geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Mutations that disrupt either the pqq or the gdh gene of Rahnella aquatilis abolish the production of an antibacterial substance and result in reduced biological control of grapevine crown gall


, : Mutations that disrupt either the pqq or the gdh gene of Rahnella aquatilis abolish the production of an antibacterial substance and result in reduced biological control of grapevine crown gall. Applied and Environmental Microbiology 75(21): 6792-6803

Rahnella aquatilis HX2, a biocontrol agent for grapevine crown gall caused by Agrobacterium vitis, produces an antibacterial substance that inhibits the growth of A. vitis in vitro. In this study, we show that MH15 and MH16, two Tn5-induced mutants of HX2, have lost their abilities to inhibit A. vitis and have reduced biocontrol activities; they grow in logarithmic phase at a rate similar to that of the wild type and have single Tn5 insertions. They are also impaired in producing pyrroloquinoline quinone (PQQ) or glucose dehydrogenase (GDH). Complementation of MH15 and MH16 with cosmid clones of CP465 and CP104 from an HX2 DNA library restored the antibiosis, biocontrol, and PQQ or GDH production phenotypes. A 6.7-kb BamHI fragment from CP465 that fully restored the MH15-affected phenotypes was cloned and sequenced. Sequence analysis of the mutated DNA region resulted in the identification of seven open reading frames (ORFs), six of which share significant homology with PQQ-synthesizing genes in other bacteria, designated pqqA through pqqF. Meanwhile, A 5.5-kb PstI fragment from CP104 fully complemented the MH16 mutant and contained a single ORF highly similar to that of genes coding for GDHs. An in-frame gdh deletion mutant has the same phenotypes as the Tn5 mutant of MH16. Complementation of both deletion and Tn5 gdh mutants restored the affected phenotypes to wild-type levels. Our results suggest that an antibacterial substance plays a role in biocontrol of A. vitis by HX2.

US$19.90

PMID: 19734331

DOI: 10.1128/AEM.00902-09


Other references

Guenette, S.; Pitcher, T.J., 1999: An age-structured model showing the benefits of marine reserves in controlling overexploitation. Previous modelling of areas closed to fishing (marine reserves) has generally employed non-dynamic models and has not included biological factors such as stock-recruitment and weight-fecundity relations. These models predicted that a marine reserv...

Malcolm, A.S.; Dexter, A.F.; Middelberg, A.P.J., 2006: Mechanical properties of interfacial films formed by lysozyme self-assembly at the air-water interface. We present the first characterization of the mechanical properties of lysozyme films formed by self-assembly at the air-water interface using the Cambridge interfacial tensiometer (CIT), an apparatus capable of subjecting protein films to a much h...

Molina, R.; Mateos, J.; Katsaggelos, A.K., 2006: Blind deconvolution using a variational approach to parameter, image, and blur estimation. Following the hierarchical Bayesian framework for blind deconvolution problems, in this paper, we propose the use of simultaneous autoregressions as prior distributions for both the image and blur, and gamma distributions for the unknown parameter...

Alvarez, W.C., 1974: Judging malignancy of colonic polyps. Geriatrics 29(1): 132-132

Oakes, H.; E.A., 1959: Soil survey of Creek County Oklahoma. U S Dept Agric Soil Conserv Serv Soil Surv 1950(5): 1-43

Stubblefield, P.G., 1977: Vaginal silastic device containing 0.25 percent 15s 15 methyl prostaglandin f 2 alpha methyl ester for early 2nd trimester abortion. Nine women thought to be 15-16 wk pregnant were treated with a vaginally inserted silastic device containing a 0.25% concentration of 15(S)-15 methyl prostaglandin F2.alpha. methyl ester for elective termination of pregnancy. All aborted before 22...

Garrison, DL.; Buck, KR.; Silver, MW., 1984: Microheterotrophs in the ice-edge zone. Antarctic Journal of the United States, 195: 109-111

Collette, JD.; Millam, JR.; Klasing, KC.; Wakenell, PS., 1999: Can early handling improve life in captivity?. Exotic Bird Report. June; 111: 1-2, 8

Zboralske F.F., 1970: Esophageal motion associated with peristalsis. Investigative Radiology: 273

Ishiguro, N.; Sato, G., 1988: Nucleotide sequence of insertion sequence IS3411, which flanks the citrate utilization determinant of transposon Tn3411. The nucleotide sequences of insertion sequences IS3411L (left) and IS3411R (right), present as direct terminal repeats in the citrate utilization of citrate utilization transposon Tn3411, and of IS3411 (generated by intramolecular recombination be...