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In ovo and in vitro susceptibility of American alligators (Alligator mississippiensis) to avian influenza virus infection


, : In ovo and in vitro susceptibility of American alligators (Alligator mississippiensis) to avian influenza virus infection. Journal of Wildlife Diseases 51(1): 187-198

Avian influenza has emerged as one of the most ubiquitous viruses within our biosphere. Wild aquatic birds are believed to be the primary reservoir of all influenza viruses; however, the spillover of H5N1 highly pathogenic avian influenza (HPAI) and the recent swine-origin pandemic H1N1 viruses have sparked increased interest in identifying and understanding which and how many species can be infected. Moreover, novel influenza virus sequences were recently isolated from New World bats. Crocodilians have a slow rate of molecular evolution and are the sister group to birds; thus they are a logical reptilian group to explore susceptibility to influenza virus infection and they provide a link between birds and mammals. A primary American alligator (Alligator mississippiensis) cell line, and embryos, were infected with four, low pathogenic avian influenza (LPAI) strains to assess susceptibility to infection. Embryonated alligator eggs supported virus replication, as evidenced by the influenza virus M gene and infectious virus detected in allantoic fluid and by virus antigen staining in embryo tissues. Primary alligator cells were also inoculated with the LPAI viruses and showed susceptibility based upon antigen staining; however, the requirement for trypsin to support replication in cell culture limited replication. To assess influenza virus replication in culture, primary alligator cells were inoculated with H1N1 human influenza or H5N1 HPAI viruses that replicate independent of trypsin. Both viruses replicated efficiently in culture, even at the 30 C temperature preferred by the alligator cells. This research demonstrates the ability of wild-type influenza viruses to infect and replicate within two crocodilian substrates and suggests the need for further research to assess crocodilians as a species potentially susceptible to influenza virus infection.

US$19.90

PMID: 25380354

DOI: 10.7589/2013-12-321


Other references

Wojdylo, L., 1964: Effect of water for the spring "Milo'S C" in Nal Ecz'Ow on the urinary excretion of electrolytes. Polski Tygodnik Lekarski 19: 1080-1083

Srivastava, R.P., 1997: Bark eating caterpillar a serious problem in fruit crops. This pest has a wide host range and has been observed to infect fruits trees, forest trees and even ornamental trees. The caterpillar feeds on bark, inside the plant vessels through which nutrition is transported and adversely effects the fruits b...

Toole, G.A.; Wilson, R.H.; Parker, M.L.; Wellner, N.K.; Wheeler, T.R.; Shewry, P.R.; Mills, E.N.C., 2006: The effect of environment on endosperm cell-wall development in Triticum aestivum during grain filling: an infrared spectroscopic imaging study. One of the major factors contributing to the failure of new wheat varieties is seasonal variability in end-use quality. Consequently, it is important to produce varieties which are robust and stable over a range of environmental conditions. Recen...

Bleidelis, E.; Germane, S.; Misane, I., 1992: IOS 1.1212 (Cerebrocrast)-induced normalizing effects of postnatal development of the offspring of alcoholized male rats: I. Offspring exposure to IOS 1.1212 treatment. IOS-1.1212 (cerebrocrast) intraperitoneal administration (0.005-5.0 mg/kg) to alcoholized paternal rat offspring (APO) during early postnatal period (from 20th to 30th day) significantly improved their learning and memory processes in conditional...

Kirzon, S.S.; Averkina, R.F.; Vyazor, O.E., 1970: Detection of lenticular antigens in the early stages of development of Rana temporaria. Byull. esksp. Biol. Med. 67: 1969: 506-508

Zhu, H.; Shi, J.P.; Zhao, C.J., 2005: Species composition, physiognomy and plant diversity of the tropical montane evergreen broad-leaved forest in southern Yunnan. Species composition, physiognomy and plant diversity of the less known tropical montane forests in southern Yunnan were studied based on the data from 15 sampling plots in three sites. These forests are mainly dominated by the families Theaceae, F...

Berman, S.A.; Salenitse, I.K., 1966: Parietal digestion in fishes. To elicit whether the mechanism of parietal (contact) digestion, as opposed to cavity digestion, is characteristic for the intestinal tract of fishes, experiments were set up on stomach-less carp. Perfusion of the intestine of the carp in vivo wit...

Becker, F.F., 1982: Morphological classification of mouse liver tumors based on biological characteristics. Examination of three strains of inbred mice suggested that specific morphological types of hepatic tumors are the result of genetic predisposition while other types of tumors are associated with exposure to chemical carcinogens. A simple classific...

Chan, D.C.; Pang, J.; Watts, G.F., 2015: Pathogenesis and management of the diabetogenic effect of statins: a role for adiponectin and coenzyme Q10?. There is growing evidence to suggest that statin therapy is associated with an increased risk of incident diabetes. The risk for statin-related diabetes depends upon many factors including age, pre-existing diabetic risk, type and potency of stati...

Kasarskis E.J.; Sparks D.L.; Slevin J.T., 1984: Neurochemical changes in hypothalamus during anorexia induced by zinc deficiency. Neurology 34(3 SUPPL 1): 198