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PI3 kinase directly phosphorylates Akt1/2 at Ser473/474 in the insulin signal transduction pathway

, : PI3 kinase directly phosphorylates Akt1/2 at Ser473/474 in the insulin signal transduction pathway. Journal of Endocrinology 220(1): 49-59

Insulin stimulated translocation of the glucose transporter GLUT4 from the cytosol to the plasma membrane in a concentration (1  nM-1  μM)-dependent manner and increased glucose uptake in 3T3-L1 adipocytes. Insulin-induced GLUT4 translocation to the cell surface was prevented by the phosphoinositide 3 kinase (PI3K) inhibitor wortmannin, the 3-phosphoinositide-dependent protein kinase 1 (PDK1) inhibitor BX912 or the Akt1/2 inhibitor MK2206, and by knocking-down PI3K, PDK1 or Akt1/2. Insulin increased phosphorylation of Akt1/2 at Thr308/309 and Ser473/474, to activate Akt1/2, in the adipocytes. Insulin-induced phosphorylation of Akt1/2 was suppressed by wortmannin and knocking-down PI3K, while no significant inhibition of the phosphorylation was obtained with BX912 or knocking-down PDK1. In the cell-free Akt assay, PI3K phosphorylated Akt1 both at Thr308 and Ser473 and Akt2 at Ser474 alone. In contrast, PDK1 phosphorylates Akt1 at Thr308 and Akt2 at Thr309. The results of this study indicate that PI3K activates Akt1, independently of PDK1, and Akt2 by cooperating with PDK1 in the insulin signal transduction pathway linked to GLUT4 translocation.


PMID: 24169049

DOI: 10.1530/JOE-13-0172

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Osborn, B.R., 1993: Confidentiality--at any price?. Confidentiality is a well respected and integral part of medical and dental practice and not without good reason. So fundamental is it to medicine in all its aspects, and so entrenched in the attitudes of those of us in the health care field, that...

Fertman, G.; Rakhmanin, I.; Ivanushko, L., 1977: Use of acetylcellulose as an ionite in a water preparation during the production of non alcoholic beverages. Izvestiia vysshikh uchebnykh zavedenii Pishchevaia tekhnologiia: 41-44

Hovind, O.B., 1983: Quality assurance and training for quality control. Medical Laboratory Sciences 40(3): 275-277

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Simon R.P.; Naughton M.P.; Darragh T.M.; Bayne L.L., 1981: Postictal pulmonary edema increased lymph flow is due to transiently elevated hydrostatic pressure. American Heart Association Monograph (82): IV-53

Zweig, A.; Bass, A.; Adar, R., 1980: Modified umbilical vein grafts in a contaminated wound. Harefuah 98(2): 79-79

Cohen, M.M.chael; Shklar, G., 1964: Deformities of the craniofacial and dental complex in the H-l virus modified hamster. The H-l virus modified hamster has a small flat face, microcephalic head, protuding large smooth tongue, abnormalities of the incisor teeth consisting of either absence or excessive overgrowth in a coil fashion, etc. X-rays show a good interdigita...