geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Isolation and determination of deoxynivalenol by reversed-phase high-pressure liquid chromatography


, : Isolation and determination of deoxynivalenol by reversed-phase high-pressure liquid chromatography. Pharmaceutical Methods 2(1): 25-29

Deoxynivalenol (DON) is a mycotoxin produced by food contamination. It is a pharmacologically active compound that acts on the serotonin receptor, leading to several neuroendocrine and hematological disorders. In this article we describe a simple, accurate, and sensitive method for the quantification of DON. DON was quantified using a Phenomenex(®) ODS analytical C18 column (150 mm × 46 mm, 5 μm) with a mobile phase composed of mixture of water-acetonitrile-methanol (5:4:1, v/v/v) at a flow rate of 1.5 ml/min and at 254 nm in an ultraviolet (UV) detector The method has been validated with isolated samples of DON and provides a tool for the control of substandard and counterfeit commercial food products.

US$19.90

PMID: 23781426

DOI: 10.4103/2229-4708.81087


Other references

Andzhelov, V.O.; Khvatova, A.V.; Krichevskaya, G.I.; Katargina, L.A.; Shuvegesh, I.; Vekh, M.; Danka, I., 1991: Diagnosis of Toxocara in children with uveitis. Seventeen children aged 2-13 years with various types of uveitis of uncertain aetiology were examined clinically and tested serologically for various chronic infections. Two reacted positively for T. canis in the enzyme immunoassay: one with panuv...

F.G.ang; Chen Zhangming; Jiang Zhenxue, 1995: Study on the capillary sealing ability of cap rocks. Displacement pressure is the most important parameter for evaluating the capillary sealing ability of caprocks. Several methods for calculating it are discussed: the direct displacement method, which reflects actual capillary sealing ability, and...

Bender, L., 1956: Problems in conceptualization and communication in children with developmental alexia. Proceedings of the Annual Meeting of the American Psychopathological Association: 155-176

Monte Secades, R.; Casariego Vales, E.; Mateos Colino, A.; Rigueiro Veloso, T.; Alonso García, P.; Rodríguez Feijoo, A.; Corredoira Sánchez, J., 2000: Clinical profile and prognosis of bacteremia in patients with cirrhosis based on the Child-Pugh classification. The characteristics of 70 cases of bacteremia in cirrhotic patients were studied according to the Child-Pugh classification as severity marker of liver involvement. Factors influencing on prognosis were determined. For a comparative analysis, 1,00...

Sowa, G., 2012: Novel insights into the role of caveolin-2 in cell- and tissue-specific signaling and function. Caveolin-2 is one of the major protein components of cholesterol- and glycosphingolipid-rich flask-shaped invaginations of plasma membrane caveolae. A new body of evidence suggests that caveolin-2 plays an important, and often more direct, role th...

Skandalakis, J.E.; Gray, S.W.; Burns, W.B.; Sangmalee, U.; Sorg, J.L., 1976: Internal and external supravesical hernia. Two cases of supravesical hernia are described. One was a rare posterior internal supravesical hernia discovered only at exploratory laparotomy after exhaustive but fruitless diagnostic procedures. The surgical anatomy of anterior and posterior in...

DiPaolo, J.A.; Donovan, P.; Nelson, R., 1969: Quantitative studies of in vitro transformation by chemical carcinogens. Journal of the National Cancer Institute 42(5): 867-874

Ogonowski, N.; Piro, G.; Pessah, Déborah.; Arreche, N.; Puchulu, B.; Balaszczuk, A.M.; Fellet, A.L., 2016: Thyroid disorders and nitric oxide in cardiovascular adaptation to hypovolemia. This study aimed to investigate whether nitric oxide participates in the cardiovascular function and haemodynamic adaptation to acute haemorrhage in animals with thyroid disorders. Sprague-Dawley rats aged 2months old treated with T3 (hyper, 20μg...

Kondo, T.; Riesz, P., 1991: Effect of ultrasound and ionizing radiation on a sterically hindered cyclic secondary amine: an ESR study. The possible use of 2,2,6,6-tetramethyl-4-piperidone (TMPone) for the detection of singlet oxygen was investigated by gamma radiolysis and sonolysis of oxygen-saturated aqueous solutions. Formation of 2,2,6,6-tetra-methyl-4-piperidone-N-oxyl (TAN)...

Rozenfel'd, A.S., 1969: Caclculation of standardized indices of disability by Carridge's method. Sovetskoe Zdravookhranenie 28(4): 36-39