geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Inhibitory effect of intracellular lipid load on macroautophagy


, : Inhibitory effect of intracellular lipid load on macroautophagy. Autophagy 6(6): 825-827

Degradation of intracellular components via macroautophagy is a complex multistep process that starts with the sequestration of cytosolic cargo in a de novo formed double-membrane vesicle or autophagosome. This compartment acquires the hydrolases required for cargo digestion by fusion with lysosomes. In contrast to the detailed molecular dissection of the components that participate in the induction, regulation and execution of the early steps in macroautophagy through the engulfment of cargo in autophagosomes, the mechanisms involved in the lysosomal clearance of autophagosomes have been poorly characterized in mammals. One of the major limitations in this respect has been the fact that autophagosome-lysosome fusion in intact cells involves several independent steps, namely binding of the molecular motors associated with the surface of the vesicles with the cytoskeletal network, directional vesicular trafficking and fusion between the two vesicular compartments. Furthermore, both lysosomes and autophagosomes are very dynamic organelles that can fuse with different vesicular structures involved in macroautophagy, but also along the endocytic and phagocytic pathways. To resolve these limitations and directly analyze the fusion step between autophagosomes and different compartments of the endocytic-lysosomal pathway, we have recently developed an in vitro fusion assay with autophagosomes, lysosomes and endosomes isolated from cells or tissues. Fluorescent labeling of these compartments allows for the tracking of fusion events by fluorescence microscopy or by fluorescence-activated cell sorting (FACS). Labeling of either membrane proteins on the surface of the organelles or dye-loading of the vesicles permits the monitoring of hemi-membrane fusion and complete vesicular fusion (cargo mixing).

US$19.90

PMID: 20647740

DOI: 10.1096/fj.09-144519


Other references

Sugihara H.; Nikai T.; Nakamura T.; Daido H.; Tanaka T., 1980: Enzymochemical studies on snake venoms purification and properties of anti coagulant factors of anti coagulant 1 and 2 in the venom of agkistrodon acutus. Two anticoagulant factors, anticoagulant 1 (Al) and anticoagulant 2 (A2), were isolated from the venom of A. acutus by a combination of gel filtration on Sephadex G-75 and chromatography on CM-Sephadex C-50 and DEAE-Sephacel. By these procedures,...

Trapeznikova, M.F.; Bazaev, V.V.; Urenkov, S.B., 2004: A comparative analysis of the results of open endoscopic operations in posterior urethra obliterations in men. Endoscopic recanalization of posterior urethra (PU) obliteration (a novel method) was compared to open reconstructive-plastic operations. 242 patients with PU and vesicular cervix obliterations entered the study. 93 patients of group 1 have underg...

Xu, C.H., 1986: Effect of shengmai san on CH3-3H-thymidine, 5-3H-uracil incorporation into cultured liver cells in vitro. Zhong Yao Tong Bao 11(3): 47-48

Czaika, F., 1961: Complications in follow-up treatment of infants with surgically treated esophageal atresia. Langenbecks Archiv für Klinische Chirurgie ... Vereinigt Mit Deutsche Zeitschrift für Chirurgie 298: 578-582

Seidel, C.; Herrmann, R.; Hoess, E.; Batz, H.G.org, 2001: Specific binding substances for antibodies and their use for immunoassays or vaccines. The invention relates to derivatives of hepatitis C virus amino acid sequences. These derivatives can be used to screen samples, such as blood, to determine if antibodies to hepatitis C virus are present.

Petersen, N.; Davies, D.S., 1953: The square flap in repair of unilateral hare-lip. South African Medical Journal 27(49): 1114-1115

op't Hof, J., 1977: Genetic disease--risks of occurrence and recurrence. Sa Nursing Journal. Sa Verplegingstydskrif 44(11): 15-16

McKeown, C.; Thompson, J., 2001: Clinical governance implementing clinical supervision. IN 1993 THE Department of Health published A Vision for the Future which included a tar- get related specifically to clinical supervision. Subsequently, the United Kingdom Central Council for Nursing, Midwifery and Health Visiting approved its ini...

Rego, J.L.; Meselhe, E.; Stronach, J.; Habib, E., 2010: Numerical modeling of the Mississippi-Atchafalaya Rivers sediment transport and fate; considerations for diversion scenarios. Journal of Coastal Research 26.2

Buytendijk, F.J.J., 1909: On the consumption of oxygen by cold-blooded animals in connection with their size. Proceedings of the Section of Sciences K Akademie van Wetenschappen te Amsterdam, 12 48-54