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MOS1 osmosensor of Metarhizium anisopliae is required for adaptation to insect host hemolymph


, : MOS1 osmosensor of Metarhizium anisopliae is required for adaptation to insect host hemolymph. Eukaryotic Cell 7(2): 302-309

Entomopathogenic fungi such as Metarhizium anisopliae infect insects by direct penetration of the cuticle, after which the fungus adapts to the high osmotic pressure of the hemolymph and multiplies. Here we characterize the M. anisopliae Mos1 gene and demonstrate that it encodes the osmosensor required for this process. MOS1 contains transmembrane regions and a C-terminal Src homology 3 domain similar to those of yeast osmotic adaptor proteins, and homologs of MOS1 are widely distributed in the fungal kingdom. Reverse transcription-PCR demonstrated that Mos1 is up-regulated in insect hemolymph as well as artificial media with high osmotic pressure. Transformants containing an antisense vector directed to the Mos1 mRNA depleted transcript levels by 80%. This produced selective alterations in regulation of genes involved in hyphal body formation, cell membrane stiffness, and generation of intracellular turgor pressure, suggesting that these processes are mediated by MOS1. Consistent with a role in stress responses, transcript depletion of Mos1 increased sensitivity to osmotic and oxidative stresses and to compounds that interfere with cell wall biosynthesis. It also disrupted developmental processes, including formation of appressoria and hyphal bodies. Insect bioassays confirmed that Mos1 knockdown significantly reduces virulence. Overall, our data show that M. anisopliae MOS1 mediates cellular responses to high osmotic pressure and subsequent adaptations to colonize host hemolymph.

US$19.90

PMID: 18055914

DOI: 10.1128/EC.00310-07


Other references

Makohon, N.V.; Aleksieieva, I.M., 1994: Effect of cultured mice hepatocytes on the proliferation of immunocompetent cells. Mice hepatocytes in primary cultures were treated during 2 h with normal rabbit antibodies (0.5 mg/ml), antiliver antibodies (0.5 mg/ml) and CCl4 (5 mM). Functional state of cells was studied according to DNA, RNA and protein synthesis. Cell-free...

Carlsen K H.; Leegaard J.; Larsen S.; Orstavik I., 1988: Nebulized beclomethasone dipropionate in recurrent obstructive episodes after acute bronchiolitis. Forty-four children with recurrent obstructive episodes after acute bronchiolitis in infancy were treated with nebulised beclomethasone dipropionate or placebo for eight weeks in a randomised double-blind study. They were seen monthly for a year a...

Anderson, H.W.; Gottlieb, D., 1952: Plant disease control with antibiotics. This review is limited to publications dealing with antibiotic substances, adequately defined chemically or biologically, which have been used for attempted control of diseases incited by plant-pathogs. The origin, chemical nature, and exptl. resu...

Harwood, L.; Wilson, B.; Goodman, M., 2017: Cannulation Outcomes of the Arteriovenous Fistula for Hemodialysis: A Scoping Review. The arteriovenous fistula (AVF) is the recommended access for most people requiring hemodialysis. Success or failure of cannulation of the AVF greatly impacts the patient's experience. Research suggests that skill development in this area is...

Lucifero, M.T.imarchi, G.F.rruzzi, G., 1978: Finnish Landrace breed and fertility. Informatore zootecnico, 25(1): 17-20

Park J.K., 1977: The effects of cervical eversion on the courses of labor. Journal Of Pusan Medical College: 257-265

Beyers, E., 1963: Control of mineral nutrition of deciduous fruit trees and vines. In view of the fact that most of the orchard and vineyard soils in the Western Cape Province are relatively poor and weakly buffered, excesses and imbalances as well as deficiencies of mineral nutrients are very prevalent. It is most important the...

Bhandari, S.; Wilkinson, A.; Nicholson, A.; Farr, M.J.; Sellars, L., 1996: Angioplasty with stenting versus surgery for renovascular disease. Nephrology Dialysis Transplantation 11(6): A146

Rowe, N.L., 1969: Nonunion of the mandible and maxilla. Journal of Oral Surgery 27(7): 520-529

Kaya, Sçuk.; Köksal, I.; Menteşe, A.; Sönmez, M.; Sümer, Aşegül.; Yıldırım, S.Sönmez.; Yılmaz, Gürdal., 2014: The significance of serum urokinase plasminogen activation receptor (suPAR) in the diagnosis and follow-up of febrile neutropenic patients with hematologic malignancies. The purpose of this study was to investigate serum levels of serum urokinase plasminogen activation receptor (suPAR) during the first week of febrile neutropenia and to demonstrate the significance of this biomarker in the diagnosis and follow-up...