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Mechanism of macrophage activation induced by beta-glucan produced from Paenibacillus polymyxa JB115

, : Mechanism of macrophage activation induced by beta-glucan produced from Paenibacillus polymyxa JB115. Biochemical and Biophysical Research Communications 391(3): 1358-1362

Beta-glucans are heterogeneous groups of glucose polymers found in the cell walls of fungi, plants and some bacteria. Our previous report showed that a novel beta-1,3/1,6-glucan produced from Paenibacillus (P.) polymyxa JB115 can induce nitric oxide (NO) production in RAW264.7 cells. In the present study, the beta-glucan significantly increased luciferase activity in cells transfected with NFkappaB or AP1, but not STAT1, reporter vector DNA, which contain their binding promoter site. All specific NFkappaB and MAPKs pathway inhibitors (pyrrolidine dithiocarbamate, AG490, U0126, SB203580 and SP600125) remarkably attenuated NO production induced by the beta-glucan. Furthermore, Western blot analysis revealed that the stimulation of Raw264.7 cells by beta-glucan induced phosphorylation of IkappaB and the consequent translocation of NFkappaB into the nucleus. Meanwhile, phosphorylation of ERK1/2, JNK/SAPK and p38 MAPKs in cytoplasm were also confirmed. All these results indicated that beta-glucan from P. polymyxa JB115 activates macrophages through MAPKs and NFkappaB signaling pathway.


PMID: 20026063

DOI: 10.1016/j.bbrc.2009.12.064

Other references

McClain James S.; Moores E.M.; Chin Chen, 1986: Seismic velocities and the penetration of water into the crust at mid-ocean ridges. Eos, Transactions, American Geophysical Union: 4, Pages 1244. 1986.

Morson B.C., 1983: Histology and behavior of endocrine cell carcinoid tumors of the rectum. Pathology Research & Practice: 153

Grant, M.A.; Shanmugasundaram, K.; Rigby, A.C., 2007: Conotoxin therapeutics: a pipeline for success?. The pharmacopoeia of conotoxins from the marine snail Conus has evolved with time, providing a myriad of molecular scaffolds on which critical, molecular pharmacophoric descriptors, responsible for mediating conotoxin receptor-target specificity a...

Sato, Y.; Iwamatsu, R.; Takahashi, M., 1958: Examination of the freshness of marketing fish in summer in Sendai. The volatile basic and amine-N of the fish sold at a store in Sendai, August, 1957, were determined by Conway's microdiffusion method. Comparing the values with those in fish of May and June 1957, the quality of marketing fish was better in s...

Anonymous, 1969: Demonstrations. C. Snyder in ' Trials, errors and successes in agribusiness education at Purdue' maintains that the undergraduate curriculum should include: (1) a broad and liberal education in the social and natural sciences; (2) adequate professional...

Pot, G.K.; Majsak-Newman, G.; Geelen, A.; Harvey, L.J.; Nagengast, F.M.; Witteman, B.J.M.; van de Meeberg, P.C.; Timmer, R.; Tan, A.; Wahab, P.J.; Hart, A.R.; Williams, M.P.; Przybylska-Phillips, K.; Dainty, J.R.; Schaafsma, G.; Kampman, E.; Lund, E.K.; ter Telgte, Y.; van Wijngaarden, J.; Brattinga, C.; Smits, M.; Kok, A.; van Steenbergen, L.; Jöris, M.; Bellmann, S.; Visser, M.; van Heijningen, E-Mariette.; Mieloo, C.; Stol, J.; Walters, M.; Enneman, A.; Hilbers, A.Maria.; van der Struijs, B., 2009: Fish consumption and markers of colorectal cancer risk: a multicenter randomized controlled trial. Diet is a major factor in the etiology of colorectal cancer, with high fish consumption possibly decreasing colorectal cancer risk, as was shown in several observational studies. To date, no intervention trials have examined the possible beneficia...

Kaye, J.; Solomon, A., 1964: The Use Of Dulcolax In Propylene Glycol In The Radiological Investigation Of The Colon. The use of liquid Dulcolax (bisacodyl) which is non-toxic, and acts selectively on the colon, is recommended in place of tannic acid for barium enema examinations. The chemistry and pharmacology of Dulcolax is mentioned. A survey of 150 patients s...

Burns, J.R.; Preus, Søren.; Singleton, D.G.; Stulz, E., 2013: A DNA based five-state switch with programmed reversibility. A programmable switch based on a DNA hairpin loop is functionalised with a rigid or flexible porphyrin or FAM and TAMRA FRET pair, which provides insight into the restructuring of the hairpin as well as porphyrin-porphyrin coupling. The switch con...

Largeron, J.P.; Galonnier, F.; Védrine, N.; Alfidja, A.; Boyer, L.; Pereira, B.; Boiteux, J.P.; Kemeny, J.L.; Guy, L., 2014: Multiparametric 3T MRI in the routine staging of prostate cancer. To analyse the detection ability of a multiparametric 3T MRI with phased-array coil in comparison with the pathological data provided by the prostatectomy specimens. Prospective study of 30 months, including 74 patients for whom a diagnosis of pro...

Strunge, P.; Trostmann, A.F., 1972: The lipoprotein pattern in a Danish family. Children and adolescents. Acta Medica Scandinavica 192(4): 331-335