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Methylglyoxal augments angiotensin II-induced contraction in rat isolated carotid artery

, : Methylglyoxal augments angiotensin II-induced contraction in rat isolated carotid artery. Journal of Pharmacological Sciences 114(4): 390-398

Methylglyoxal (MGO), a metabolite of glucose, accumulates in vascular tissues of a hypertensive animal. In the present study, we examined the effect of MGO on angiotensin (Ang) II-induced contraction of rat carotid artery. Treatment of carotid artery with MGO (420 µM, 30 min) significantly augmented Ang II (0.1 to 30 nM)-induced concentration-dependent contraction. The effect was abolished by the removal of endothelium. BQ-123 (1, 5 µM), an endothelin A-receptor blocker, had no effect on the MGO-induced enhancement of Ang II-induced contraction. AL8810 (1 µM), a prostaglandin F(2α)-receptor blocker, or SQ29548 (1 µM), a thromboxane A(2)-receptor blocker, was also ineffective. However, tempol (10 µM), a superoxide scavenger, and catalase (5000 U/mL), which metabolizes hydrogen peroxide to water, significantly prevented the effect of MGO. Combined MGO and Ang II treatment increased reactive oxygen species (ROS) production. Apocynin (10 µM) or gp91ds-tat (3 µM), an inhibitor of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase, significantly prevented the effect of MGO. Gp91ds-tat or an Ang II type 1-receptor (AT1R) blocker, losartan (10 µM), prevented the MGO-mediated increased ROS production. The present study revealed that MGO augments Ang II-induced contraction by increasing AT1R-mediated NADPH oxidase-derived superoxide and hydrogen peroxide production in endothelium of rat carotid artery.


PMID: 21076237

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