+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Islet amyloid polypeptide in pancreatic islets from type 1 diabetic subjects

, : Islet amyloid polypeptide in pancreatic islets from type 1 diabetic subjects. Islets 3(4): 166-174

Islet amyloid polypeptide is originally isolated as the chief constituent of amyloid deposits in type 2 diabetic islets. Islet amyloid polypeptide hyposecretion was known in type 1 diabetics and this study aimed to detect possibly reduced islet amyloid polypeptide-positive cells in type 1 diabetic islets. Non-diabetic control islets showed about 60% of islet cells were insulin cells, and 60% of insulin cells were positive for IAPP. In type 1 diabetic islets, islets were generally smaller than control islets, consisting of weaker positive cells for insulin and islet amyloid polypeptide. Medium-sized islets still retained some insulin positive cells, whereas islet amyloid polypeptide positive cells were much less or even absent, but some insulin-negative cells were weakly islet amyloid polypeptide positive. An occasional extra-large islet, representing regenerating islets, consisting of more than 100 islet cells revealed less than 35% insulin and 20% islet amyloid polypeptide positive cells with relatively increased glucagon and somatostatin cells. Both normal and type 1 diabetic islets revealed scattered, densely insulin and islet amyloid polypeptide positive sickle-shaped cytoplasm without granular appearance, consistent with degenerating insulin cells. Using commercially available rabbit anti-islet amyloid polypeptide antibody, immunostaning was performed on ten cases of type 1 diabetic pancreata and eight non-diabetic controls. Both control and type 1 diabetic pancreata were systematically immunostained for insulin, glucagon, somatostatin and islet amyloid polypeptide. Control islets consisted of about 60% insulin cells, and about 34% of islet cells were amyloid polypeptide positive with scattered and densely positive for insulin and islet amyloid polypeptide without granular appearance, consistent with degenerating β cells. All islets, including occasional extra-large islets from type 1 diabetics, showed less insulin cells and less islet amyloid polypeptide positive cells with twice increased glucagon and somatostatin cells of the control islets, but some insulin-negative cells were positive for islet amyloid polypeptide, suggesting the presence of islet amyloid polypeptide in degenerating and extra-large regenerating islets. Thus, this immunocytochemical staining revealed generally less islet amyloid positive cells in type 1 diabetic islets, corresponding to severe hyposecretion of islet amyloid polypeptide in type 1 diabetics.


PMID: 21633185

Other references

Phizackerley P.J.R., 1972: Inhibition by ascorbic acid of the sodium plus potassium ion stimulated atpase in rat brain. Biochemical Journal: 139p

Gruber, G.B.; Vo.R.Kitansky, K., 1960: Karl von ROKITANSKY: in memorian (1804-1878). Zentralblatt für Allgemeine Pathologie U. Pathologische Anatomie 101: 513-516

Wolfart, W.; Eckelmann, I., 1964: Repeated Resections In Pulmonary Tuberculosis. Praxis der Pneumologie 18: 346-362

Yoo, P.D.; Shwen Ho, Y.; Ng, J.; Charleston, M.; Saksena, N.K.; Yang, P.; Zomaya, A.Y., 2011: Hierarchical kernel mixture models for the prediction of AIDS disease progression using HIV structural gp120 profiles. Changes to the glycosylation profile on HIV gp120 can influence viral pathogenesis and alter AIDS disease progression. The characterization of glycosylation differences at the sequence level is inadequate as the placement of carbohydrates is struc...

Wright, A.F., 2001: How evidence gets published. Occasional Paper: 7-9

Zepezauer, V.; Schacht, K.H.; Bocklisch, H., 1985: Pathomorphology of mercury poisoning in swine. Pigs submitted with suspected mercury poisoning had mercury concentrations of 14.3-45.2 mg/kg in liver and 30.5-51.9 in kidney. The pathological picture was variable, only moderately specific, and similar to that of swine fever. The clinical and e...

Linke, T.; Ross, A.Catharine.; Harrison, E.H., 2006: Proteomic analysis of rat plasma by two-dimensional liquid chromatography and matrix-assisted laser desorption ionization time-of-flight mass spectrometry. The proteomic analysis of plasma and serum samples represents a formidable challenge due to the presence of a few highly abundant proteins such as albumin and immunoglobulins. Detection of low abundance protein biomarkers therefore requires either...

Peeraphatdit, T.; Smyrk, T.C.; Alexander, G.L., 2017: Recurrent Jejunal Intussusception Caused by Heterotopic Pancreas Mass. Clinical Gastroenterology and Hepatology 15(12): E175-E176

Baldasso, N.A., 2004: Sustainable local development. This document summarizes 5 years of experiences and observations pertaining to planning and local development in the administrative region of Porto Alegre, Brazil. It describes practical aspects related to the various stages of planning, from moti...

Mahnken, A.H.; Spuentrup, E.; Niethammer, M.; Buecker, A.; Boese, J.; Wildberger, J.E.; Flohr, T.; Sinha, A.M.; Krombach, G.A.; Günther, R.W., 2003: Quantitative and qualitative assessment of left ventricular volume with ECG-gated multislice spiral CT: value of different image reconstruction algorithms in comparison to MRI. To assess the value of different image reconstruction algorithms for assessment of the left ventricular function using retrospectively ECG-gated multislice spiral computed tomography (MSCT) of the heart. MSCT and cine magnetic resonance (MR) imagi...