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Measuring spatiotemporal dynamics of cyclic AMP signaling in real-time using FRET-based biosensors


, : Measuring spatiotemporal dynamics of cyclic AMP signaling in real-time using FRET-based biosensors. Methods in Molecular Biology 746: 297-316

Cyclic AMP governs many fundamental signaling events in eukaryotic cells. Although cAMP signaling has been a major research focus for a long time, recent technological developments are revealing novel aspects of this paradigmatic pathway. In this chapter, we give an overview over current fluorescence resonance energy transfer (FRET)-based sensors for detection of cAMP dynamics, and their application in monitoring local, compartmentalized cAMP signals within living cells. A basic step-by-step protocol is given for conducting a FRET experiment in primary cells with a unimolecular cAMP sensor, which can easily be adapted to a user's specific requirements.

US$19.90

PMID: 21607864

DOI: 10.1007/978-1-61779-126-0_16


Other references

Imamaliev, S.A., 2000: The centenary of the birth of Salar Agarafievich Imamaliev. Zhurnal Mikrobiologii, Epidemiologii, i Immunobiologii: 125-125

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Al'Saud, K., 1988: Megavolt therapy of patients with lumbosacral radiculitis. Vrachebnoe Delo: 103-104

Boysen, M.; Loven, J.O., 1993: Second malignant neoplasms in patients with head and neck squamous cell carcinomas. The incidence of second malignant neoplasms (SMN) was analyzed in 714 patients with squamous cell carcinomas of the oral cavity, oropharynx, hypopharynx and larynx. With a minimum follow-up of 3.5 years and 2,540 person-years at risk 84 SMN (10 sy...

Flora, Cb, 1981: Farm women, farming systems, and agricultural structure: suggestions for scholarship. Rural sociologist 1(6): 383-386

Senft, D., 1993: Getting research to users--a coordinated approach. Agricultural research 41(11): 20-21

Yao Lingkan; Fang Duo, 1998: On the self-organized criticality of non-uniform sands. From the view point of self-organized criticality, this paper investigates an essential distinction between non-uniform and uniform sands. First, flume experiments on sand pile model demonstrate that non-uniform sand system under water exhibits se...

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