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Islet amyloid polypeptide is not a target antigen for CD8+ T-cells in type 2 diabetes


, : Islet amyloid polypeptide is not a target antigen for CD8+ T-cells in type 2 diabetes. Iranian Journal of Immunology 11(1): 1-12

Type 2 diabetes (T2D) is a chronic metabolic disorder in which beta-cells are destroyed. The islet amyloid polypeptide (IAPP) produced by beta-cells has been reported to influence beta-cell destruction. To evaluate if IAPP can act as an autoantigen and therefore, to see if CD8+ T-cells specific for this protein might be present in T2D patients. Peripheral blood mononuclear cells (PBMC) were obtained from human leukocyte antigen (HLA)-A2+ T2D patients and non-diabetic healthy subjects. Cells were then screened for peptide recognition using ELISPOT assay for the presence of IFN-γ producing CD8+ T-cells against two HLA Class I-restricted epitopes derived from IAPP (IAPP5-13 and IAPP9-17) and common viral antigenic minimal epitopes Flu MP 58-66, CMV495-503, EBV280-288 and HIV77-85 as controls. A total of 36.4% of patients and 56.2% of healthy subjects showed a response against IAPP5-13 peptide. No significant difference in response against this peptide was noted between the patients and the healthy donors. With respect to peptide IAPP9-17, although healthy subjects showed a higher mean number of spot forming cells than the patients, the difference was not significant; 36.4% of patients and 37.5% of controls responded to this peptide. The response of healthy subjects to the common viral peptides was stronger than that of the patients, though the result was not significant. It is unlikely that IAPP would be a target for CD8+ T-cells in diabetic patients; however, the trend observed toward a lower response of T2D patients against IAPP and common viral peptides may imply a decreased immune response in these patients.

US$19.90

PMID: 24632583

DOI: IJIv11i1A1


Other references

Zheng, X.; Li, A.; Zhao, L.; Zhou, T.; Shen, Q.; Cui, Q.; Qin, X., 2013: Key role of microRNA-15a in the KLF4 suppressions of proliferation and angiogenesis in endothelial and vascular smooth muscle cells. While recent insights indicate that the transcription factor Krüppel-like factor 4 (KLF4) is indispensable for vascular homeostasis, its exact role in proliferation and angiogenesis and how it functions remain unresolved. Thus, the aim of the pre...

Asad, M.Azeem.; Xia, X.; Wang, C.; He, Z., 2013: Molecular mapping of stripe rust resistance gene YrSN104 in Chinese wheat line Shaannong 104. Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a serious yield-limiting factor for wheat production worldwide. The objective of this study was to identify and map a stripe rust resistance gene in wheat line Shaannong 104 usin...

Van Dolleweerd, C.; Ma, J.K.C., 1999: Identification of two distinct epitopes in a streptococcal surface antigen that are recognised by a monoclonal antibody. Immunology 98(suppl 1): 147, Dec

Hood, E., 2004: RNAi: What's all the noise about gene silencing?. Environmental Health Perspectives 112(4): A224-A229

Presky D.H.; Shevach E.M., 1989: Characterization of a novel phosphatidylinositol pi anchored murine lymphocyte antigen which mediates activation of cd4 positive t cells. FASEB Journal 3(3): A803

Behrman, B.W.; Richards, R.E., 2005: Suspect/foil identification in actual crimes and in the laboratory: a reality monitoring analysis. Four reality monitoring variables were used to discriminate suspect from foil identifications in 183 actual criminal cases. Four hundred sixty-one identification attempts based on five and six-person lineups were analyzed. These identification att...

Gu; Ching, 1987: Comparative studies of electronic and magnetic structures in Y2Fe. Physical Review. B, Condensed Matter 36(16): 8530-8546

Stewart, C.E.; Winer-Muram, H.T.; Jennings, S.Gregory.; Dowdeswell, I.; Meyer, C.A., 2003: Necrotic mass-like upper lobe opacity. Chest 123(1): 277-279

Crusius J.; Pedersen T.F.; Calvert S.E.; Sage D., 1996: Sedimentary Re and Mo enrichments as tracers of low-oxygen conditions; observations and modeling. Eos, Transactions, American Geophysical Union 77(46, Suppl

Chang, H-Ling.; Hsu, Y-Ting.; Kang, C-Yang.; Lee, T-Min., 2014: Nitric oxide down-regulation of carotenoid synthesis and PSII activity in relation to very high light-induced singlet oxygen production and oxidative stress in Chlamydomonas reinhardtii. Nitric oxide (NO) was produced in Chlamydomonas reinhardtii cells 30 min after illumination at a very high light intensity of 3,000 µmol m⁻² s⁻¹ (VHL) followed by singlet oxygen (¹O₂) production, lipid peroxidation, expression of oxidati...