+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Nanoscale organization of the pathogen receptor DC-SIGN mapped by single-molecule high-resolution fluorescence microscopy

, : Nanoscale organization of the pathogen receptor DC-SIGN mapped by single-molecule high-resolution fluorescence microscopy. Chemphyschem 8(10): 1473-1480

DC-SIGN, a C-type lectin exclusively expressed on dendritic cells (DCs), plays an important role in pathogen recognition by binding with high affinity to a large variety of microorganisms. Recent experimental evidence points to a direct relation between the function of DC-SIGN as a viral receptor and its spatial arrangement on the plasma membrane. We have investigated the nanoscale organization of fluorescently labeled DC-SIGN on intact isolated DCs by means of near-field scanning optical microscopy (NSOM) combined with single-molecule detection. Fluorescence spots of different intensity and size have been directly visualized by optical means with a spatial resolution of less than 100 nm. Intensity- and size-distribution histograms of the DC-SIGN fluorescent spots confirm that approximately 80 % of the receptors are organized in nanosized domains randomly distributed on the cell membrane. Intensity-size correlation analysis revealed remarkable heterogeneity in the molecular packing density of the domains. Furthermore, we have mapped the intermolecular organization within a dense cluster by means of sequential NSOM imaging combined with discrete single-molecule photobleaching. In this way we have determined the spatial coordinates of 13 different individual dyes, with a localization accuracy of 6 nm. Our experimental observations are all consistent with an arrangement of DC-SIGN designed to maximize its chances of binding to a wide range of microorganisms. Our data also illustrate the potential of NSOM as an ultrasensitive, high-resolution technique to probe nanometer-scale organization of molecules on the cell membrane.


PMID: 17577901

DOI: 10.1002/cphc.200700169

Other references

Anonymous, 1955: Twenty Five Years Ago. Indian Medical Gazette 90(6): 272-274

Yamada, H., 1977: Factor analysis of human teeth, dental arch and palate. Measurements of teeth, the dental arch and the palate in Japanese males were obtained from plaster casts of permanent dentition. Correlation coefficients and factor analysis of these 3 subjects were applied to declare what kinds of relation were i...

Mundu, M.M.; Chakravarty, A.K.; Chatterjee, S.K., 1963: Outbreak of hog cholera in Bihar. An outbreak with high mortality rate is described; the virus isolated was serologically identical to the European strain of swine fever virus and piglets experimentally infected with the local virus were protected by crystal violet vaccine obtaine...

Schlumpf, U., 1991: Application and value of nonsteroidal inflammation inhibitors in arthritis. The inflammatory stage of osteoarthritis when secondary synovitis contributes to the characteristic clinical presentation is first explained. During episodes of acute synovitis nonsteroidal antiinflammatory drugs may be particularly beneficial. Gu...

Shilts, M.Kay.; Horowitz, M.; Townsend, M.S., 2004: Goal setting as a strategy for dietary and physical activity behavior change: a review of the literature. Objective: To estimate the effectiveness of goal setting for nutrition and physical activity behavioural change, review the effect of goal-setting characteristics on behavioural change, and investigate the effectiveness of interventions containing...

Govind, M.; Shiney, K.; Chandrabhushan, S., 2016: A case report of kartagener syndrome. Journal of the Association of Physicians of India 64(1): 144-144

Gerasimene, G.B.; Kulene, V.V.; Glemzha, A.A., 1976: Phospho lipase ec of bacillus cereus. Phospholipase EC activity of 10 strains of B. cereus was studied. The most active phospholipase producing strain was selected. A cultivation mixture optimal for the phospholipase synthesis included peptone, yeast extract, glucose, NaCl and...

Guggenheim, K.G.; Butler, J.D.; Painter, P.P.; Lorsbach, B.A.; Tantillo, D.J.; Kurth, M.J., 2011: Synthesis of spiro-fused pyrazolidoylisoxazolines. Routes to structurally unique spiro-fused pyrazolidoylisoxazolines are reported. These methods start with monosubstituted hydrazines or hydrazides and utilize the nitrile oxide 1,3-dipolar cycloaddition reaction to generate the targeted spiro-fuse...

Wang, D.C.; Shannon, P.; Toi, A.; Chitayat, D.; Mohan, U.; Barkova, E.; Keating, S.; Tomlinson, G.; Glanc, P., 2015: Temporal lobe dysplasia: a characteristic sonographic finding in thanatophoric dysplasia. To determine the incidence of temporal lobe dysplasia (TLD) detected on prenatal ultrasound in thanatophoric dysplasia (TD) over an 11-year period in a tertiary referral center. An 11-year retrospective review of perinatal autopsies from 2002 to 2...

Fritsche, E., 1944: Quelques adapta-tions dos pattes chez les insectes. Naturalistes Belges, 25: 97-102, 122-128