geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Nerve growth factor mediated SH2-Bbeta/Akt signal pathway activated in allergic airway challenge in mice


, : Nerve growth factor mediated SH2-Bbeta/Akt signal pathway activated in allergic airway challenge in mice. Respirology 15(1): 80-87

Nerve growth factor (NGF) contributes to airway inflammation and bronchoconstriction in allergic asthma. The Src homology 2beta/serine/threonine kinase (SH2-Bbeta/Akt) pathway is one of the avenues through which NGF regulates the biological activity of pheochromocytoma (PC)12 cells. It has also been reported that NGF upregulates the expression of SH2-Bbeta in the lung tissue of asthmatic mice. The present study investigated the effects of NGF and SH2-Bbeta on Akt activation during allergic airway challenge. BALB/c mice were sensitized and challenged with ovalbumin. The effects of NGF and SH2-Bbeta on Akt in allergic airway challenge were assessed by intravenously administering anti-NGF antibody or a mutant of SH2-Bbeta (R555E) to these mice. Pulmonary histological changes were then assessed and the inflammatory cells in the BAL fluid (BALF) were counted. Additionally, phosphorylated Akt (p-Akt) expression was determined by fluorescence microscopy, western blotting and quantitative RT-PCR. Airway resistance was also measured using closed-type body plethysmography. We observed p-Akt overexpression in the lungs after allergen challenge by fluorescence microscopy, Western blotting and RT-PCR, as compared with the control. However, after treatment with anti-NGF or R555E, p-Akt levels and allergen-induced airway inflammation were reduced in comparison with those of allergen-challenged mice. Anti-NGF and R555E also decreased airway hyperresponsiveness caused by allergen challenge in response to methacholine (MCH). These results suggest that SH2-Bbeta regulation of Akt partly participates in the NGF-mediated development of allergic airway challenge.

US$19.90

PMID: 19947990

DOI: 10.1111/j.1440-1843.2009.01648.x


Other references

Komuro, M., 1976: Virus particles in ovarian tumors of ddy f mice exposed to x rays during early post natal life. Quantitative studies were performed on RNA tumor viruses in the irradiated ovaries of ddY/F and C3H/Tw strains of mice by EM. Four types of virus particles were identified: intracisternal A-(IAP), intracytoplasmic A-(CAP), B-particles and C-partic...

Fraioli, F.; Cappa, M.; Fabbri, A.; Gnessi, L.; Moretti, C.; Borrelli, P.; Isidori, A., 1984: Lack of endogenous opioid inhibitory tone on LH secretion in early puberty. Thirteen normal children, seven males and six females, during early puberty (I-II according to Tanner), have been studied. Each subject was injected at weekly intervals and in random order with 100 micrograms of LHRH, 0.2 mg/kg of naloxone and 0.9...

Friday A.E., 1978: Protein sequence data and the problem of minimum evolution. Biological Journal of the Linnean Society 10(4): 440-441

Andrew, N.; Rodgerson, L.; York, A., 2000: Frequent fuel-reduction burning: the role of logs and associated leaf litter in the conservation of ant biodiversity. Frequent low-intensity fires are used in management of Australian forests to reduce fuels loads and protect natural resources and human property. Low-intensity fires are typically patchy and unburned litter microhabitats are often associated with...

Lipstein B.; Hurwitz S.; Bornstein S., 1980: The nutritional value of algae for poultry dried chlorella in layer diets. The nutritional value of a sample of algae meal, mainly Chlorella, was assessed in an experiment with laying hens. Diets formulated by linear programming contained up to 120 g algae meal/kg with supplementary DL-Met. There were no significant effe...

Kreitz, C.; Kleiner, B.H., 1994: The monitoring and improvement of productivity in health care. Considers the monitoring of productivity in the health care environment. Examines especially the development of a labour standard and a volume indicator. Sees the whole as a management tool for controlling staffing requirements and costs. Investig...

Hvidt, V., 1962: Gestagen treatment of endometriosis of the colon. Report of a case treated with Enavid. The results are presented of a gestagen-induced pseudo-pregnancy in a 39-year-old woman suffering from stenosis of the pelvic colon due to endometriosis. Hormone therapy was instituted with the progestational synthetic steroid, norethynodrel, in c...

Hartman, L.J., 1966: Evolution of an effective municipal immunizing programme: an eight-year study. Medical Journal of Australia 2(20): 933-935

Cosenza, G.W.; Silva, T., 1972: Comparison of the speed with which African and Caucasian bees and their hybrids clean out combs. Selection for disease resistance is a decisive factor in competition between various races of honeybee. it was found that after 86 h, African colonies had removed 100% of (sealed) dead brood inserted in the hive, hybrids 99.21% and Caucasians 85.9...

Albarellos, G.A.; Montoya, L.; Quaine, P.C.; Landoni, M.F., 2011: Pharmacokinetics and bioavailability of a long-acting formulation of cephalexin after intramuscular administration to cats. The pharmacokinetic profile and bioavailability of a long-acting formulation of cephalexin after intramuscular administration to cats was investigated. Single intravenous (cephalexin lysine salt) and intramuscular (20% cephalexin monohydrate suspe...