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Morphological adaptation with preserved proliferation/transporter content in the colon of patients with short bowel syndrome


, : Morphological adaptation with preserved proliferation/transporter content in the colon of patients with short bowel syndrome. American Journal of Physiology. Gastrointestinal and Liver Physiology 297(1): G116-G123

In short bowel syndrome (SBS), although a remaining colon improves patient outcome, there is no direct evidence of a mucosal colonic adaptation in humans. This prospective study evaluates morphology, proliferation status, and transporter expression level in the epithelium of the remaining colon of adult patients compared with controls. The targeted transporters were Na+/H+ exchangers (NHE2 and 3) and oligopeptide transporter (PepT1). Twelve adult patients with a jejuno-colonic anastomosis were studied at least 2 yr after the last surgery and compared with 11 healthy controls. The depth of crypts and number of epithelial cells per crypt were quantified. The proliferating and apoptotic cell contents were evaluated by revealing Ki67, PCNA, and caspase-3. NHE2, NHE3, PepT1 mRNAs, and PepT1 protein were quantified by quantitative RT-PCR and Western blot, respectively. In patients with SBS compared with controls, 1) hyperphagia and severe malabsorption were documented, 2) crypt depth and number of cells per crypt were 35% and 22% higher, respectively (P < 0.005), whereas the proliferation and apoptotic levels per crypt were unchanged, and 3) NHE2 mRNA was unmodified; NHE3 mRNA was downregulated near the anastomosis and unmodified distally, and PepT1 mRNA and protein were unmodified. We concluded that, in hyperphagic patients with SBS with severe malabsorption, adaptive colonic changes include an increased absorptive surface with an unchanged proliferative/apoptotic ratio and well-preserved absorptive NHE2, NHE3, and PepT1 transporters. This is the first study showing a controlled nonpharmacological hyperplasia in the colon of patients with SBS.

US$19.90

PMID: 19389806

DOI: 10.1152/ajpgi.90657.2008


Other references

Sadowski, J.; Kompanowska Jezierska, E.; Dobrowolski, L.; Walkowska, A.; Badzynska, B., 1997: Renal medullary ion content during spontaneous variations of medullary blood flow in the rat Data from simultaneous recording. Journal of Physiology (Cambridge) 505P: 111P-112P, Dec

Wetherbee, J.N.; Chapman, P.D.; Bach, S.M.; Troup, P.J., 1988: Sequential shocks are comparable to single shocks employing two current pathways for internal defibrillation in dogs. Sequential pulse defibrillation using two current pathways was compared with single shocks simultaneously utilizing both pathways in 16 dogs to assess the effects of temporal summation. A cardioverter-defibrillator catheter was positioned via the...

Liotti F.S.; Menghini A.R.; Guerrieri P.; Bianchi R., 1989: Effects of dmso on friend erythroleukemic cell proliferation and on the activity of enzymes involved in this process. Variation in catalase, glutathione peroxidase (GP) and adenylate cyclase (AC) activity in murine erythroleukemic (MEL) cells were studied during multiplication and dimethylsulfoxide (DMSO)-induced differentiation. The results demonstrated that, al...

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Karppinen, H.; Hanninen, H., 2000: Forest conservation and economic utilization: public attitudes in Finland. Attitudes of the Finnish public towards the economic utilization of forests and forest conservation are examined using interview data collected in 1994. Principal component and cluster analyses are used to discern between persons with flexible and...

Zhang, D.; Deng, C.; Yang, Y., 2011: Methyl 3-(4-chloro-phen-yl)-2-(1,3-dimethyl-2,5-dioxo-4-phenyl-imidazolidin-4-yl)-3-oxopropano-ate. The title compound, C(21)H(19)ClN(2)O(5), is a tetra-substituted hydantoin derivative which contains an imidazolidine-2,4-dione core. The dihedral angle between the aromatic rings is 64.53 (14)°. In the crystal, weak inter-molecular C-H⋯O hyd...

Bronisch, T.; Wittchen, H.U., 1992: Lifetime and 6-month prevalence of abuse and dependence of alcohol in the Munich Follow-up Study. This paper reports lifetime and 6-month prevalence rates of alcohol abuse and dependence in West Germany. Assessment instruments are a modified German version of the Diagnostic Interview Schedule (DIS), a fully standardized interview for the asses...

Nashef, S.A.; Talwalkar, N.G.; Jamieson, M.P., 1987: Aortic arch rupture in a child following minor blunt trauma. We report the case of a seven-year-old boy who sustained near-total transection of the aortic arch following apparently minor blunt injury. The radiological and operative findings and the successful outcome of urgent surgical repair support an agg...

Bini, R.; Chiappe, C.; Marmugi, E.; Pieraccini, D., 2006: The "non-nucleophilic" anion [Tf2N]- competes with the nucleophilic Br-: an unexpected trapping in the dediazoniation reaction in ionic liquids. Imidazolium ionic liquids containing [Tf2N]- anion are not as innocent as they are often considered; [Tf2N]- is more reactive than Br- in heterolytic dediazoniation reactions.