+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Liquid chromatography-tandem mass spectrometry of porphyrins and porphyrinogens in biological materials: separation and identification of interfering poly(ethylene) glycol by travelling wave ion mobility spectrometry/tandem mass spectrometry

, : Liquid chromatography-tandem mass spectrometry of porphyrins and porphyrinogens in biological materials: separation and identification of interfering poly(ethylene) glycol by travelling wave ion mobility spectrometry/tandem mass spectrometry. Biomedical Chromatography 27(12): 1782-1787

Biological and clinical samples for porphyrin and porphyrinogen analyses by liquid chromatography-tandem mass spectrometry (LC-MS/MS) are often contaminated with poly(ethylene)glycol (PEG), which complicates the interpretation of mass spectra and characterisation of new porphyrin metabolites. Two contaminating PEG molecules (m/z 833 and m/z 835) were completely separated from uroporphyrin I (m/z 831) by travelling wave ion mobility spectrometry and characterised by tandem mass spectrometry. One of the PEG species (m/z 835) also co-eluted with uroporphyrinogen I (m/z 837) and was unresolvable by travelling wave ion mobility spectrometry/MS, therefore contaminating the MS/MS mass spectra owing to isotope distribution. These PEG species, with the [M + H](+) ions at m/z at 833 and/or m/z 835, co-eluted with uroporphyrin I and uroporphyrinogen I by LC-MS/MS and could be wrongly identified as uroporphomethenes.


PMID: 23893773

DOI: 10.1002/bmc.2994

Other references

Laatikainen, T.; Pelkonen, J.; Pesonen, K.; Kajanoja, P., 1982: Effect of sulprostone on concentrations of progesterone, 20 alpha-dihydroprogesterone, estrone, and estradiol in human placental tissue in the first trimester of pregnancy. Placentas were obtained from 35 healthy women whose pregnancy was terminated in the first trimester. Seventeen of them had no prostaglandin medication whereas in 18 women 500 micrograms of sulprostone was administered for cervical softening 12-14...

Kempeneers, J.; Lambert, S.; Joiris, E., 1954: Septicemia from Micrococcus aureus resistant to antibiotics. Revue Medicale de Liege 9(16): 493-495

Wetterberg, L.; Beck-Friis, J.; Kjellman, B.F.; Ljunggren, J.G., 1984: Circadian rhythms in melatonin and cortisol secretion in depression. Advances in Biochemical Psychopharmacology 39: 197-205

Ebadi, S.; Ansari, N.Nakhostin.; Henschke, N.; Naghdi, S.; van Tulder, M.W., 2011: The effect of continuous ultrasound on chronic low back pain: protocol of a randomized controlled trial. Chronic non-specific low-back pain (LBP) is one of the most common and expensive musculoskeletal disorders in industrialized countries. Similar to other countries in the world, LBP is a common health and socioeconomic problem in Iran. One of the m...

Qu, Z.; Steiner, H.; Engström, A.; Bennich, H.; Boman, H.G., 1982: Insect immunity: isolation and structure of cecropins B and D from pupae of the Chinese oak silk moth, Antheraea pernyi. The immune system in the Chinese oak silk moth, Antheraea pernyi, has been compared with that of the Cecropia moth which has been characterized earlier. Antibacterial activity against Escherichia coli was induced in diapausing pupae by injection o...

Van Veldhuisen D.J.; Girbes A.R.J.; Van Den Broek S.A.J.; Lie K.I., 1991: Ibopamine lowers catecholamines during exercise in patients with heart failure. Journal of the American College of Cardiology 17(2 SUPPL A): 353A

Pores, J.H., 1995: Managing risk to reduce costs. Historically, medical malpractice has been the focus of risk management because of the high cost of malpractice insurance premiums and claims. By employing risk control and risk financing in nonclinical as well as clinical areas, however, risk man...

Schügerl, K., 2005: Extraction of primary and secondary metabolites. Several new methods besides the usual organic solvent extraction have been developed over the last few years for the extraction of primary and secondary metabolites. These are: alcohol extraction with various biocompatible solvents, recovery of ca...

Czar, K.A.; Dahlen, E.R.; Bullock, E.E.; Nicholson, B.C., 2011: Psychopathic personality traits in relational aggression among young adults. Psychopathy is a robust predictor of overt physical aggression that may also be relevant to relational aggression (RA). This study was conducted to investigate the utility of psychopathic personality traits in the prediction of RA in a sample of 2...

Blanchet, P., 1978: Paroxysmal vaso motor skin manifestations. Generalized vasomotor skin manifestations may be induced by a physiological reaction (emotional flushing), by a drug (vasodilator drugs, anti-abuse, antidiabetic, sulfonamides), by a discharge of histamine (urticaria, mastocytosis) or by hypersecr...