+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Improvement of the method for methylmercury determination in aquatic products using liquid chromatography online coupled with atomic fluorescence spectrometry

, : Improvement of the method for methylmercury determination in aquatic products using liquid chromatography online coupled with atomic fluorescence spectrometry. Se Pu 29(7): 667-672

The improvement method was developed for methylmercury determination using liquid chromatography online coupled with cold vapor atomic fluorescence spectrometry (LC-CV-AFS). Cysteine was used as complexing agent in mobile phase instead of mercaptoethanol. Under the optimized conditions, baseline separation of mercury species could be achieved within 8 min on a C18 column with a mobile phase of 5% (v/v) acetonitrile-1 g/L L-cysteine-50 mmol/L ammonium acetate aqueous solution. The linear range of calibration curve of methylmercury was 1-50 microg/L and the limit of detection (S/N = 3) for methylmercury was 0.3 microg/L. Ultrasonication assisted hydrochloric acid extraction was used to extract methylmercury from seafood samples. The sample extract was cleaned up by a C18 solid phase extraction (SPE) cartridge. For validation of the method, certified reference materials and spiked seafood samples were analyzed. The determined methylmercury contents of certified reference materials NIST1566b, BCR464 and GBW10029 agreed well with the certified values. The determined methylmercury values for Food Analysis Performance Assessment Scheme (FAPAS) sample 07115 were satisfied. The recoveries of methylmercury in seafood samples at three spiked levels (10, 50 and 500 microg/kg) ranged from 89% to 112%, including cooked seafood food. The precision of the method based on relative standard deviation (RSD) was not more than 7%. The present method of LC-CV-AFS is accurate, sensitive, simple, and can meet the demand of methylmercury determination in seafood.


PMID: 22097794

Other references

Shaw, G.; Agathocleous, D.C., 1993: Some aromatic acyclonucleoside analogs. Antiviral Research 20(SUPPL 1): 103

Channing, A.; Moyer, D., C.; Howell, K., M., 2002: Description of a new torrent frog in the genus Arthroleptides from Tanzania (Amphibia, Anura, Ranidae). A new torrent frog in the genus Arthroleptides is described from the Udzungwa and Uluguru mountains in Tanzania. It is distinguished from the previously known species, A. dutoiti and A. martiensseni, by its large size, non-thickened supratympanic...

Herington A.C.; Smith A.I.; Wallace C.; Stevenson J.L., 1987: Partial purification from human serum of a specific binding protein for human growth hormone. Following the recent identification and characterization of a highly specific binding protein(s) for human growth hormone (hGH) in human sera, we now report our initial studies on its purification. Outdated blood blank serum was first fractionated...

Minkowitz, S.; Moskowitz, R.; Khafif, R.A.; Alderete, M.N., 1986: TRU-CUT needle biopsy of the breast. An analysis of its specificity and sensitivity. One hundred fifty needle core biopsies of the breast, performed with the use of the Travenol TRU-CUT disposable needle, were done from January 1980 to November 1984. There were 112 diagnoses of breast cancer and 33 diagnoses of normal breast tissu...

Littleboy, M.; Smith, D.M.; Bryant, M.J., 1996: Simulation modelling to determine suitability of agricultural land. Land suitability assesses the potential of land for a specific land use. Traditionally, interactions between land characteristics and productivity are determined using qualitative methods. In contrast, a quantitative land evaluation uses a biophys...

Peterson, Alvah., 1912: Anatomy of the tomato-worm larva, Protoparce Carolina. Annals of the Entomological Society of America, 5 246-269

Oota, Yukito, 1964: RNA in developing plant cells. Ann Rev Plant Physiol 15: 17-36

Friedrich, O.; Hund, E., 2006: Critical illness myopathy bei Intensivpatienten. Intensive care patients are at increased risk of developing sepsis with multi-organ failure during treatment (severe sepsis) possibly leading to complications of the central and peripheral nervous system. Among these, septic encephalopathy, criti...

Eysenck, H.J., 1943: Suggestibility And Hysteria. Journal of Neurology and Psychiatry 6(1-2): 22-31

Sharma, V.D.; Singh, S.P.; Taku, A., 1992: Purification of Salmonella stanley enterotoxin and its immunology and dermatotoxicity. Isolation of an enterotoxic factor from cell-free-culture-supernatant of S. stanley was achieved to homogeneity using salt precipitation, dialysis and molecular seive chromatography through Sephadex G-100 and G-200 columns. The purified enterotoxi...