are produced by many Gram-positive bacteria including Bacillus, Staphylococcus, and Enterococcus. Biochemical properties of LmH were investigated by performing mass spectrometry, dynamic light scattering (DLS), enzyme assays, enantioselective analysis, circular dichroism (CD) spectroscopy, fluorescence analysis, and macroscopic hydrogel formations. Interestingly, cross-linked enzyme aggregates (CLEAs) of LmH exhibited enhanced stability and good recycling abilities compared to free LmH. These molecular characteristics of LmH highlight its great potential for the pharmaceutical, biotechnological, and chemical industries.


PMID: 25128099

DOI: 10.1016/j.ijbiomac.2014.07.058

Other references

Jørgensen, B.B.; Bak, F., 1991: Pathways and microbiology of thiosulfate transformations and sulfate reduction in a marine sediment (kattegat, denmark). Reductive and oxidative pathways of the sulfur cycle was studied in a marine sediment by parallel radiotracer experiments with 35SO42-, H235S, and 35S2O32- injected into undisturbed sediment cores. The distributinos of viable populations of sulfat...

Martin H., 1963: A suggested theory for the origin and a brief description of some gypsum deposits of South West Africa. The gypsum is an alteration product of Tertiary and Pleistocene calcretes ranging from pure calcium carbonate rocks to lime-cemented sands and gravels. Alteration proceeded from the surface downward, attaining depths of three feet, with the purity...

Aversano, T.; Zhou, W.; Nedelman, M.; Nakada, M.; Weisman, H., 1995: A chimeric IgG4 monoclonal antibody directed against CD18 reduces infarct size in a primate model of myocardial ischemia and reperfusion. Objectives. This study attempted to determine whether neutrophil sequestration in reperfused myocardium can be inhibited and infarct size reduced by treatment with a chimeric, monoclonal IgG4 antibody (CLB54) directed against CD18 in a primate mod...

Duhamel G.; Salmon C.; Andre R., 1968: Hemolytic anemias with atypical lymphoid hemopathy human neopl lymphoid leukemia neopl waldenstroems disease neopl sarcoma. Revue Roumaine de Medecine Interne 5(3): 159-168

Miyoshi, D.; Muhuri, S.; Mimura, K.; Sugimoto, N., 2010: DNA junction structure stabilized by molecular crowding conditions. We examined the effects of molecular crowding conditions on the structures and thermodynamics of three-way junctions (TWJs) of DNA. To explore the crowding effects on the junction point, we further evaluated the number of water molecules associate...

Kim, S.Kee.; Hwang, K.; Huan, F.; Hwang, S.Ho., 2014: Particle size, temperature, and released amount of fat for safe periorbital fat grafts. The aim of this study was to determine the particle size, temperature, and amount of released fat for safe periorbital fat grafts. From 28 patients, fat was suctioned from the abdomen (large particles [LPs]) and from the inner thigh (small particl...

Kassanis, B.; Russell, G.E.; White, R.F., 1978: Seed and pollen transmission of beet cryptic virus in sugar beet plants. Cross pollination among infected and BCV-free plants showed that the virus was transmitted through both ovule and pollen. Mechanical inoculation of virus-free seedlings failed. BCV caused mild symptoms and appeared to reduce fresh wt. of roots.

Anonymous, 1978: Nitrite and the problem of nitrosamine formation. Fleischwirtschaft 58 (4) 602-603

Edwards, B.G., 1949: Ultraviolet spectra of some indole derivatives, including tryptophan and gramicidin. U.-v, absorption spectra of alcoholic solns. of gramicidin, methylol gramicidin, tryptophan, acetyltryptophan, and 2,3,4,5,-tetrahydro-4-carboline-5-carboxylic acid as well as of some indoles, methyl indoles and methylol indoles are presented. The...

Palazzouli V.; Mondillo S.; Fiorucci A.; Satta C., 1979: Consideration on jugular phlebogram in the complete heart block. In cases of complete heart block, simulating for long periods a block 2:1, the ventricular contractions and a double morphology of the jugular phlebogram with all its various components were recorded. The relation between the origin of wave C and...