geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Manipulation of the peptide-binding specificity of an RNA in a rational manner by combinations of specificity-altering mutations


, : Manipulation of the peptide-binding specificity of an RNA in a rational manner by combinations of specificity-altering mutations. Nucleic Acids Symposium Series: 13-14

In this study, the potential to manipulate the peptide-binding specificity of an RNA in a rational manner was investigated. First, variants of the Rev-response element (RRE) RNA with different specificities towards the natural binding partner, Rev, and two RRE-binding aptamers, the RSG-1.2 and K1 peptides, were identified. Next, hybrid RRE mutants with combinations of two sets of specificity-altering substitutions were tested for peptide-binding specificity. It was shown that, in most cases, the results of the combination of individual mutations were of an additive nature, therefore providing a way to manipulate the peptide-binding specificity of an RNA in a predictable manner.

US$19.90

PMID: 18776229

DOI: 10.1093/nass/nrn007


Other references

Anonymous, 1993: Psychophysiological Measures in Transport Operations. Workshop proceedings. Cologne, November 1990. Ergonomics 36(9): 989-1140

Ames, R.M., 2017: Using Network Extracted Ontologies to Identify Novel Genes with Roles in Appressorium Development in the Rice Blast Fungus Magnaporthe oryzae. <i>Magnaporthe oryzae</i> is the causal agent of rice blast disease, the most important infection of rice worldwide. Half the world's population depends on rice for its primary caloric intake and, as such, rice blast poses a serio...

Hsuchou, H.; Pan, W.; Kastin, A.J., 2013: Fibroblast growth factor 19 entry into brain. Fibroblast growth factor (FGF)-19, an endocrine FGF protein mainly produced by the ileum, stimulates metabolic activity and alleviates obesity. FGF19 modulates metabolism after either intravenous or intracerebroventricular injection, and its recep...

Abusedera, M.; Alkady, O., 2016: Ultrasound-guided Pleural Effusion Drainage With a Small Catheter Using the Single-step Trocar or Modified Seldinger Technique. Studies have shown that small-catheter pleural effusion drainage is safe and has a lower complication rate. Our objective was to evaluate the outcomes and the safety of the single-step trocar or the modified Seldinger technique. A total of 124 pat...

Sanchez-Nieva, F.M.rcado, M., 1978: Effect of peeling method on the absorption of aqueous bisulfite by green bananas. Journal of agriculture of the University of Puerto Rico: 62 (2) 191-198

Fowler S.C.; Ford K.E.; Gramling S.E.; Nail G.L., 1984: Acute and subchronic effects of neuroleptics on quantitative measures of discriminative motor control in rats. Operant-conditioning methods were used to train rats to reach through an opening in an operant chamber to exert forces on a silent, nearly isometric force-sensing manipulandum. The reinforcement contingency required the rat to hold forelimb force...

Koshino, T.; Takano, S.; Houjo, T.; Sano, Y.; Kudo, K.; Kihara, H.; Kitani, S.I.hi; Takaishi, T.; Hirai, K.; Ito, K.; Morita, Y., 1998: Expression of 5-lipoxygenase and 5-lipoxygenase-activating protein mRNAs in the peripheral blood leukocytes of asthmatics. Leukotrienes are a family of arachidonic acid metabolites with potent biological activities such as bronchoconstriction and leukocyte chemotaxis. Recent evidence has demonstrated that the 5-lipoxygenase (5-LO) and 5-lipoxygenase-activating protein...

Cherdakov, V.V.; Voroshilova, M.K., 1968: Chromatography of ECHO 7 and ECHO 12 viruses and their hemagglutinins on DEAE-cellulose and calcium phosphate. Voprosy Virusologii 13(2): 142-146

Bishop, G.A.; Zhang, Y.; Mclaren, S.E.; Guenther, P.L.; Beaton, S.P.; Peterson, J.E.; Stedman, D.H.; Pierson, W.R.; Knapp, K.T., 1994: Enhancements of remote sensing for vehicle emissions in tunnels. The University of Denver's remote sensing system for vehicle exhaust has been successfully adapted to the measurement of vehicle emissions in a tunnel environment. Two studies conducted at the Fort McHenry Tunnel in Baltimore, MD and the Tusc...

Soriano, M.J.; Guardia, M.D.L., 1984: A comparative study of flame atomic-absorption methods for determination of zinc in serum and blood plasma. Of 7 methods used to estimate zinc in blood 5 were adequate. The methods of Butrimovitz and Purdy (NAR/A 48, 4045), Smith et al. (NAR/A 50, 1698) and Fuwa et al. (NAR 35, 3472) were the most exact and precise.