geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Interleukin-12-anchored exosomes increase cytotoxicity of T lymphocytes by reversing the JAK/STAT pathway impaired by tumor-derived exosomes


, : Interleukin-12-anchored exosomes increase cytotoxicity of T lymphocytes by reversing the JAK/STAT pathway impaired by tumor-derived exosomes. International Journal of Molecular Medicine 25(5): 695-700

Tumor-derived exosomes express tumor antigens, leading to their promising utility as tumor vaccines, but they also can suppress T-cell signaling molecules and reduce cytotoxic effects. We investigated whether interleukin-12 (IL-12)-anchored exosomes (EXO/IL-12) reverse tumor exosome-mediated inhibition of T-cell activation and cytotoxicity was associated with inhibition of JAK3 and p-STAT5. A co-expression plasmid of pBudCE4.1/IL-12A/ IL-12B-GPI was constructed. EXO/IL-12 was identified by transmission electron microscopy and Western blotting, which induced proliferation and cytotoxicity of T-cells and were analyzed by CFSE-based flow cytometry. Expression of JAK2, JAK3 and p-STAT5 was detected by Western blotting. Our results showed that EXO/IL-12 was much more efficient in induction of the proliferation, release of IFN-gamma and cytotoxic effect of T lymphocytes than conventional exosomes in vitro. Exosomes inhibited the expression of JAK3 and phosphorylation of STAT5 in high doses in T-cells, but not JAK2, while EXO/IL-12 had much less attenuated reduction of the expression of p-STAT5. The enhanced cytotoxic effects of T lymphocytes might partly depend on EXO/IL-12 reversing the suppressed expression of p-Stat5 by Jak2/Stat5 pathway. These findings might provide an alternative approach for developing exosomes into tumor vaccines.

US$29.90

PMID: 20372811


Other references

Hilbink F.W.; Van Roozelaar D.J., 1988: A micro assay and neutralization test for the virus of infectious laryngotracheitis their reproducibility and the effect of various testing conditions. The reproducibility of micro-assays for ILTV was assessed. The standard deviation of the virus assay in three successive determinations was 0.4, 0.4 and 0.3 log TCID50. The micro-neutralization test produced standard deviations of 0.6, 0.8, 0.5, 1...

Bsteh, G.; Ehling, R.; Walchhofer, L-Maria.; Hegen, H.; Auer, M.; Wurth, S.; Di Pauli, F.; Wagner, M.; Reindl, M.; Deisenhammer, F.; Berger, T., 2017: Paroxysmal and unusual symptoms as first clinical manifestation of multiple sclerosis do not indicate benign prognosis-The PaSiMS II study. Paroxysmal (PS) and unusual symptoms (US) account for approximately 1.6% of initial manifestations of multiple sclerosis (MS) and have comparable conversion rates to clinically definite MS (CDMS) as classical bout onset symptoms (CS). However, lon...

Arnold, GW.; Steven, D.; Weeldenburg, J.; Brown, OE., 1986: The use of alpha-chloralose for the repeated capture of western grey kangaroos, Macropus fuliginosus. Alpha-chloralose was used for 9 years in a study of growth, development and population size of western grey kangaroos, Macropus fuliginosus. Grain and water at accustomed feeding and watering points were drugged at intervals, for one night at a ti...

Folz, B.J., 2016: Comment on the publication of Hansson, Halling and Fangerau with the title 'The Nobel Prize and Otolaryngology: "Papa Gunnar's" promotion of his peers Gustav Killian and Themistocles Gluck'. Acta Oto-Laryngologica 136(11): 1196-1196

Maus, U.; Henning, S.; Wenschuh, H.; Mayer, K.; Seeger, W.; Lohmeyer, J., 2002: Role of endothelial MCP-1 in monocyte adhesion to inflamed human endothelium under physiological flow. Monocyte chemoattractant protein-1 (MCP-1) is an essential chemokine involved in monocyte traffic across endo- and epithelial barriers both in vitro and in vivo. However, the contribution of endothelial MCP-1 signaling via its CCR2 receptor in mon...

Fall, T.; Hägg, S.; Ploner, A.; Mägi, R.; Fischer, K.; Draisma, H.H.M.; Sarin, A-Pekka.; Benyamin, B.; Ladenvall, C.; Åkerlund, M.; Kals, M.; Esko, Tõnu.; Nelson, C.P.; Kaakinen, M.; Huikari, V.; Mangino, M.; Meirhaeghe, A.; Kristiansson, K.; Nuotio, M-Liisa.; Kobl, M.; Grallert, H.; Dehghan, A.; Kuningas, M.; de Vries, P.S.; de Bruijn, Rée.F.A.G.; Willems, S.M.; Heikkilä, K.; Silventoinen, K.; Pietiläinen, K.H.; Legry, V.; Giedraitis, V.; Goumidi, L.; Syvänen, A-Christine.; Strauch, K.;, 2015: Age- and sex-specific causal effects of adiposity on cardiovascular risk factors. Observational studies have reported different effects of adiposity on cardiovascular risk factors across age and sex. Since cardiovascular risk factors are enriched in obese individuals, it has not been easy to dissect the effects of adiposity fro...

Hegdé, J.; Stephenson, E.C., 1993: Distribution of swallow protein in egg chambers and embryos of Drosophila melanogaster. The Drosophila maternal effect gene swallow has a role in localizing bicoid mRNA at the anterior margin of the oocyte during oogenesis, and a poorly characterized role in nuclear divisions in early embryogenesis. We have examined the distribution...

Borgeat, A.; Pizzolato, G.; Cox, J.N.; Suter, P.M., 1992: Latent dystrophic myopathy revealed by unsuccessful weaning from mechanical ventilation. We report a patient who had difficulty in weaning from mechanical ventilation which was due to an unsuspected latent dystrophic myopathy. The association of latent dystrophic myopathy and unsuccessful weaning has not been previously reported.

Durham, H.W., 1944: New Volcanoes And A New Mountain Range. Science 100(2586): 49-50

Johnson A.L.; Gregory G.B.; Ripka W.C., 1986: Synthesis of imidazoles pendant to a quaternary carbon center. Abstracts of Papers American Chemical Society 192