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Mechanisms of bradykinin-induced contraction in human fetal lung fibroblasts


, : Mechanisms of bradykinin-induced contraction in human fetal lung fibroblasts. European Respiratory Journal 36(3): 655-664

Bradykinin (BK) induces fibroblast contraction but the structural changes and intracellular mechanisms involved have not been completely explored. We stimulated HFL-1 fibroblasts with BK to assess: 1) fibroblast contractility; 2) the role of α-smooth muscle actin (SMA) in contraction by small interfering RNA (siRNA); 3) α-SMA protein expression; 4) α-SMA and F-actin structure; 5) intracellular calcium concentration ([Ca(2+)](i)); and 6) phosphorylated myosin light-chain (pMLC) and MLC kinase (MLCK) expression. BK triggered concentration- and time-dependent fibroblast gel contraction in conjunction with α-SMA over expression, but not in α-SMA-siRNA-treated cells. BK also increased α-SMA(+) and F-actin(+) cell number and stress fibre polymerisation (detectable at 5-60 min). These BK-induced changes were associated with an increase in [Ca(2+)](i), which peaked within 15 s, and activation of pMLC, which was detectable at 5-60 min. No MLCK content modification was observed. The different manifestations of the BK-induced fibroblast activation were downregulated at different levels (25-100%) by HOE140, a specific BK B2 receptor (B2R) antagonist and by the Ca(2+) chelator, EGTA. Thus, BK-induced fibroblast contraction, associated with differentiation into α-SMA(+) myofibroblasts, is mediated through the activation of the B2R and involves the Ca(2+)/calmodulin pMLC-dependent pathway.

US$19.90

PMID: 20351037

DOI: 10.1183/09031936.00112209


Other references

Calladine, C.R.; Drew, H.R., 1984: A base-centred explanation of the B-to-A transition in DNA. In the traditional view, the bistable feature responsible for the switch between the B and A forms of DNA was the sugar-phosphate backbone. Several recent assays of the sequence-dependent structure of DNA are not compatible with that hypothesis. H...

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Bazin, S.; Delaunay, A., 1970: Mucopolysaccharides in inflammatory foci with rapid or slow evolution. Exposes Annuels de Biochimie Medicale 30: 173-187

Gac, A., 1976: Reducing energy losses in greenhouses. Plasticulture 31 35-42

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Brown, A.M.; Kaplan, L.M.; Brown, M.E., 1960: Phenol-induced histological skin changes: hazards, technique, and uses. British Journal of Plastic Surgery 13: 158-169

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