geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Interleukin-6 attenuates insulin-mediated increases in endothelial cell signaling but augments skeletal muscle insulin action via differential effects on tumor necrosis factor-alpha expression


, : Interleukin-6 attenuates insulin-mediated increases in endothelial cell signaling but augments skeletal muscle insulin action via differential effects on tumor necrosis factor-alpha expression. Diabetes 58(5): 1086-1095

The cytokine interleukin-6 (IL-6) stimulates AMP-activated protein kinase (AMPK) and insulin signaling in skeletal muscle, both of which result in the activation of endothelial nitric oxide synthase (eNOS). We hypothesized that IL-6 promotes endothelial cell signaling and capillary recruitment in vivo, contributing to increased glucose uptake. The effect of IL-6 with and without insulin on AMPK, insulin, and eNOS signaling in and nitric oxide (NO) release from human aortic endothelial cells (HAECs) was examined. The physiological significance of these in vitro signaling events was assessed by measuring capillary recruitment in rats during control and euglycemic-hyperinsulinemic clamps with or without IL-6 infusion. IL-6 blunted increases in insulin signaling, eNOS phosphorylation (Ser1177), and NO production and reduced phosphorylation of AMPK in HAEC in vitro and capillary recruitment in vivo. In contrast, IL-6 increased Akt phosphorylation (Ser473) in hindlimb skeletal muscle and enhanced whole-body glucose disappearance and glucose uptake during the clamp. The differences in endothelial cell and skeletal muscle signaling were mediated by the cell-specific, additive effects of IL-6 and insulin because this treatment markedly increased tumor necrosis factor (TNF)-alpha protein expression in HAECs without any effect on TNF-alpha in skeletal muscle. When HAECs were incubated with a TNF-alpha-neutralizing antibody, the negative effects of IL-6 on eNOS signaling were abolished. In the presence of insulin, IL-6 contributes to aberrant endothelial cell signaling because of increased TNF-alpha expression.

US$19.90

PMID: 19188427

DOI: 10.2337/db08-0775


Other references

Ukkonen, P.; Pettersson, R.; Halonen, P., 1979: Hepatitis A antibodies in Finland. Serum specimens from 191 Finnish adults were tested for antibodies to hepatitis A virus (anti-HAV). The frequency of anti-HAV increased from 8% in the age group of 20-29 years to 92% in those older than 60 years. The level of 50% was reached betwe...

Gillet, J.Y.; Skoulios, G.; Wolff, F.; Vors, J.; Schumacher, J.C., 1969: Use of a technic for the rapid determination of urinary estriol in the supervision of pathological pregnancies. Bulletin de la Federation des Societes de Gynecologie et Dobstetrique de Langue Francaise 21(5): 585-586

Li, W-Fang.; Yu, J.; Ma, X-Xiao.; Teng, Y-Bin.; Luo, M.; Tang, Y-Jun.; Zhou, C-Zhao., 2010: Structural basis for the different activities of yeast Grx1 and Grx2. Yeast glutaredoxins Grx1 and Grx2 catalyze the reduction of both inter- and intra-molecular disulfide bonds using glutathione (GSH) as the electron donor. Although sharing the same dithiolic CPYC active site and a sequence identity of 64%, they ha...

Lin Nian feng, 1980: On medico-hydrogeochemistry. Shuiwendizhi Gongchengdizhi = Hydrogeology and Engineering Geology 1980(6): 61-62

Mauss, E., 1974: The responsibility is lasting. Winzer 30 (1) 1-2

Liang, Y.; Jiang, X-Cheng.; Liu, R.; Liang, G.; Beyer, T.P.; Gao, H.; Ryan, T.P.; Dan Li, S.; Eacho, P.I.; Cao, G., 2004: Liver X receptors (LXRs) regulate apolipoprotein AIV-implications of the antiatherosclerotic effect of LXR agonists. Liver X receptors (LXRs) regulate target genes that are critical in lipoprotein metabolism and atherosclerosis. Apolipoprotein AIV (ApoAIV) is an apolipoprotein that is associated with chylomicrons and high-density lipoproteins. Plasma ApoAIV leve...

Anonymous, 1941: Charles Value Chapin, 1856-1941. American Journal of Public Health and the Nation's Health 31(3): 264-265

Yanson, L.K.; P.P., 1956: An apparatus for quantitative determination of thermic stimulation. To effect thermic stimulation, the author proposes a circle of pure Zn, of a specific thermal heat capacity of 0.0925 cal/g to 1[degree], as a thermode. The instrument is connected with a thermoelement and a galvanometer. [long dash]Transl. from R...

Gevers T.W., 1969: The tin-bearing pegmatites of the Erongo area, South West Africa. Pages 138-140 1969

Middelburg, J.J.; Nieuwenhuize, J., 2001: Nitrogen isotope tracing of dissolved inorganic nitrogen behaviour in tidal estuaries. Concentration versus salinity diagrams are the most widely used method to derive estuarine sources and sinks of nitrogen. This method can not distinguish conservative mixing due to a lack of activity, from that due to an approximate balance of sou...