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Lipopolysaccharide induces aberrant hypermethylation of Hic-1 in mouse embryonic fibroblasts lacking p53 gene


, : Lipopolysaccharide induces aberrant hypermethylation of Hic-1 in mouse embryonic fibroblasts lacking p53 gene. Anticancer Research 28(4b): 2101-2108

The present study asked whether continuous administration with lipopolysaccharide (LPS), a potent inflammatory agent, induces aberrant methylation in the promoter region of tumor suppressor genes and p53 and/or inducible nitric oxide synthase (iNOS) genes involved in its aberrant methylation. Mouse embryonic fibroblasts (MEFs) were prepared from mice harboring four different genotypes (p53+/+iNOS+/+, p53++iNOS-/-, p53-/-iNOS+/+ and p53-/-iNOS-/-). The MEFs were immortalized by 3T3 procedure and continuously cultured under a medium containing LPS or LPS plus interferon (IFN)-gamma during 40 passages. The methylation status in the CpG site of hypermethylated in cancer-1 (Hic-1) exon la and p16 promoter region was monitored using bisulfite-sequencing methods. LPS and LPS plus IFN-gamma induced de novo methylation in the CpG sites of the Hic-1 gene. This site was methylated only in p53-/- MEFs, and the mRNA expression of Hic-1 decreased in p53-/- MEFs compared to p53+/+ MEFs. The methylation patterns of Hic-1, however, were not affected by iNOS gene status. The promoter region of p16 was methylated by increasing the passage, even under the control medium, with LPS administration promoting methylation, particularly in MEFs lacking the iNOS gene. However, the methylation pattern was not significantly different between the p53 genotypes. Our preliminary study suggests that LPS induces de novo methylation in the CpG site in MEFs. For the Hic-1 gene, but not p16, the p53 gene might protect against aberrant methylation. The iNOS gene might not be involved in methylation of the Hic-1 gene, whereas the promoter region of p16 could be prone to methylation in MEFs lacking the iNOS gene.

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PMID: 18751382


Other references

Tai, H.Y.; McHenry, L.; Fritz, P.J.; Furtek, D.B., 1991: Nucleic acid sequence of a 21 kDa cocoa seed protein with homology to the soybean trypsin inhibitor (Kunitz) family of protease inhibitors. Of 20 000 clones of a cDNA library constructed in lambda gt10 from cocoa seed 130 days after pollination, 11% were positive when probed with synthetic oligonucleotides derived from residues 1-14 of the N-terminal amino sequence of the 21 kDa prote...

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Clark, J.; Yates, F.S., 1971: Heterocyclic studies. Part 23. 5-Trifluoromethylpyrimido(5,4-e)-as-triazines. Journal of the Chemical Society. Perkin Transactions 1 13: 2475-2479

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Selby, P.B., 1978: Induction of dominant mutations that cause skeletal malformations in mice. A new approach for estimating genetic risk to humans from radiation is based upon an analysis of the frequency of induction of dominant mutations that cause skeletal abnormalities in mice. The main goal of this work is to improve estimates of the...

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