geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

MicroRNA-338-3p promotes differentiation of mDPC6T into odontoblast-like cells by targeting Runx2


, : MicroRNA-338-3p promotes differentiation of mDPC6T into odontoblast-like cells by targeting Runx2. Molecular and Cellular Biochemistry 377(1-2): 143-149

Odontoblasts are terminally differentiated cells that play a vital role in dentinogenesis. The differentiation of odontoblasts is regulated by a variety of genetic and epigenetic mechanisms. Our previous microRNA microarray studies verified that miR-338-3p was up-regulated during odontoblast differentiation. The purpose of this study was to determine the function of miR-338-3p during odontoblast differentiation. The upregulation of miR-338-3p expression during odontoblast differentiation was validated by qRT-PCR. Odontoblast differentiation was enhanced after over-expression of miR-338-3p, while a loss of function approach using a miR-338-3p inhibitor impaired odontoblast differentiation. Bioinformatic analysis identified Runx2 as a potential target of miR-338-3p. Overexpression of miR-338-3p caused a decreased in the expression of Runx2 at both mRNA and protein levels, while Runx2 expression increased after treatment with miR-338-3p inhibitors. Furthermore, the activity of a luciferase reporter plasmid containing the 3'-UTR of Runx2 was significantly suppressed by ectopic expression of miR-338-3p. These results suggested that miR-338-3p promotes odontoblast differentiation through targeting Runx2.

US$19.90

PMID: 23380982

DOI: 10.1007/s11010-013-1580-3


Other references

Tamura, M., 1975: Invasion behaviour of nymph of. Journal of the Food Hygienic Society of Japan: 16 (special issue) 48-53

Picard L., 1939: On the structure of the Arabian peninsula. The principal tectonic processes which governed the formation of the structure of the Arabian peninsula were epeirogenic vertical movements, tangential movements, and faulting.

Hou; Bae, 2000: Biohybrid artificial pancreas based on macrocapsule device. On the basis of an overview of the current methodologies used for biohybrid artificial pancreas (BAP) and a critical analysis of the problems in designing the BAP devices, especially macrocapsule systems, a new type of refillable BAP is proposed....

Ziegler, H.H.vemann, K., 1976: Where are production potentials in swine fattening plants?. Kooperation 10 (9) 422-425

Slavin, S., 1987: Total lymphoid irradiation. The delivery of ionizing radiation to lymph nodes, thymus and spleen while non-lymphoid organs are shielded has profoundly immunosuppressive effects that may have value in transplantation and the treatment of autoimmune disorders. Here Shimon Slav...

Dykman, R., A.; Gantt, W.H.rsley, 1997: Experimental psychogenic hypertension: Blood pressure changes conditioned to painful stimuli (Schizokinesis). 1. During habituation in 4 dogs to a new environment and attachment of apparatus, the blood pressure was at first high but fell from about 175 systolic the first day to about 135 on the ninth day. 2. In the first group of dogs used two years previ...

Knoblauch, C., 2008: Central Switzerland Internist Week. Praxis 97(9): 463-463

Redtenbacher, K., 1978: Endodontia or exodontia: multi-canal and atypical teeth. 2. Der Zahnarzt; Colloquium Med. Dent 22(6): 287-298

Bekemeier, B.; Grembowski, D.; Yang, Y.Ran.; Herting, J.R., 2012: Local public health delivery of maternal child health services: are specific activities associated with reductions in Black-White mortality disparities?. To identify which MCH services delivered by local health departments (LHD) appear associated with reducing differences in Black-White mortality. We used a time-trend design to investigate relationships between change in MCH activities provided by...

Delabre, M.; Bianchi, A.; Véron, M., 1973: A critical study of methods in numerical taxonomy. The classification of aquatic bacteria. Annales de Microbiologie 124a(4): 489-506