+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Imatinib and docetaxel in combination can effectively inhibit glioma invasion in an in vitro 3D invasion assay

, : Imatinib and docetaxel in combination can effectively inhibit glioma invasion in an in vitro 3D invasion assay. Journal of Neuro-Oncology 101(2): 189-198

The main problem in the treatment of malignant astrocytomas is their invasive behaviour. Successful resection of the main tumour mass cannot prevent recurrence due to single cells invading the surrounding brain parenchyma at the time of diagnosis. The classical combination therapy, PCV (Procarbazine, CCNU and Vincristine) used for over 30 years; has shown its clinical effectiveness in the treatment of malignant astrocytomas and glioblastomas is still doubtful. Using an in vitro three dimensional invasion model, we tested the effect of the tyrosine kinase inhibitor imatinib and the microtubule inhibitor docetaxel on the invasion activity of a panel of astrocytic tumour cell lines, including two established glioma cell lines, IPSB-18 and SNB-19, and two primary cell lines, originating from glioblastomas, CLOM002 and UPHHJA, and in normal astrocytes. A dose response curve for each drug alone and in combination was determined. The half maximal inhibitory concentration (IC(50)) concentration of imatinib was between 15.7 and 18.7 μM, which did not affect invasion activity of the cell lines. The IC(50) concentration of docetaxel was between 0.7 and 19.8 nM, and at 14.9 nM docetaxel had a slight transient inhibitory effect on invasion activity of all tested cells. The combination of imatinib at 13.5 μM and docetaxel at 14.9 nM, however, synergistically inhibited cell growth and invasion activity and could not be reversed by drug removal. A combination treatment with tyrosine kinase inhibitors and cytotoxic drugs shows promise in tackling both glioma proliferation and invasion, and could present a new treatment regimen for malignant astrocytomas.


PMID: 20512610

DOI: 10.1007/s11060-010-0246-1

Other references

Babu, P.G.; Gnanamuthu, C.; Saraswathi, N.K.; Nerurkar, V.R.; Yanagihara, R.; John, T.J., 1993: HTLV-I-associated myelopathy in south India. Aids Research and Human Retroviruses 9(6): 499-500

Nascimento, A.A.; Araújo, F.G.; Gomes, I.D.; Mendes, R.M.M.; Sales, A., 2012: Fish gills alterations as potential biomarkers of environmental quality in a eutrophized tropical river in south-eastern Brazil. Gill anomalies in three common fish species of different taxonomic order, habitat dwelling and feeding habits (one Characiformes, Oligosarcus hepsetus; one Siluriformes, Hypostomus auroguttatus; and one Perciformes, Geophagus brasiliensis) from a...

Tworoger, S.S.; Hecht, J.L.; Giovannucci, E.; Hankinson, S.E., 2006: Intake of folate and related nutrients in relation to risk of epithelial ovarian cancer. Assessments of the relation between folate intake and ovarian cancer risk have been limited and inconsistent. Therefore, the authors prospectively examined the association of dietary and supplemental intakes of folate, methionine, and vitamin B-6...

Ebert, E.; Van Assche, C.J., 1970: Effects of triazine herbicides on the physiology of plants. 19: 823 and 20: 1791. The effect of triazines on auxin metabolism was studied in a series of in vivo and in vitro systems involving soyabean and tobacco tissues, leaf discs of Rumex, Sinapis, Taraxacum spp. and oats (senescence testing), wheat col...

Ringer, S., 1895: On a Preparation of Milk for Diabetic Patients. British Medical Journal 2(1823): 1412-1412

Lozeron, M.; Lin, C.C.; Mittendorf, R., 2000: Outcomes of small for gestational age and appropriate for gestational age infants less than 1500 grams. American Journal of Obstetrics & Gynecology 182(1 Part 2): S172, Jan

Bernasovska K.; Bernasovsky I.; Marcinekova A.; Vargova T.; Hanzelova V.; Balaz J., 1976: Sero anthropological study of gypsy children from podskalka in east slovakia czechoslovakia part 1 frequency of a 1 a 2 bo rh and mn blood group systems. Acta Facultatis Medicae Universitatis Brunensis (57): 283-292

Riske, F.J.; Smith, M.A.; Zhang, C.; White, K.H., 2010: Lysozyme retention on hydrophobic interaction chromatography predicts resin performance at large scale. It is critical that manufacturers understand the impact of resin variability on process performance and consistency. Choosing an appropriate resin lot often requires running manufacturing load material on a scale-down processing step and showing t...

Kwon, J-Hyeuk.; Lee, C-Jung., 2006: Rhizopus Soft Rot on Pear (Pyrus serotina) Caused by Rhizopus stolonifer in Korea. Rhizopus soft rot caused by Rhizopus stolonifer occurred on pears (Pyrus serotina) in the Jinju City Agricultural Products Wholesale Market in Korea from 2004 to 2005. The infection usually started from wounds due to cracking at harvest time. The...

Li, A.E.; Sneag, D.B.; Greditzer, H.G.; Johnson, C.C.; Miller, T.T.; Potter, H.G., 2016: Total Knee Arthroplasty: Diagnostic Accuracy of Patterns of Synovitis at MR Imaging. NlmCategory="UNASSIGNED">Purpose To determine the diagnostic accuracy of magnetic resonance (MR) imaging for differentiating synovial patterns in patients with total knee arthroplasty (TKA), whether diagnostic accuracy differs in inde...