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PCR-based assay to detect sheeppox virus in ocular, nasal, and rectal swabs from infected Moroccan sheep


, : PCR-based assay to detect sheeppox virus in ocular, nasal, and rectal swabs from infected Moroccan sheep. Journal of Virological Methods 204: 38-43

Sheeppox is now enzootic in Morocco. The development of a reliable method for rapid diagnosis of the disease is a central part of any control strategy. The aim of this study is to determine the diagnostic value of a variety of clinical samples such as ovine nasal, ocular or rectal swabs for the detection of sheeppox virus (SPPV) by qualitative conventional polymerase chain reaction (PCR), using a single pair of primers targeting the inverted terminal repeats of the SPPV InS-1 strain, a virulent field isolate. Swab and blood samples were collected from forty animals naturally infected with SPPV who had clinical signs of sheeppox. All animals tested PCR-positive for SPPV. Positive results were obtained infrequently with blood samples, whereas swab samples from at least two sites (nasal, ocular, rectal) were positive per evaluated animal. These results indicate that swab samples are suitable for quantitative molecular SPPV diagnosis. PCR product sequences obtained from all types of sheep samples proved to be identical to the corresponding regions of sheeppox virus strain Romania 65.

US$19.90

PMID: 24698762

DOI: 10.1016/j.jviromet.2014.03.019


Other references

Dawson, J.L., 1998: Are basic surgical trainees being 'short-changed'?. Annals of the Royal College of Surgeons of England 80(4 Suppl): 163-5; Discussion 166-8

Bartley, G.E.; Schmidhauser, T.J.; Yanofsky, C.; Scolnik, P.A., 1990: Carotenoid desaturases from Rhodobacter capsulatus and Neurospora crassa are structurally and functionally conserved and contain domains homologous to flavoprotein disulfide oxidoreductases. The characteristic red color of some photosynthetic bacteria and the orange color of Neurospora conidia is due to the presence of carotenoids, photoprotective pigments synthesized by plants, algae, bacteria, and fungi. Generally, carotenoids are t...

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Turner, E.L.; Li, F.; Gallis, J.A.; Prague, M.; Murray, D.M., 2017: Review of Recent Methodological Developments in Group-Randomized Trials: Part 1-Design. In 2004, Murray et al. reviewed methodological developments in the design and analysis of group-randomized trials (GRTs). We have highlighted the developments of the past 13 years in design with a companion article to focus on developments in anal...

Newing, G.G., 1971: Weed control in mature tea. Dalapon had replaced the fork for the control of couch (Digitaria spp.) by the end of the 1950s thus obviating root damage to tea. A broken down vegetative carpet of pruning trash 2-3 in deep deters weed seed germination. From 1962 on simazine has...

Watanabe, H.; Ohmori, O.; Abe, K., 1997: Recurrent brief depression in Prader-Willi syndrome: a case report. Prader-Willi syndrome is caused by a deletion of 15q11-13 or maternal disomy of chromosome 15. A female patient with Prader-Willi syndrome, who was 19 years of age at the first onset of her recurrent brief episodes, is described. The episodes show...

Van Bezooijen, R.L.; Van Der Wee-Pals, L.; Papapoulos, S.E.; Löwik, C.W.G.M., 2002: Interleukin 17 synergises with tumour necrosis factor alpha to induce cartilage destruction in vitro. Background: Interleukin 17 (IL17) is produced by activated T cells and has been implicated in the development of bone lesions and cartilage degradation in rheumatoid arthritis (RA). Objective: To determine whether IL17, alone or together with tumo...

Patel, B.H. {a}; 1 ; Reddy, T.S.; 1 Department of Zoology, S.P.P.I.stitute of Science, B.U.iversity, B. - 364 002, G., I., 1990: An addition to the araneid fauna Araneae Arachnida of India. Records of the Zoological Survey of India 87.1-4: 157-164

Read, AW., 1984: Treatment of dermal ulcers in salamanders. Rephiberary, 2-3 No. 84

Valenzuela, J.G.; Charlab, R.; Gonzalez, E.C.; D.M.randa Santos, I.K.F.; Marinotti, O.; Francischetti, I.M.B.; Ribeiro, J.M.C., 2002: The D7 family of salivary proteins in blood sucking diptera. The D7 subfamily of salivary proteins is widespread in blood sucking Diptera and belongs to the superfamily of pheromone/odourant binding proteins. Although D7 proteins are among the most abundant salivary proteins in adult female mosquitoes and s...