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Nanoparticle-based immunochromatographic test strip with fluorescent detector for quantification of phosphorylated acetylcholinesterase: an exposure biomarker of organophosphorus agents

, : Nanoparticle-based immunochromatographic test strip with fluorescent detector for quantification of phosphorylated acetylcholinesterase: an exposure biomarker of organophosphorus agents. Analyst 138(18): 5431-5436

A nanoparticle-based fluorescence immunochromatographic test strip (FITS) coupled with a hand-held detector for highly selective and sensitive detection of phosphorylated acetylcholinesterase (AChE), an exposure biomarker of organophosphate (OP) pesticides and nerve agents, is reported. In this approach, OP-AChE adducts were selectively captured by quantum dot-tagged anti-AChE antibodies (Qdot-anti-AChE) and zirconia nanoparticles (ZrO2 NPs). The sandwich-like immunoreactions were performed among the Qdot-anti-AChE, OP-AChE and ZrO2 NPs to form a Qdot-anti-AChE-OP-AChE-ZrO2 complex, which was detected by recording the fluorescence intensity of Qdots captured during the test line. Paraoxon was used as the model OP pesticide. Under optimal conditions, this portable FITS immunosensor demonstrates a highly linear absorption response over the range of 0.01 nM to 10 nM OP-AChE, with a detection limit of 4 pM, coupled with good reproducibility. Moreover, the FITS immunosensor has been validated with OP-AChE spiked human plasma samples. This is the first report on the development of ZrO2 NP-based FITS for the detection of the OP-AChE adduct. The FITS immunosensor provides a sensitive and low-cost sensing platform for on-site screening/evaluating OP pesticides and nerve agents poisoning.


PMID: 23885349

DOI: 10.1039/c3an00621b

Other references

Sumner D.R., 1987: Efficacy of pencycuron against isolates representing different anastomosis groups of rhizoctonia solani and rhizoctonia like binucleate fungi. Pencycuron was evaluated for efficacy against isolates of Rhizoctonia solani and Rhizoctonia-like binucleate fungi in soil in the greenhouse at 10.0, 1.0, and 0.1 .mu.g/g of soil. Soil was infested separately with isolates of R. solani (AG-2 type...

Makhoul, F.; Kaur, P.; Johnston, T.D.; Jeon, H.; Gedaly, R.; Ranjan, D., 2008: Arteriovenous malformation of the pancreas: A case report and review of literature. Extrahepatic arteriovenous malformations (AVMs) of the gastrointestinal (GI) tract are rare and mostly asymptomatic congenital anomalies. The present case describes a 45-year-old woman with an AVM in the head of the pancreas, which caused massive...

Ju, W-Zheng.; Zhao, Y.; Liu, F.; Wu, T.; Zhang, J.; Liu, S-Jia.; Zhou, L.; Dai, G-Liang.; Xiong, N-Ning.; Fang, Z-Yuan., 2015: Clinical tolerability and pharmacokinetics of Erigerontis hydroxybenzene injection: results of a randomized phase I study in healthy Chinese volunteers. Multiple phenolic compounds in the extract of Erigeron breviscapus synergistically contribute to the neurovascular protective effects. We conducted a phase I and pharmacokinetic study with the phenolic compound-enriched product extracted from Erig...

Blanquet, P.; Meuniel, G.; Croizet, M.;, M., 1962: About the effect of potassium thlocyanate on the thyroidian metabolism in the rat. Once the hormonal molecules are synthetized the potassium thiocyanate is without action on the mechanism of liberation of thyroid hormones.

Altintas, M.A.; Vogt, P.M., 2014: Postbariatric plastic surgery. Postbariatric plastic surgery after major weight loss can improve quality of life through functional reconstruction and restoration of a normal body contour. Comorbidities associated with obesity necessitate individual indications and precise preo...

Renaud, R.; Lellouche, D., 1987: Oral estroprogestational contraception in 1987 and its complications. La Revue du Praticien 37(38): 2267-2276

Walker-Engström, M-Louise.; Ringqvist, I.; Vestling, O.; Wilhelmsson, B.; Tegelberg, A., 2003: A prospective randomized study comparing two different degrees of mandibular advancement with a dental appliance in treatment of severe obstructive sleep apnea. The objective of this study was to compare the effect of two different degrees of mandibular advancement (MA), 75% versus 50%, on somnographic variables after 6 months of dental appliance treatment in patients with severe obstructive sleep apnea (...

Lazo-Langner, A.; Kovacs, M.J., 2006: Standard-intensity vs low-dose anticoagulation for long-term management of patients with venous thromboembolism. Clinical Advances in Hematology & Oncology 3(11): 877-879

Bilz, R., 1964: Anthropological Analysis Of An Alcholic Hallucinosis. Discussions On Human Intelligence. Der Nervenarzt 35: 11-20

Hoenicka, M.; Schmid, C., 2008: Cardiovascular effects of modulators of soluble guanylyl cyclase activity. Soluble guanylyl cyclase (sGC) is one of the key enzymes of the nitric-oxide (NO)/cyclic 3',5'-guanosine monophosphate (cGMP) pathway. Located in virtually all mammalian cells, it controls the vessel tone, smooth muscle cell growth, plat...