+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Overexpression, crystallization, and preliminary X-ray crystallographic analysis of shikimate dehydrogenase from Thermotoga maritima

, : Overexpression, crystallization, and preliminary X-ray crystallographic analysis of shikimate dehydrogenase from Thermotoga maritima. Acta Crystallographica. Section F, Structural Biology and Crystallization Communications 67(Pt 7): 824-826

Shikimate dehydrogenase (SDH), which catalyses the NADPH-dependent reduction of 3-dehydroshikimate to shikimate in the shikimate pathway, is an attractive target for the development of herbicides and antimicrobial agents. Previous structural studies showed that SDH exists in two conformations, an open form and a closed form, and it is believed that the conformational state is crucial to understanding a catalytic mechanism. To facilitate further structural comparisons among SDHs, structural analysis of an SDH from Thermotoga maritima encoded by the Tm0346 gene has been initiated. SDH from T. maritima has been overexpressed in Escherichia coli and crystallized at 296 K using ammonium sulfate as a precipitant. Crystals of T. maritima SDH diffracted to 1.45 Å resolution and belonged to orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a=54.21, b=62.45 and c=68.68 Å. The asymmetric unit contains a monomer, with a corresponding VM of 2.01 Å3 Da(-1) and a solvent content of 38.9% by volume.


PMID: 21795804

DOI: 10.1107/S1744309111019877

Other references

Wenzel, G., 1978: The harp-seal controversy and the Inuit economy. Arctic, 311: 3-6

Galbraith, R.A.; Drummond, G.S.; Kappas, A., 1985: Sn-protoporphyrin suppresses chemically induced experimental hepatic porphyria. Potential clinical implications. The ability of Sn(tin)-protoporphyrin to inhibit the induction of hepatic delta-aminolevulinate (ALA) synthase by allylisopropyl acetamide (AIA) was examined in the adult rat. Doses of Sn-protoporphyrin of 1, 10, and 50 mumol/kg body wt resulted i...

Ruehle Edward, 1951: Rola geologii w drugiej wojnie swiatowej. Discusses the role of geology in World War II, with particular reference to contributions in combat terrain analysis.

Soor, B., 1976: Doctors and dietary matters. Results of an enquiry by interview. Doctors of different categories working in the Stockholm area or in Varmland and born in 1909 or later were asked about the extent to which they had access to information on diet from various sources and their opinion of that information, also of...

McConnell, J., 2016: Running Injuries: The Infrapatellar Fat Pad and Plica Injuries. When considering knee pain in runners, clinicians differentiate sources of symptoms and determine their cause. Knee problems arise when a runner increases the amount/frequency of the loading through the lower limb. The way the loading is distribut...

Nguyen, M.C.; Garcia, L.A., 2008: Recent advances in atherectomy and devices for treatment of infra-inguinal arterial occlusive disease. The prevalence of peripheral arterial disease (PAD) continues to rise in an ever ageing society and consumes a significant part of health resources. Percutaneous revascularization has revolutionized the treatment of lower extremity peripheral vasc...

Klinec Albin, 1971: Main tectonic elements of the eastern Veporides. Lower part of the section of the nappe involving Paleozoic rocks in the west Carpathians, distribution of faults.

Pearce, J.K.; Stenzel, G., 1972: Logging and pulpwood production. A book designed primarily as a textbook for forestry schools in the USA and as a manual for the US logging industry, but intended also for foresters and logging managers in developing countries who are interested in mechanizing their logging opera...

Kalucy, M.J.; Grunstein, R.; Lambert, T.; Glozier, N., 2013: Obstructive sleep apnoea and schizophrenia – A research agenda. Schizophrenia is associated with significantly increased physical morbidity and mortality particularly secondary to cardiometabolic disorders. In people with schizophrenia, rates of obesity and the metabolic syndrome are high compared to the gener...

Poppe T.T., 1986: Kidney diseases of teleosts. Norsk Veterinaertidsskrift 98(11): 817-821