+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Improving bgl1 gene expression in Saccharomyces cerevisiae through meiosis in an isogenic triploid

, : Improving bgl1 gene expression in Saccharomyces cerevisiae through meiosis in an isogenic triploid. Biotechnology Letters 36(6): 1279-1285

Introducing large numbers of target genes into the chromosome of Saccharomyces cerevisiae via δ-sequence-mediated integration is a good strategy for exploring the effects of gene dosage on expression and secretion of heterologous proteins. The expression of exogenous genes might be further improved through meiosis in an isogenic triploid. Here, a stable strain A-8 was screened from 35 sexual spore colonies obtained from an isogenic triploid integratively expressing bgl1 from Aspergillus aculeatus. The corresponding β-glucosidase activity in this strain was increased by ~120 % compared with the parent strain BGL-a. Measurement of doubling time, flow cytometry, and mating experiments further confirmed that A-8 was a spore-forming strain obtained from a triploid parent. Thus, combining δ-integration and meiosis in an isogenic triploid is a promising approach for improving the expression of exogenous proteins in S. cerevisiae.


PMID: 24563302

DOI: 10.1007/s10529-014-1471-z

Other references

Jaracz, S.; Chen, J.; Kuznetsova, L.V.; Ojima, I., 2005: Recent advances in tumor-targeting anticancer drug conjugates. Traditional cancer chemotherapy relies on the premise that rapidly proliferating cancer cells are more likely to be killed by a cytotoxic agent. In reality, however, cytotoxic agents have very little or no specificity, which leads to systemic toxi...

Casacchia, M.; Cappellini, R.; Meco, G., 1981: Recent acquisitions in therapy of Parkinson's disease. La Clinica Terapeutica 99(4): 401-411

Sharrock, J.T.R., 1969: Grey wagtail passage and population fluctuations in 1956-67. Bird Study, 16: 17-34

Kang S Y.; Kinsey R.A.; Rajan S.; Gutowsky H.S.; Gabridge M.G.; Oldfield E., 1981: Protein lipid interactions in biological and model membrane systems deuterium nmr of acholeplasma laidlawii b escherichia coli and cytochrome oxidase systems containing specifically deuterium labeled lipids. 2H-NMR spectra of A. laidlawii B (PG9) membranes and lipid extracts enriched biosynthetically in the presence of avidin with [14-2H3]tetradecan-1-oic acid, [16-2H3]hexadecan-1-oic acid, [4-2H2]-, [6-2H2]-, or [8-2H2]-tetradecan-1-oic acids, were r...

Zlotta, A.; Schulman, C., 1999: Cancer of the prostate: advances in the use of PSA and its derived indices--new tumor markers. This review illustrates the evolution in the clinical use of prostate specific antigen as a prostate cancer tumour marker. Because PSA lacks specificity, several new PSA parameters have been investigated such as the age-referenced PSA and the PSA...

Otahel, J.; Feranec, J.; Cebecauer, T., 2004: Landscape changes identified by application of the CORINE land cover databases: case study - part of the Zahorie lowland. Landscape changes are proper indicators of natural (climatic and hydrologic) processes, but above all of human influences. Changes of physical state of landscape, which is represented by land cover processed by the CORINE Land Cover (CLC) method,...

White, M.D.; Kuhn, N.J.; Ward, S., 1980: Permeability of lactating-rat mammary gland Golgi membranes to monosaccharides. The Golgi-membrane vesicles present in particulate preparations of lactating rat mammary gland were biosynthetically loaded with [14C]lactose. This lactose was effectively retained by particles sedimented after exposure to 0.25 M-disaccharide, but...

Sommerlade, L.; Henschel, K.; Wohlmuth, J.; Jachan, M.; Amtage, F.; Hellwig, B.; Lücking, C.Hermann.; Timmer, J.; Schelter, Börn., 2010: Time-variant estimation of directed influences during Parkinsonian tremor. The inference of interaction structures in multidimensional time series is a major challenge not only in neuroscience but in many fields of research. To gather information about the connectivity in a network from measured data, several parametric...

d Anglejan B.F., 1971: Submarine sand dunes in the estuary of the Saint Lawrence river. Abstracts with Programs - Geological Society of America 3(7): 536-537

Carbonin, P.U.; D.G.nnaro, M.; Pahor, M.; Bernabei, R.; Sgadari, A.; Gambassi, G., 1990: Cardiac aging, calcium overload, and arrhythmias. The effect of aging was tested on experimental ventricular arrhythmias in isolated heart preparations from normal Wistar rats (NWR). Wistar Kyoto rats (WKY), and spontaneously hypertensive rats (SHR). Delayed afterdepolarizations and triggered act...