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Mechanisms involved in nicotinic acetylcholine receptor-induced neurotransmitter release from sympathetic nerve terminals in the mouse vas deferens


, : Mechanisms involved in nicotinic acetylcholine receptor-induced neurotransmitter release from sympathetic nerve terminals in the mouse vas deferens. Plos One 6(12): E29209-E29209

Prejunctional nicotinic acetylcholine receptors (nAChRs) amplify postganglionic sympathetic neurotransmission, and there are indications that intraterminal Ca(2+) stores might be involved. However, the mechanisms by which nAChR activation stimulates neurotransmitter release at such junctions is unknown. Rapid local delivery (picospritzing) of the nAChR agonist epibatidine was combined with intracellular sharp microelectrode recording to monitor spontaneous and field-stimulation-evoked neurotransmitter release from sympathetic nerve terminals in the mouse isolated vas deferens. Locally applied epibatidine (1 µM) produced 'epibatidine-induced depolarisations' (EIDs) that were similar in shape to spontaneous excitatory junction potentials (SEJPs) and were abolished by nonselective nAChR antagonists and the purinergic desensitizing agonist α,β-methylene ATP. The amplitude distribution of EIDs was only slightly shifted towards lower amplitudes by the selective α7 nAChR antagonists α-bungarotoxin and methyllcaconitine, the voltage-gated Na(+) channel blocker tetrodotoxin or by blocking voltage-gated Ca(2+) channels with Cd(2+). Lowering the extracellular Ca(2+) concentration reduced the frequency of EIDs by 69%, but more surprisingly, the Ca(2+)-induced Ca(2+) release blocker ryanodine greatly decreased the amplitude (by 41%) and the frequency of EIDs by 36%. Ryanodine had no effect on electrically-evoked neurotransmitter release, paired-pulse facilitation, SEJP frequency, SEJP amplitude or SEJP amplitude distribution. These results show that activation of non-α7 nAChRs on sympathetic postganglionic nerve terminals induces high-amplitude junctional potentials that are argued to represent multipacketed neurotransmitter release synchronized by intraterminal Ca(2+)-induced Ca(2+) release, triggered by Ca(2+) influx directly through the nAChR. This nAChR-induced neurotransmitter release can be targeted pharmacologically without affecting spontaneous or electrically-evoked neurotransmitter release.

US$19.90

PMID: 22216213

DOI: 10.1371/journal.pone.0029209


Other references

Reichert, L.E.; Bhalla, V.K., 1974: Development of a radioligand tissue receptor assay for human follicle-stimulating hormone. Endocrinology 94(2): 483-491

Fernando, S.; Hill, C.M.; Walker, R., 1993: A randomised double blind comparative study of low level laser therapy following surgical extraction of lower third molar teeth. A randomised, double blind comparative study was undertaken to assess the efficacy of low level laser therapy in the reduction of postoperative pain and swelling in patients undergoing the extraction of bilaterally impacted mandibular third molar...

Mcmillen Jackson A.L.; Liehr J.G., 1991: Microsome mediated dna adduct formation by steroid estrogens. Proceedings of the American Association for Cancer Research Annual Meeting 32: 111

Jirmanova, I., 1993: Giant axonopathy in streptozotocin diabetes of rats. The ultrastructure of peripheral sensory nerves was investigated in adult Wistar rats suffering from experimental diabetes mellitus 6 and 10 weeks after the injection of streptozotocin. Giant axons were seen in sections from the nerves of streptoz...

Milosevic, B.; Blagojevic, M., 1954: Surgical treatment of intraocular cysticercosis. Srpski Arhiv Za Celokupno Lekarstvo 82(3): 364-371

Trépanier, P.; Tremblay, C.; Ruest, A., 2014: Methicillin-resistant Staphylococcus aureus colonization among medical residents. Medical residents may be at risk of becoming colonized by methicillin-resistant Staphylococcus aureus (MRSA) during their training. The occupational risk of this specific population is unknown. Furthermore, there are no data regarding MRSA coloniz...

Cao, J-qing.; Zhang, C.; Feng, S-wei.; Yang, J.; Li, Z.; Zhang, M.; Li, S-ying.; Sun, X-fang.; Wang, Y-yun.; Zheng, M-ying.; Kong, J., 2011: Study of dystrophin gene non-deletion/duplication mutations causing Becker muscular dystrophy. To identify potential mutations in patients featuring Becker muscular dystrophy (BMD) and to enhance the understanding of non-deletion/duplication mutations of the dystrophin gene causing BMD. Clinical data of two patients affected with BMD were c...

Zhang, H.; Li, J.; Barrington, R.A.; Liang, G.; Qin, G.; Liu, D-Xu., 2007: An anti-endotoxin peptide that generates from the amino-terminal domain of complement regulatory protein C1 inhibitor. C1 inhibitor (C1INH), a complement regulatory protein, prevents endotoxin shock via a direct interaction of the amino-terminal domain with gram-negative bacterial lipopolysaccharide (LPS). Importantly, the cleaved, inactive C1INH still is an anti...

Brainard, S.C., 1995: How open heart surgery came to Hawaii. The evolution of open heart surgery had its beginnings in 1938 with the closing of a patent ductus arteriosus. In 1954 Hawaii began to perform right heart catheterizations and a bubble oxygenator was made locally out of plastic food tubing and a S...

Kelly, R.L., 1948: General principles in dermatological diagnosis. Kentucky Medical Journal 46(8): 321-323