geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Norepinephrine transport-mediated gene expression in noradrenergic neurogenesis


, : Norepinephrine transport-mediated gene expression in noradrenergic neurogenesis. Bmc Genomics 10(): 151-151

We have identified a differential gene expression profile in neural crest stem cells that is due to deletion of the norepinephrine transporter (NET) gene. NET is the target of psychotropic substances, such as tricyclic antidepressants and the drug of abuse, cocaine. NET mutations have been implicated in depression, anxiety, orthostatic intolerance and attention deficit hyperactivity disorder (ADHD). NET function in adult noradrenergic neurons of the peripheral and central nervous systems is to internalize norepinephrine from the synaptic cleft. By contrast, during embryogenesis norepinephrine (NE) transport promotes differentiation of neural crest stem cells and locus ceruleus progenitors into noradrenergic neurons, whereas NET inhibitors block noradrenergic differentiation. While the structure of NET und the regulation of NET function are well described, little is known about downstream target genes of norepinephrine (NE) transport. We have prepared gene expression profiles of in vitro differentiating wild type and norepinephrine transporter-deficient (NETKO) mouse neural crest cells using long serial analysis of gene expression (LongSAGE). Comparison analyses have identified a number of important differentially expressed genes, including genes relevant to neural crest formation, noradrenergic neuron differentiation and the phenotype of NETKO mice. Examples of differentially expressed genes that affect noradrenergic cell differentiation include genes in the bone morphogenetic protein (BMP) signaling pathway, the Phox2b binding partner Tlx2, the ubiquitin ligase Praja2, and the inhibitor of Notch signaling, Numbl. Differentially expressed genes that are likely to contribute to the NETKO phenotype include dopamine-beta-hydroxylase (Dbh), tyrosine hydroxylase (Th), the peptide transmitter 'cocaine and amphetamine regulated transcript' (Cart), and the serotonin receptor subunit Htr3a. Real-time PCR confirmed differential expression of key genes not only in neural crest cells, but also in the adult superior cervical ganglion and locus ceruleus. In addition to known genes we have identified novel differentially expressed genes and thus provide a valuable database for future studies. Loss of NET function during embryonic development in the mouse deregulates signaling pathways that are critically involved in neural crest formation and noradrenergic cell differentiation. The data further suggest deregulation of signaling pathways in the development and/or function of the NET-deficient peripheral, central and enteric nervous systems.

US$19.90

PMID: 19356247

DOI: 10.1186/1471-2164-10-151


Other references

Choi, K-Hee.; McFarland, W.; Neilands, T.B.; Nguyen, S.; Louie, B.; Secura, G.M.; Behel, S.; Mackellar, D.; Valleroy, L., 2004: An opportunity for prevention: prevalence, incidence, and sexual risk for HIV among young Asian and Pacific Islander men who have sex with men, San Francisco. Objectives: We investigated HIV prevalence and incidence and related risk factors among young Asian and Pacific Islander (API) men who have sex with men (MSM). Design: We conducted a cross-sectional survey. Methods: A venue-based sample of 496 you...

Park, S-Woo.; Seo, M-Seong., 2004: Subhyaloid hemorrhage treated with SF6 gas injection. A 72-year old woman with a premacular subhyaloid hemorrhage could not be treated by laser photodisruption due to a thick posterior cortical vitreous. Pure sulfur hexafluoride (SF6, 0.3 mL) gas was injected intravitreally without recombinant tissue...

Puljula, J.; Cygnel, H.; Mäkinen, E.; Tuomivaara, V.; Karttunen, V.; Karttunen, A.; Hillbom, M., 2013: Mild traumatic brain injury diagnosis frequently remains unrecorded in subjects with craniofacial fractures. Traumatic brain injuries (TBI) in subjects with craniofacial fractures are usually diagnosed by emergency room physicians. We investigated how often TBI remains unrecorded in these subjects, and whether diagnostic accuracy has improved after the i...

Cashman Richard E.; Leckie R.M.rk, 1993: Latitudinal variations of late Albian planktonic foraminiferal assemblages. Abstracts with Programs Geological Society of America: , Pages 430. 1993.

Ramesh Chand; Patil, B.P.; Ram Kishun, 1992: Efficacy of different chemicals against grapevine bacterial canker disease (Xanthomonas campestris pv. viticola). In field trials, efficacy of the treatments varied in 5 locations and from year to year (1989 and 1990). Copper oxychloride, followed by Bordeaux mixture, reduced disease intensity but were much less effective in areas with high and frequent rainf...

Costa, N.M. de S., 1984: Regions of production of forage plant seeds in Minas Gerais. Factors which are important in selecting regions for seed production of forage spp. including climate, rainfall, photoperiod, temp., radiation, RH, reproduction method and soil type are described. Data are given on many tropical grasses and legume...

Kolesnik, M.; Ferapontov, A., 1977: A machine for cleaning garlic. Konservnaia i ovoshchesushil'naia promyshlennost': 1 6-7

Filipovska, A.; Rackham, O., 2013: Specialization from synthesis: how ribosome diversity can customize protein function. It was thought that the proteins produced by ribosomes were dictated only by the sequences of the mRNAs they translated, however it is becoming apparent that subpopulations of ribosomes can have unique properties that influence the functions of th...

Morse, W.S., 1946: The use of fine cotton sutures in episiotomy repairs. Texas State Journal of Medicine 42: 282-282

Doose, G.; Haake, A.; Bernhart, S.H.; López, C.; Duggimpudi, S.; Wojciech, F.; Bergmann, A.K.; Borkhardt, A.; Burkhardt, B.; Claviez, A.; Dimitrova, L.; Haas, S.; Hoell, J.I.; Hummel, M.; Karsch, D.; Klapper, W.; Kleo, K.; Kretzmer, H.; Kreuz, M.; Küppers, R.; Lawerenz, C.; Lenze, D.; Loeffler, M.; Mantovani-Löffler, L.; Möller, P.; Ott, G.; Richter, J.; Rohde, M.; Rosenstiel, P.; Rosenwald, A.; Schilhabel, M.; Schneider, M.; Scholz, I.; Stilgenbauer, S.; Stunnenberg, H.G.; Szczepanowski, M., 2016: MINCR is a MYC-induced lncRNA able to modulate MYC's transcriptional network in Burkitt lymphoma cells. Despite the established role of the transcription factor MYC in cancer, little is known about the impact of a new class of transcriptional regulators, the long noncoding RNAs (lncRNAs), on MYC ability to influence the cellular transcriptome. Here,...