+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

One-step purification and characterization of a beta-1,4-glucosidase from a newly isolated strain of Stereum hirsutum

, : One-step purification and characterization of a beta-1,4-glucosidase from a newly isolated strain of Stereum hirsutum. Applied Microbiology and Biotechnology 87(6): 2107-2116

A highly efficient beta-1,4-glucosidase (BGL) secreting strain, Stereum hirsutum SKU512, was isolated and identified based on morphological features and sequence analysis of internal transcribed spacer rDNA. A BGL containing a carbohydrate moiety was purified to homogeneity from S. hirsutum culture supernatants using only a single chromatography step on a gel filtration column. The relative molecular weight of S. hirsutum BGL was determined as 98 kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis or 780 kDa by size exclusion chromatography, indicating that the enzyme is an octamer. S. hirsutum BGL showed the highest activity toward p-nitrophenyl-beta-D-glucopyranoside (V (max) = 3,028 U mg-protein(-1), k (cat) = 4,945 s(-1)) ever reported. The enzyme also showed good stability at an acidic pH ranging from 3.0 to 5.5. The BGL was able to promote transglycosylation with an activity of 42.9 U mg-protein(-1) using methanol as an acceptor and glucose as a donor. The internal amino acid sequences of the isolated enzyme showed significant homology with hydrolases from glycoside hydrolase family 1 (GH1), indicating that the S. hirsutum BGL is a member of GH1 family. The characteristics of S. hirsutum BGL could prove to be of interest in several potential applications, especially in enhancing flavor release during the wine fermentation process.


PMID: 20532763

DOI: 10.1007/s00253-010-2668-2

Other references

Zerbe R.L., 1985: Genetic factors in normal and abnormal regulation of vasopressin secretion. Schrier, R W (Ed ) Vasopressin Xxiii+577p Raven Press: New York, N Y , Usa Illus 213-220

Dalecky, A.; Debout, G.; Mondor, G.; Rasplus, J.Y.; Estoup, A., 2002: PCR primers for polymorphic microsatellite loci in the facultatively polygynous plant-ant Petalomyrmex phylax (Formicidae). Fourteen microsatellite loci were isolated and their level of polymorphism characterized in two populations of the facultatively polygynous plant-ant Petalomyrmex phylax (Formicinae). High levels of within-population variation were observed at mos...

Feit, H.; Tindall, R.S.; Glasberg, M., 1982: Sources of error in the diagnosis of Guillain-Barre syndrome. A careful monitoring of the accuracy of diagnosis in six cases of Guillain-Barre syndrome has shown that a substantial proportion of these patients initially diagnosed as having Guillain-Barre syndrome on the basis of characteristic clinical findi...

Dearman, A.M.; Hise, E.F.; Bidlack, J.E.; Elmendorf, D.L., 2000: Humic acid as a soil amendment and its effects on soil microbial populations. Abstracts of the General Meeting of the American Society for Microbiology 100: 487

Evans, S.; Fishwick, C.; Sanger, K.; Fotherby, M.D., 1999: The effect of 6 months weight loss versus Bendrofluazide on 24-hour blood pressure in older mild hypertensives. Age & Ageing 28(SUPPL 2): 66, Sept

Reed G.W.J.; Jovanovic S., 1971: Halogens, mercury and some other trace elements in Apollo 12 samples. Pages 177 1971

Anonymous, 1945: FREUD on contraception. Human Fertility 10: 78-78

Baron, J.; Holzman, G.B.; Schulkin, J., 1999: Attitudes of obstetricians and gynecologists toward hormone replacement therapy. To determine the attitudes of obstetricians and gynecologists toward hormone replacement therapy (HRT), and the beliefs and intuitions that affected those attitudes. A questionnaire was sent to 1,000 gynecologists in the United States; 328 replies...

Zwol, B.H. van, 1989: Electronic sorting in the potato processing industry. The need for sorting of tubers and partially-processed potato products to eliminate defective or inadequately-peeled samples is discussed. Sorting after peeling and after cutting into dice, chips, etc. is considered. Basic principles of electronic...

Tsilimparis, N.; Faber, E.; Zindler, K.; Mohammad, W.; Hanack, U.; Yousefi, S.; Rückert, R.I., 2013: Aneurysms of the deep femoral artery: a systematic review of literature. True aneurysms of the deep femoral artery (APFA) are rare and are usually presented as case reports. Recommendations for diagnostics and therapy of APFAs are based on low-level evidence only. The purpose of this paper was to summarise the existing...