+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Mechanisms of contact-mediated killing of yeast cells on dry metallic copper surfaces

, : Mechanisms of contact-mediated killing of yeast cells on dry metallic copper surfaces. Applied and Environmental Microbiology 77(2): 416-426

Surfaces made of copper or its alloys have strong antimicrobial properties against a wide variety of microorganisms. However, the molecular mode of action responsible for the antimicrobial efficacy of metallic copper is not known. Here, we show that dry copper surfaces inactivate Candida albicans and Saccharomyces cerevisiae within minutes in a process called contact-mediated killing. Cellular copper ion homeostasis systems influenced the kinetics of contact-mediated killing in both organisms. Deregulated copper ion uptake through a hyperactive S. cerevisiae Ctr1p (ScCtr1p) copper uptake transporter in Saccharomyces resulted in faster inactivation of mutant cells than of wild-type cells. Similarly, lack of the C. albicans Crp1p (CaCrp1p) copper-efflux P-type ATPase or the metallothionein CaCup1p caused more-rapid killing of Candida mutant cells than of wild-type cells. Candida and Saccharomyces took up large quantities of copper ions as soon as they were in contact with copper surfaces, as indicated by inductively coupled plasma mass spectroscopy (ICP-MS) analysis and by the intracellular copper ion-reporting dye coppersensor-1. Exposure to metallic copper did not cause lethality through genotoxicity, deleterious action on a cell's genetic material, as indicated by a mutation assay with Saccharomyces. Instead, toxicity mediated by metallic copper surfaces targeted membranes in both yeast species. With the use of Live/Dead staining, onset of rapid and extensive cytoplasmic membrane damage was observed in cells from copper surfaces. Fluorescence microscopy using the indicator dye DiSBaC(2)(3) indicated that cell membranes were depolarized. Also, during contact-mediated killing, vacuoles first became enlarged and then disappeared from the cells. Lastly, in metallic copper-stressed yeasts, oxidative stress in the cytoplasm and in mitochondria was elevated.


PMID: 21097600

DOI: 10.1128/AEM.01704-10

Other references

Medway, L., 1970: The biology of swiftlets from southeast asia. Journal fuer Ornithologie 111(2): 196-205

Sinha, U.S.P.; Sinha, A.K., 1993: Studies on free amino acids, proteins, carbohydrates and phosphorus compounds in the tissue extracts of moths of temperate tasar silkworm, Antheraea proylei J. In laboratory studies, 17 amino acids were identified in both males and females of the silkworm Antheraea proylei, and in general, the concn of almost all (with the exception of leucine and isoleucine), were higher in males. There was no significa...; Wang Ming; Kong FanYao; Yin PeiYun, 2002: Ultrastructural effect of maduramisin on Eimeria tenella. Ultrastructural changes of Eimeria tenella from chickens treated with maduramisin-supplemented (0.0005%) food are described. The meronts become small, and the number of merozoites decreased. The microtubules between them become swollen. The merozo...

Takemi, T., 1966: The goverment and medical care progress. Nihon Ishikai Zasshi. Journal of the Japan Medical Association 56(7): 714-720

Bünermann, O.; Mudrich, M.; Weidemüller, M.; Stienkemeier, F., 2004: Spectroscopy of Cs attached to helium nanodroplets. Cesium oligomers are formed on helium nanodroplets which are doped with one or a few Cs atoms. The monomer absorption of the first electronic p<--s transition upon laser excitation is probed. Spectra employing laser-induced fluorescence, beam d...

Attari, M.; Jamaloo, F.; Shadvar, S.; Fakhraei, N.; Dehpour, A.Reza., 2016: Effect of Withania somnifera Dunal Root Extract on Behavioral Despair Model in Mice: a Possible Role for Nitric Oxide. Withania somnifera (WS) possess anti-inflammatory and antioxidant properties. WS preparations have a potential therapeutic role in the central nervous system (CNS) related disorders in animal models. In this study, the possible protective effect o...

Harris, M.; Freeman, T.; Rohfritsch, O.A.derson, K.; Payne, S.; Moore, J., 2006: Virulent Hessian Fly (Diptera: Cecidomyiidae) Larvae Induce a Nutritive Tissue During Compatible Interactions with Wheat. The compatible interaction between virulent Hessian fly, Mayetiola destructor (Say), larvae and susceptible wheat, Triticum aestivum L., plants was investigated at the light microscope and ultrastructural levels. During the first day of larval att...

Chiou, S-Ti., 2016: Transformar nuestros entornos: haremos que suceda en todas partes. Global Health Promotion 23(1): 109-111

Premont, R.T.; Chen, J.; Ma, H.W.; Ponnapalli, M.; Iyengar, R., 1992: Two members of a widely expressed subfamily of hormone-stimulated adenylyl cyclases. cDNA encoding a hormone- and guanine nucleotide-stimulated adenylyl cyclase [ATP pyrophosphate-lyase (cyclizing), EC] (type 6) from rat liver and kidney has been cloned and expressed. This enzyme is stimulated by forskolin, guanosine 5&apo...

Barnett, S., 1985: New Zealand in the wild. An illustrated A-Z of native and introduced birds, mammals, reptiles and amphibians. Unknown