+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

PPARgamma regulates trophoblast proliferation and promotes labyrinthine trilineage differentiation

, : PPARgamma regulates trophoblast proliferation and promotes labyrinthine trilineage differentiation. Plos One 4(11): E8055-E8055

Abnormal trophoblast differentiation and function is the basis of many placenta-based pregnancy disorders, including pre-eclampsia and fetal growth restriction. PPARgamma, a ligand-activated nuclear receptor, plays essential roles in placental development; null murine embryos die at midgestation due to abnormalities in all placental layers, in particular, small labyrinth and expanded giant cell layer. Previous studies have focused mostly on the role of PPARgamma in trophoblast invasion. Based on the previously reported role of PPARgamma in preadipocyte differentiation, we hypothesized that PPARgamma also plays a pivotal role in trophoblast differentiation. To test this hypothesis, we report derivation of wild-type and PPARgamma-null trophoblast stem (TS) cells. PPARgamma-null TS cells showed defects in both proliferation and differentiation, specifically into labyrinthine trophoblast. Detailed marker analysis and functional studies revealed reduced differentiation of all three labyrinthine lineages, and enhanced giant cell differentiation, particularly the invasive subtypes. In addition, rosiglitazone, a specific PPARgamma agonist, reduced giant cell differentiation, while inducing Gcm1, a key regulator in labyrinth. Finally, reintroducing PPARgamma into null TS cells, using an adenovirus, normalized invasion and partially reversed defective labyrinthine differentiation, as assessed both by morphology and marker analysis. In addition to regulating trophoblast invasion, PPARgamma plays a predominant role in differentiation of labyrinthine trophoblast lineages, which, along with fetal endothelium, form the vascular exchange interface with maternal blood. Elucidating cellular and molecular mechanisms mediating PPARgamma action will help determine if modulating PPARgamma activity, for which clinical pharmacologic agonists already exist, might modify the course of pregnancy disorders associated with placental dysfunction.


PMID: 19956639

DOI: 10.1371/journal.pone.0008055

Other references

Molchanov, Mv, 1980: Increasing the capacity of dispensaries for calves. Zhivotnovodstvo (3) 53-54

Reed, H.C.B., 1982: Artificial insemination. The number of 1st inseminations carried out with semen from bulls of dairy, dual-purpose and beef breeds was 1 160 175, 9078 and 615 261 resp. in 1981/82 vs. 1 156 515, 9655 and 648 231 in 1980/81. The breeds responsible for more than or equal to...

Katz, B.J.; Crum, A.V.; Digre, K.B.; Warner, J.E.A., 2014: Optic disc edema and optic nerve head drusen. Journal of Neuro-Ophthalmology 33(2): 204-205

Urdén, G.; Chmielewska, J.; Carlsson, T.; Wiman, B., 1987: Immunological relationship between plasminogen activator inhibitors from different sources. Polyclonal antibodies have been raised against the inhibitor moiety in the purified complex between tissue plasminogen activator and its fast inhibitor (PA-inhibitor) in human plasma/serum. A radioimmunoassay for quantitation of PA-inhibitor antig...

Robinson, D.G.; Hillmer, S., 1990: Coated pits. Types of membrane coating, particularly clathrin coats, are distinguished and methods of studying clathrin coated invaginations of the plasma membrane, i.e. coated pits, their occurrence in plant cells and their role in endocytosis are discussed....

Koenigshof, Peter, 2003: Conodont deformation patterns and textural alteration in Paleozoic conodonts: Examples from Germany and France. Conodont deformation and textural alteration patterns in Devonian and Carboniferous conodont collections as well as new material have been examined using data from several hundred conodont samples from the Rheinisches Schiefergebirge (Germany) and...

Markert B., 1987: The pattern of distribution of lanthanide elements in soils and plants. The concentration of lanthanides (rare earth elements) in plant[Vaccinium vitis-idaea; Ericaceae; red whortleberry] and soil samples were determined by means of atomic emission spectroscopy with inductively coupled plasma (AES-ICP). The abundance...

Eremin, E.M., 1971: Some aspects of calculating the size of mechanized logging gangs. The theory of 'mass servicing' is applied to calculation of optimum gang size in mechanized logging operations. From this, and from time-study observations on logging gangs of various sizes in different parts of the USSR, a table is draw...

Gázquez Evangelista, J.A.; Benavente García, J.L., 1983: Intentional drug poisoning as a parasuicidal behavior. Actas Luso-Espanolas de Neurologia, Psiquiatria Y Ciencias Afines 11(4): 313-320

Harmeyer J.; Varady J., 1973: Measurement of urea conversion in small ruminants with different feed and starvation. Zeitschrift fuer Tierphysiologie Tierernaehrung und Futtermittelkunde 32(1): 32-33