+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Interactions of AsCy3 with cysteine-rich peptides

, : Interactions of AsCy3 with cysteine-rich peptides. Organic Letters 16(14): 3824-3827

There is great interest in fluorogenic compounds that tag biomolecules within cells. Biarsenicals are fluorogenic compounds that become fluorescent upon binding four proximal Cys thiols, a tetracysteine (Cys(4)) motif. This work details interactions between the biarsenical AsCy3 and Cys(4) peptides. Maximal affinity was observed when two Cys-Cys pairs were separated by at least 8 amino acids; the highest affinity ligand bound in the nanomolar concentration range (K(app) = 43 nM) and with a significant (3.2-fold) fluorescence enhancement.


PMID: 24999741

DOI: 10.1021/ol501721j

Other references

Perez-Higareda, G., 1981: Additions to the knowledge of the amphibians of the Estacion de Biologia Tropical Los Tuxtlas UNAM, Veracruz, Mexico. Bulletin of the Maryland Herpetological Society, 172: 61-63

Janssens, L.A., 1992: Acupuncture for the treatment of thoracolumbar and cervical disc disease in the dog. Thoracolumbar disc disease (TLDD) and cervical disc disease (CDD) occur quite regularly in veterinary small animal practice, occurring predominantly in chondrodystrophic breeds and mostly around the age of 5-6 years. CDD is responsible for +/- 15%...

Wendt, C.; Floss, H.; Krause, C.; Rueden, H., 1997: Screening and susceptibility testing of vancomycin-resistant enterococci in a small laboratory. Abstracts of the General Meeting of the American Society for Microbiology 97(0): 122

Takai, K., 2006: Possible conformations of 5-aminomethyluridine derivatives recognizing a G at the third position of the codon. Specificity of the codon-anticodon interaction is often modulated by post-transcriptional modification at the first position of the anticodon (position 34) of the tRNA molecules. The modification of U(34) into 5-aminomethyluridine derivatives faci...

Kate A.R.tliff; Bregje A.P.S.inkels; Kimberly Klerx; Brian A.N.sek, 2012: Does One Bad AppleJuice Spoil the Bunch? Implicit Attitudes Toward One Product Transfer to Other Products by the Same Brand. If people like a product, they will automatically like another product from the same brand even if they do not know anything about it (demonstrated in Study 1). In one sense, this may be a reasonable inference brands that have one good product may...

Wallick, E.T.; Allen, J.C.; Schwartz, A., 1973: Differential effects of metal chelators on Na plus, K plus-ATPase activity. Archives of Biochemistry and Biophysics 158(1): 149-153

Igamberdyeva G.O., 1988: The adequacy of the diet in preschool institutions in the city of tashkent uzbek ssr ussr. Meditsinskii Zhurnal Uzbekistana (7): 16-17

Oguzturk, H.; Ozgur, D.; Turtay, M.G.; Kayaalp, C.; Yilmaz, S.; Dogan, M.; Pamukcu, E., 2013: Tramadol or paracetamol do not effect the diagnostic accuracy of acute abdominal pain with significant pain relief - a prospective, randomized, placebo controlled double blind study. To examine the effects of early administration of analgesics in patients with acute abdominal pain on pain severity, abdominal findings and diagnostic accuracy. 210 patients with non-traumatic acute abdominal pain lasting less than 72 hours were e...

Brown, S.C.S., 1938: Nomenclature of the Coniferae. The confusion in regard to the nomenclature of the Coniferae is due to Linnaeus' reversing the silver firs (Abies) and spruce firs (Picea). Meyrick gives P. excelsa as the common food for Dioryctria splendidella H. S. (Lep.), while in another...

Rattner, H., 1953: The Department of Dermatology; Northwestern University Medical School. Quarterly Bulletin. Northwestern University . Medical School 27(2): 145-148