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Induction of the Nrf2-driven antioxidant response by tert-butylhydroquinone prevents ethanol-induced apoptosis in cranial neural crest cells

, : Induction of the Nrf2-driven antioxidant response by tert-butylhydroquinone prevents ethanol-induced apoptosis in cranial neural crest cells. Biochemical Pharmacology 80(1): 144-149

Previous studies have shown that ethanol exposure causes apoptosis in cranial neural crest cells (NCCs), an ethanol-sensitive cell population implicated in Fetal Alcohol Spectrum Disorders (FASD). Additionally, induction of endogenous antioxidants through activation of nuclear factor-erythroid 2-related factor 2 (Nrf2) has been shown to prevent oxidative stress and apoptosis in ethanol-exposed mouse embryos. The objective of this study was to test whether tert-butylhydroquinone (tBHQ), an Nrf2 inducer, can protect NCCs against ethanol-induced apoptosis. Ethanol exposure was shown to cause a moderate increase in the protein expression of Nrf2 and its downstream antioxidants in the NCCs. Treatment of NCCs with tBHQ alone significantly increased the protein expression of Nrf2 and its downstream antioxidants and also significantly increased the activities of the antioxidant enzymes. In NCCs exposed to ethanol, the tBHQ-mediated antioxidant response prevented oxidative stress and apoptosis. These results clearly demonstrate that the activation of Nrf2 signaling confers protection against ethanol-induced apoptosis in NCCs.


PMID: 20223225

DOI: 10.1016/j.bcp.2010.03.004

Other references

Alawaji H.A., 1999: Swell and compressibility characteristics of sand-bentonite mixtures inundated with liquids. The swell behavior of expansive soils may be desirable as in many hydraulic containment applications or unfavourable and may cause major deterioration and distresses in many geotechnical applications such as highways and shallow foundations on exp...

Sidak, V.; Segui, E., 1978: Variation in Panicum maximum Jacq and some results of selection. See previous abstract.

Jacob, F., 1951: Experimental inhibition of growth of bacteria during the development of bacteriophage in Pseudomonas pyocyanea. Comptes Rendus Hebdomadaires des Seances de L'academie des Sciences 232(17): 1605-1607

Murayama, S.; Saito, Y.; Kanazawa, I.; Ihara, Y., 1998: Immunocytochemical application of anti-alpha-synuclein-antibody to human neurodegenerative disorders causing dementia. Society for Neuroscience Abstracts 24(1-2): 965

Collins, K., 1984: Dressed to compete: angora goats fight for a share of fiber markets. Farmline United States Dept of Agriculture Economic Research Service 5(9): 16-17

Tsuno, K., 1961: Effects of various amino acids on calcium absorption from isolated surviving small intestine of rats. The effect of various amino acids added to calcium solution on the absorption of Ca from the isolated surviving small intestine was studied. The addition of valine showed a marked increase of Ca absorption. Ca absorption in isolated small intestin...

Okuma, Y.; Yokotani, K.; Murakami, Y.; Osumi, Y., 1998: Properties of calcium channels coupled to endogenous glutamate release from the vascularly perfused rat stomach in vitro. We have demonstrated that both high-K+ and electrical stimulation of the vagus nerves release endogenous glutamate from the vascularly-perfused rat stomach in a calcium-dependent manner. In the present study, we examined properties of calcium chan...

Oehlenschlager, J., 1986: A universal method of estimating fat content in fishes and other marine animals. A version of the 3-phase system based on water:chloroform:methanol for estimating fat content of fishes and fish products over the entire range from less than 0.5 to 35% is described. Modifications of the original method involve suspension of the...

Anonymous, 1979: Economic use of oil-fired boiler plant. Losses arising from the inefficient operation of oil fired boiler plant are discussed, from oil delivery to the final heat output, taking a medium capacity installation having an oil bill of pounds-sterling 200 000/yr as an example.

Ryhanen, L.; Graves, P.N.; Bressan, G.M.; Prockop, D.J., 1978: Synthesis of an elastin component of molecular weight about 70000 by polysomes from chick embryo aortas. Polysomes were isolated from aortas of 17 day old chick embryos, and synthesis of nascent polypeptide chains was completed in vitro. When a mixture of labeled amino acids found in elastin was used, the major radioactive product obtained was of MW...