+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Lactivibrio alcoholicus gen. nov., sp. nov., an anaerobic, mesophilic, lactate-, alcohol-, carbohydrate- and amino-acid-degrading bacterium in the phylum Synergistetes

, : Lactivibrio alcoholicus gen. nov., sp. nov., an anaerobic, mesophilic, lactate-, alcohol-, carbohydrate- and amino-acid-degrading bacterium in the phylum Synergistetes. International Journal of Systematic and Evolutionary Microbiology 64(Pt 6): 2137-2145

A mesophilic, obligately anaerobic, lactate-, alcohol-, carbohydrate- and amino-acid- degrading bacterium, designated strain 7WAY-8-7(T), was isolated from an upflow anaerobic sludge blanket reactor treating high-strength organic wastewater from isomerized sugar production processes. Cells of strain 7WAY-8-7(T) were motile, curved rods (0.7-1.0×5.0-8.0 µm). Spore formation was not observed. The strain grew optimally at 37 °C (range for growth was 25-40 °C) and pH 7.0 (pH 6.0-7.5), and could grow fermentatively on yeast extract, glucose, ribose, xylose, malate, tryptone, pyruvate, fumarate, Casamino acids, serine and cysteine. The main end-products of glucose fermentation were acetate and hydrogen. In co-culture with the hydrogenotrophic methanogen Methanospirillum hungatei DSM 864(T), strain 7WAY-8-7(T) could utilize lactate, glycerol, ethanol, 1-propanol, 1-butanol, L-glutamate, alanine, leucine, isoleucine, valine, histidine, asparagine, glutamine, arginine, lysine, threonine, 2-oxoglutarate, aspartate and methionine. A Stickland reaction was not observed with some pairs of amino acids. Yeast extract was required for growth. Nitrate, sulfate, thiosulfate, elemental sulfur, sulfite and Fe (III) were not used as terminal electron acceptors. The G+C content of the genomic DNA was 61.4 mol%. 16S rRNA gene sequence analysis revealed that the isolate belongs to the uncultured environmental clone clade (called 'PD-UASB-13' in the Greengenes database) in the bacterial phylum Synergistetes, showing less than 90% sequence similarity with closely related described species such as Aminivibrio pyruvatiphilus and Aminobacterium colombiense (89.7% and 88.7%, respectively). The major cellular fatty acids were iso-C(13 : 0), iso-C(15 : 0), anteiso-C(15 : 0), C(18 : 1), C(19 : 1), C(20 : 1) and C(21 : 1). A novel genus and species, Lactivibrio alcoholicus gen. nov., sp. nov. is proposed to accommodate strain 7WAY-8-7(T) ( = JCM 17151(T) = DSM 24196(T) = CGMCC 1.5159(T)).


PMID: 24676730

DOI: 10.1099/ijs.0.060681-0

Other references

Pokrovskaya, I.V., 1966: On the aetiology of the nest-building processes in young sparrows of the Leningrad district. Trudy Pribaltiiskoi Ornitologicheskoi Konferentsii, 6: 119-121

Cui, K.L.; Wang, Z.G.; Li, M.X.; Wang, M.S.; Hong, N.; He, G.M., 1992: Distinguishing Anopheles dirus of Hainan Province by gas chromatography of cuticular hydrocarbons. In order to identify members of the A. dirus complex in Hainan Province, China, a qualitative analysis of the cuticular saturated n-paraffins of A. dirus, collected from various locations in the province in different months, was performed by gas c...

Karp, D.S.; Gennet, S.; Kilonzo, C.; Partyka, M.; Chaumont, N.; Atwill, E.R.; Kremen, C., 2015: Comanaging fresh produce for nature conservation and food safety. In 2006, a deadly Escherichia coli O157:H7 outbreak in bagged spinach was traced to California's Central Coast region, where >70% of the salad vegetables sold in the United States are produced. Although no definitive cause for the outbreak...

Kocsis, J.J.; Harkaway, S.; Santoyo, M.C.; Snyder, R., 1968: Dimethyl sulfoxide: interactions with aromatic hydrocarbons. Dimethyl sulfoxide (DMSO) enhanced the hypertaurinuria produced by benzene, chlorobenzene, and toluene in rats. Undiluted DMSO was more effective than DMSO diluted with water in potentiating the toxicity of benzene in both rats and mice. Supernata...

Anonymous, 1996: Porfimer photodynamic therapy introduced for advanced esophageal cancer. American Journal of Health-System Pharmacy 53(24): 2940-2940

Formicola, A.J., 1995: The relationship between dental and medical education: a perspective for the 1990s. Journal of Dental Education 59(5): 540-545

Katchuik, R., 1992: Abomasal disease in young beef calves: Surgical findings and management factors. A retrospective study was performed on 46 abomasotomies in beef calves aged 1-6 weeks. Clinical abomasal disease in the herds under investigation had an average incidence of 1.0% for the year of the study. The range in incidence among herds was 0....

Robinson, C.; Castaner, J., 1995: Technetium bicisate SPECT imaging agent. Drugs Of The Future. 20(3): 251-255

Srivastava, A.; Chandel, N.; Mehta, N., 2017: Iso-conversional kinetic analysis of quaternary glass re-crystallization. Iso-conversional kinetic analysis is popular in scientific community for analyzing solid-state reactions (e.g., glass/amorphous and amorphous/crystal phase transformations, re-crystallization etc). It is a recognized significant tool to achieve us...

Hiebert, C.A., 1959: The carcinogenic action of methylcholanthrene on mouse skin in organ culture. Alternate squares of fetal mouse skin maintained in organ culture were treated up to 2 weeks with surface applications of 20-methylcholanthrene in amounts varying from 0.4 to 400 [mu][mu]g. Minimal exposure of the skin to the carcinogen resulted i...