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Limited role of secreted aspartyl proteinases Sap1 to Sap6 in Candida albicans virulence and host immune response in murine hematogenously disseminated candidiasis


, : Limited role of secreted aspartyl proteinases Sap1 to Sap6 in Candida albicans virulence and host immune response in murine hematogenously disseminated candidiasis. Infection and Immunity 78(11): 4839-4849

Candida albicans secreted aspartyl proteinases (Saps) are considered virulence-associated factors. Several members of the Sap family were claimed to play a significant role in the progression of candidiasis established by the hematogenous route. This assumption was based on the observed attenuated virulence of sap-null mutant strains. However, the exclusive contribution of SAP genes to their attenuated phenotype was not unequivocally confirmed, as the Ura status of these mutant strains could also have contributed to the attenuation. In this study, we have reassessed the importance of SAP1 to SAP6 in a murine model of hematogenously disseminated candidiasis using sap-null mutant strains not affected in their URA3 gene expression and compared their virulence phenotypes with those of Ura-blaster sap mutants. The median survival time of BALB/c mice intravenously infected with a mutant strain lacking SAP1 to SAP3 was equivalent to that of mice infected with wild-type strain SC5314, while those infected with mutant strains lacking SAP5 showed slightly extended survival times. Nevertheless, no differences could be observed between the wild type and a Δsap456 mutant in their abilities to invade mouse kidneys. Likewise, a deficiency in SAP4 to SAP6 had no noticeable impact on the immune response elicited in the spleens and kidneys of C. albicans-infected mice. These results contrast with the behavior of equivalent Ura-blaster mutants, which presented a significant reduction in virulence. Our results suggest that Sap1 to Sap6 do not play a significant role in C. albicans virulence in a murine model of hematogenously disseminated candidiasis and that, in this model, Sap1 to Sap3 are not necessary for successful C. albicans infection.

US$19.90

PMID: 20679440

DOI: 10.1128/IAI.00248-10


Other references

Saadi, A.T.; Weir, D.M.; Poxton, I.R.; Stewart, J.; Essery, S.D.; Blackwell, C.C.; Raza, M.W.; Busuttil, A., 1994: Isolation of an adhesin from Staphylococcus aureus that binds Lewis a blood group antigen and its relevance to sudden infant death syndrome. A 67 kDa protein was isolated from cell membrane preparations of Staphylococcus aureus (NCTC 10655) by affinity adsorption with synthetic Lewis a antigen conjugated to Synsorb beads. Pre-treatment of buccal epithelial cells expressing Lewis a with...

Oliveira, M.A.S.; Freitas Fialho, J. de; Alves, R.T.; Oliveira, J.N.S.; Gomes, A.C., 2001: Population dynamics of lace bug (Vatiga illudens) on cassava crop in Distrito Federal. Investigations were conducted in Distrito Federal, Brzail, during 1994-97 on 4 cassava varieties. Numbers of nymphs and adult insects were counted, and damage on leaves investigated. Results showed that sweet varieties (Mantiqueira and Jacana) wer...

Lia B.; Chalupa L.M., 1988: Prenatal development of regional specialization in the primate retina. Investigative Ophthalmology & Visual Science 29(ABSTR ISSUE): 378

Ran, S.; Werner, B.; Gluzman, Y.; Eisinger, M., 1995: Regulation of DNA synthesis in bovine aortic endothelial cells. FASEB Journal 9(3): A316

Makrynikola, V.; Bianchi, A.; Bradstock, K.; Gottlieb, D.; Hewson, J., 1994: Migration of acute lymphoblastic leukemia cells into human bone marrow stroma. Most cases of acute lymphoblastic leukemia (ALL) arise from malignant transformation of B-cell precursors in the bone marrow. Recent studies have shown that normal and leukemic B-cell precursors bind to bone marrow stromal cells through the beta-1...

Craven S.E., 1992: Altered colonizing ability for the ceca of broiler chicks by lipopolysaccharide defective mutants of salmonella california and salmonella typhimurium. Poultry Science 71(SUPPL 1): 88

Stjernstedt, R., 1984: First record of the barred long-tailed cuckoo Cercococcyx montanus in Zambia. Bulletin Zambian Ornithological Society, 16: 18-20

Johnson, D.L.; Wenner, A.M., 1970: Recruitment efficiency in honeybees: studies on the role of olfaction. J. apic. Res.: 9: 13-18

Meinzer, F.C.; McCulloh, K.A., 2013: Xylem recovery from drought-induced embolism: where is the hydraulic point of no return?. Tree Physiology 33(4): 331-334

Heinz, A.; Hoffman, J.F., 1990: Membrane sidedness and the interaction of H+ and K+ on Ca2(+)-activated K+ transport in human red blood cells. The sided effects of H+ on Ca2(+)-stimulated K+ transport (the Gardos channel) were studied in human red blood cells. Cells were loaded with Ca2+ during energy depletion with the internal pH adjusted to desired levels prior to treatment with the a...