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Mechanism of mitochondrial membrane permeabilization during apoptosis under photofrin-mediated photodynamic therapy

, : Mechanism of mitochondrial membrane permeabilization during apoptosis under photofrin-mediated photodynamic therapy. Journal of X-Ray Science and Technology 20(3): 363-372

Photofrin-mediated photodynamic therapy (PF-PDT) can induce cell apoptosis via the mitochondria/caspase-3 pathway. Here, we further investigate the mechanism involved in the mitochondrial apoptotic process induced by PF-PDT. A high-level intracellular reactive oxygen species (ROS) generation in mitochondria, mitochondrial swelling, and dissipation of mitochondrial transmembrane potential were observed immediately after irradiation, indicating that mitochondria were the major ROS generation sites and also the first oxidative damage sites after PF-PDT treatment. For mitochondrial permeability detection, the decrease of calcein fluorescence emission intensity and release of cytochrome c were observed immediately after PF-PDT treatment, indicating the occurrence of mitochondrial inner membrane permeabilization (MIMP) and the mitochondrial outer membrane permeabilization (MOMP). However, cytochrome c release was not prevented by cyclosporine (CsA), a specific inhibitor of mitochondrial permeability transition (MPT). Taken together, these results demonstrated that PF-PDT caused simultaneous onset of MIMP and MOMP immediately after the treatment, and MOMP was independent of the MPT. Besides, inducible mitochondrial ROS generation played key roles in PF-PDT-induced cell apoptosis. This study will be benefit for understanding the mechanism involved in the initial mitochondrial oxidative damage by PF-PDT.


PMID: 22948357

DOI: 10.3233/XST-2012-0344

Other references

Liner, E.A., 1955: A herpetological consideration of the Bayou Tortue region of Lafayette Parish, Louisiana. Proceedings of the Louisiana Academy of Sciences, 18: 39-42

Barker, J.S.; Starmer, W.T.; Vacek, D.C., 1987: Analysis of spatial and temporal variation in the community structure of yeasts associated with decayingOpuntia cactus. The microbial structure within, between, and over time in decaying cladodes of the common prickly pearOpuntia stricta was studied at each of two separate localities. In general, the effective number of yeast species and yeast species diversity inc...

Samuel, J.; Nind, M.; Volans, A.; Scriven, I., 2008: An evaluation of Intensive Interaction in community living settings for adults with profound intellectual disabilities. Intensive Interaction is an approach to enhancing the communication and social abilities of people with profound intellectual disabilities using principles from caregiver-infant interaction. Use of Intensive Interaction by novice practitioners wit...

Amiri, A.; Firoozeh, F.; Moniri, R.; Zibaei, M., 2016: Prevalence of CTX-M-Type and PER Extended-Spectrum β-Lactamases Among Klebsiella spp. Isolated From Clinical Specimens in the Teaching Hospital of Kashan, Iran. Extended-spectrum β-lactamases (ESBLs) is one of the most important mechanisms of resistance to β-lactams especially among Enterobacteriaceae family including Klebsiella spp. Different types of extended-spectrum β-lactamases including CTX-M-typ...

Peterson, D.W.; Shneour, E.A.; Peek, N.F.; Gaffey, H.W., 1953: Dietary constituents affecting plasma and liver cholesterol in cholesterol-fed chicks. Hypercholesteremia and extreme liver cholesterosis were prevented in chicks fed high (1%) cholesterol diets by the inclusion in the diet of mixed soy sterols, mixed sitosterols, beta-sitosterol, ergosterol or stigmasterol. When cholesterol was fed...

Hao, L.; Leng, J.; Xiao, R.; Kingsley, T.; Li, X.; Tu, Z.; Yang, X.; Deng, X.; Xiong, M.; Xiong, J.; Zhang, Q., 2017: Bioinformatics analysis of the prognostic value of Tripartite Motif 28 in breast cancer. Tripartite motif containing 28 (TRIM28) is a transcriptional regulator acting as an essential corepressor for Krüppel-associated box zinc finger domain-containing proteins in multiple tissue and cell types. An increasing number of studies have in...

Caffaratto, T.M., 1958: Historicl obstetrics and fantastic obstetrics. Minerva Ginecologica 10(17): 699-702

Ostapov, V.; Fesenko, O.; Chornoostrovets'-, I.; Mel'-Nychuk, A., 1978: A system of soil tillate in an i. Zroshuvane zemlerobstvo: 3) 9-15

Williams, J., 2012: The recognition and management of isolated cleft palate. Cleft palate in the absence of a cleft lip (i.e. isolated cleft palate) causes upset for parents whenever it is diagnosed; however, delayed diagnosis at over 24 hours of age can cause increased distress due to feeding difficulties and fear of what...

Minaker, K.; Little, H., 1973: Painful feet in rheumatoid arthritis. Pain in the feet is an important diagnostic feature and a major management problem of rheumatoid arthritis. Of 50 hospitalized patients, 28% recalled painful feet as the sole presenting symptom of their disease.RHEUMATOID DISEASE COMMONLY AFFECTS...