+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Multifunctional label-free electrochemical biosensor based on an integrated aptamer

, : Multifunctional label-free electrochemical biosensor based on an integrated aptamer. Analytical Chemistry 80(13): 5110-5117

Aptamers, which are in vitro selected functional oligonucleotides, have been employed to design novel biosensors (i.e., aptasensors) due to their inherent selectivity, affinity, and their multifarious advantages over traditional recognition elements. In this work, we reported a multifunctional reusable label-free electrochemical biosensor based on an integrated aptamer for parallel detection of adenosine triphosphate (ATP) and alpha-thrombin, by using electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). A Au electrode as the sensing surface was modified with a part DNA duplex which contained a 5'-thiolated partly complementary strand (PCS) and a mixed aptamer (MBA). The unimolecular MBA contained small-molecule ATP binding aptamer (ABA) and also protein alpha-thrombin binding aptamer (TBA). Thus, the aptasensor could be used for detection of ATP and alpha-thrombin both. The detection limit of ATP was 1 x 10(-8) M, and its detection range could extend up to 10(-4) M, whereas the detection limit of alpha-thrombin was 1 x 10(-11) M, and its detection range was from 1 x 10(-11) to 1 x 10(-7) M. Meanwhile, after detecting alpha-thrombin, the sensing interface could be used for ATP recognition as well. The aptasensor regeneration could be realized by rehybridizing of the MBA strand with the partly complementary strand immobilized on the Au surface after ATP detection or by treating with a large amount of ATP and then rehybridizing the MBA strand with the partly complementary strand immobilized on the Au surface after alpha-thrombin detection. The aptasensor fabricated exhibited several advantages such as label-free detection, high sensitivity, regeneration, and multifunctional recognition. It also showed the detectability in biological fluid. Therein it held promising potential for integration of the sensing ability such as the simultaneous detection for multianalysis in the future.


PMID: 18522435

DOI: 10.1021/ac800303c

Other references

Blanco Sequeiros, R.; Klemola, R.; Ojala, R.; Jyrkinen, L.; Lappi-Blanco, E.; Soini, Y.; Tervonen, O., 2002: MRI-guided trephine biopsy and fine-needle aspiration in the diagnosis of bone lesions in low-field (0.23 T) MRI system using optical instrument tracking. The purpose of this study was to evaluate the feasibility of MRI-guided bone biopsy with optical instrument tracking and evaluate advantage of combined fine-needle aspiration (FNA) with trephine biopsy. Twenty trephine bone biopsies and 13 FNAs we...

Zuccarelli, M.T.; Jana, W.; Hourton, B.; Schmidt Hebbel, H., 1984: Bromatological study of two kinds of fat-coated biscuits. Several batches of 2 types of biscuit with chocolate-flavoured coating and containing milk, both popular with adults and children and eaten as snacks or instead of a bread portion at tea-time were analysed. The biscuits, of mean weight 26.3 and 30...

Jünger, M.; Hahn, M.; Klyscz, T.; Steins, A., 1999: Microangiopathy in the pathogenesis of chronic venous insufficiency. Current Problems in Dermatology 27: 124-129

Sprogøe-Jakobsen, U.; Karkov, J.N., 1991: Benign pancreatic glucagonoma and recurrent pulmonary embolism with fatal course in a 36-year old man. A case of glucagonoma of the pancreas, without necrolytic migratory erythema, but accompanied by lethal pulmonary embolism in a 36 year old man is presented. The pathology, epidemiology and treatment of the glucagonoma syndrome is briefly describe...

Szymczax, J.; Zechalko, A., 1963: Estimation of minute amounts of arsenic in foods and commonly used objects.. After reduction to arsine with Zn, As was estimatec colorimetrically as a complex with silver diethyl dithiocarbamate in pyridine solution. Ashing was no always necessary. The method was suitable for sample with 0.1 to 5 micro g As; with 1 micro g...

Timmel, H.; Zschiesche, W., 1959: Proposal for evaluation of a diapositive collection by means of punched cards. Zentralblatt für Allgemeine Pathologie U. Pathologische Anatomie 100: 19-23

Burt, C.T.; Roberts, J.; Lojeski, E.; Hitzig, B.M., 1986: Phosphorus and fluorine nmr examination of the anesthetized newt notopthalamus viridescens. We have examined newts by 19F-NMR using the anesthetic halothane as a probe and in another set of experiments taken 31P-NMR spectra under similar conditions. The spectra were recorded from the animal's tail. The water soluble 31P-NMR signals...

Sakata, T.; Miyahara, Y., 2005: Potentiometric Detection of Single Nucleotide Polymorphism by Using a Genetic Field-effect transistor. Potentiometric measurement of allele-specific oligonucleotide hybridization based on the principle of detection of charge-density change at the surface of a gate insulator by using of a genetic field-effect transistor has been demonstrated. Since...

Stone, A.A.; Donald Reed, R.; Schwartz, L., 1942: A new method for viewing sheet Kodachrome. Exposures are made under standard conditions, reduced if necessary and rephoto-graphed. The advantages are freedom from screen or grain and good rendering of skin tones and detail.

Jung, I.Lae.; Lee, J.Hye.; Kang, S.Chan., 2015: A potential oral anticancer drug candidate, iMoringa oleifera leaf extract, induces the apoptosis of human hepatocellular carcinoma cells. NlmCategory="UNASSIGNED">It has previously been reported that cold water-extracts of <i>Moringa oleifera</i> leaf have anticancer activity against various human cancer cell lines, including non-small cell lung cancer. In t...