geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Influence of mesenchymal stem cells on the response of endothelial cells to laminar flow and shear stress


, : Influence of mesenchymal stem cells on the response of endothelial cells to laminar flow and shear stress. Cells, Tissues, Organs 198(4): 289-299

The interactions between endothelial cells (ECs) and smooth muscle cells (SMCs) in a complex hemodynamic environment play an important role in the control of blood vessel function. Since autologous SMCs are not readily available for the tissue engineering of a blood vessel substitute, a substitute for SMCs, such as human adult bone marrow-derived mesenchymal stem cells (MSCs), is needed. The objective of this study was to use a three-dimensional coculture model of the blood vessel wall, comprised of ECs and MSCs, to determine how the presence of MSCs affects EC function. Two vascular coculture models with an EC monolayer were created using type I collagen. All models were exposed to steady laminar flow with a shear stress of 15 dyn/cm(2) for up to 48 h. ECs in both the MSC and SMC coculture models expressed up-regulated EC-specific markers compared to the EC-only control model. The most dramatic difference observed between the two coculture models was in the experiments assessing monocyte adhesion. Here, fewer monocytes bound after laminar shear compared to static conditions; however, the number of bound monocytes was much lower for the EC-MSC coculture model than the EC-SMC coculture model for both static and shear conditions. These results suggest the feasibility of developing a tissue-engineered blood vessel substitute using MSCs as a substitute for SMCs.

US$19.90

PMID: 24335258

DOI: 10.1159/000356319


Other references

Fallah, R.; Karbasi, S.Akhavan., 2011: Recurrence of febrile seizure in Yazd, Iran. Febrile seizure (FS) is the most common problem in pediatric neurology. The purpose of this study was to determine FS recurrence frequency and to evaluate its risk factors. In a descriptive retrospective study, 139 children with first FS, admitted...

Egekeze J.O.; Oehme F.W., 1980: Thio cyanate to cyanide revisited. Clinical Toxicology 16(1): 127-128

Ferreira Montero, V.; Eiras Ajuria, J.; Gómez Pereda, R.; Montori Lasilla, M.; Valero Palomero, M.I., 1983: Cerebral metastasis, idiopathic pneumoperitoneum and obstructive jaundice as clinical manifestations of carcinoma of the pancreas. A case of undifferentiated pancreatic carcinoma is described. The first symptoms were due to brain metastases and was then diagnosed as malignant glioma. The appearance of a sudden obstructive jaundice and pneumoperitoneum without any apparent cau...

Mankinen Edward A.; Irwin William P.; Gromme C.S.erman, 1984: Implications of paleomagnetism for the tectonic history of the eastern Klamath and related terranes in California and Oregon. Field Trip Guidebook - Pacific Section, Society of Economic Paleontologists and Mineralogists 42: 221-229

Shibasaki-Kitakawa, N.; Honda, H.; Kuribayashi, H.; Toda, T.; Fukumura, T.; Yonemoto, T., 2006: Biodiesel production using anionic ion-exchange resin as heterogeneous catalyst. The transesterification reactions of triolein with ethanol using various ion-exchange resin catalysts were conducted to produce ethyl oleate as a biodiesel. The anion-exchange resins exhibited much higher catalytic activities than the cation-excha...

Sengers, R.C.A., 1967: Influence of erythrocyte concentration and cation levels on the hemolytic action of staphylococcal -hemolysin. J Bacteriol 94(5): 1791-1792

Maxell, B.; Hokit, G., 1999: Amphibians reptiles. Through their interactions with other organisms, amphibians and reptiles are key components of many ecological systems. Consistent with the overall decline in biodiversity, amphibian and reptile populations have recently, or are currently, experie...

Narasimhulu, S., 1979: Constraint on the substrate cytochrome P-450 binding reaction in bovine adrenocortical microsomes at physiological temperature. The addition of cholate to the microsomes at 37.5 degrees C resulted in a striking decrease in the apparent substrate dissociation constant (K's) and its temperature dependency. The microsomal membranes depleted of 80% of the lipids preserved...

Servadei A., 1973: The rhynchota of malenco valley italy heteroptera and hemiptera auchenorrhyncha. Thirty-four species from the Rhynchota Valley in Italy were collected. Species presently known number 215; 139 are Heteroptera and 76 are Homoptera. Of the taxa listed, 109 are new for Lombardia and 8 are new for Italy [Psallus betuleti f. nigrico...

Kane, D.W.; Hohman, M.M.; Cerami, E.G.; McCormick, M.W.; Kuhlmman, K.F.; Byrd, J.A., 2006: Agile methods in biomedical software development: a multi-site experience report. Background: Agile is an iterative approach to software development that relies on strong collaboration and automation to keep pace with dynamic environments. We have successfully used agile development approaches to create and maintain biomedical...