geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

On the reaction of D-amino acid oxidase with dioxygen: O2 diffusion pathways and enhancement of reactivity


, : On the reaction of D-amino acid oxidase with dioxygen: O2 diffusion pathways and enhancement of reactivity. Febs Journal 278(3): 482-492

Evidence is accumulating that oxygen access in proteins is guided and controlled. We also have recently described channels that might allow access of oxygen to pockets at the active site of the flavoprotein D-amino acid oxidase (DAAO) that have a high affinity for dioxygen and are in close proximity to the flavin. With the goal of enhancing the reactivity of DAAO with oxygen, we have performed site-saturation mutagenesis at three positions that flank the putative oxygen channels and high-affinity sites. The most interesting variants at positions 50, 201 and 225 were identified by a screening procedure at low oxygen concentration. The biochemical properties of these variants have been studied and compared with those of wild-type DAAO, with emphasis on the reactivity of the reduced enzyme species with dioxygen. The substitutions at positions 50 and 225 do not enhance this reaction, but mainly affect the protein conformation and stability. However, the T201L variant shows an up to a threefold increase in the rate constant for reaction of O(2) with reduced flavin, together with a fivefold decrease in the K(m) for dioxygen. This effect was not observed when a valine is located at position 201, and is thus attributed to a specific alteration in the micro-environment of one high-affinity site for dioxygen (site B) close to the flavin that plays an important role in the storage of oxygen. The increase in O(2) reactivity observed for T201L DAAO is of great interest for designing new flavoenzymes for biotechnological applications.

US$19.90

PMID: 21182588

DOI: 10.1111/j.1742-4658.2010.07969.x


Other references

Svoboda, M., 1988: Incidence of antibodies to Toxoplasma gondii in cats from Brno and the surrounding area. In the course of three years, 620 cats of all age categories were investigated for the occurrence of antibodies to Toxoplasma gondii. The serological examinations included the Sabin-Feldman reaction (SFR), complement-fixation reaction (CFR) and mi...

Janke, K., 1987: Die Makrofauna im Felswatt von Helgoland. Natur und Museum (Frankfurt am Main): 1173: 89-98

Yi, M.S.; Mrus, J.M.; Wade, T.J.; Ho, M.L.; Hornung, R.W.; Cotton, S.; Peterman, A.H.; Puchalski, C.M.; Tsevat, J., 2006: Religion, spirituality, and depressive symptoms in patients with HIV/AIDS. Depression has been linked to immune function and mortality in patients with chronic illnesses. Factors such as poorer spiritual well-being has been linked to increased risk for depression and other mood disorders in patients with HIV.We sought t...

Kessel David S.; Stein Joshua S., 2004: Overview of the performance assessment for the recertification of the Waste Isolation Pilot Plant. The Waste Isolation Pilot Plant (WIPP) is the only operational deep geologic repository in the world for transuranic nuclear waste. WIPP opened for disposal activities in March 1999, starting the clock for the periodic recertification required eve...

Jaishree, T.N.; Golden, B.L.; Hoffman, D.W.; Ramakrishnan, V.; White, S.W., 1995: Solution structure of ribosomal protein S17 by high resolution NMR spectroscopy. Journal of Cellular Biochemistry Supplement 0(21B): 59

Kerkeb, L.; Connolly, E.L., 2005: Iron transport and metabolism in plants. Genetic Engineering 27: 119-140

Isilak, Z.; Yalcın, M.; Un, H.; Kardesoglu, E., 2016: Fractional Flow Reserve-Guided Lesion or Patient Management?. Chinese Medical Journal 128(23): 3266-3266

Kapranov, S.V.; Sapel'nikov, A.Ia.; Sapel'nikova, L.Ia., 2015: Influence of the technogenic environment on health new-borns infant children in Lugansk region. The aim of the work was to evaluate the health of new-borns born to mothers who lived constantly influenced of the technogenic environmentat on health infant children in Luhansk region. We exameded evaluation of the influence of the technogenic en...

Polevoi, A.N., 1996: Simulation of yield formation by farm crops in conditions of anthropogenously polluted agroecosystems. The structure of a model has been developed, which described the effect of environmental factors and plant diseases on the quantity, quality and anthropogenously polluted agroecosystems, the grain crops serving as example. The effect of key enviro...

Bartalena, L., 2010: What to do for moderate-to-severe and active Graves' orbitopathy if glucocorticoids fail?. Treatment of Graves' orbitopathy (GO) is a major challenge, and the outcome of medical therapy is unsatisfactory in about one-third of cases. Glucocorticoids are the first-line therapy for moderate-to-severe and active GO, more commonly given...