geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Peptides derived from Rhopilema esculentum hydrolysate exhibit angiotensin converting enzyme (ACE) inhibitory and antioxidant abilities


, : Peptides derived from Rhopilema esculentum hydrolysate exhibit angiotensin converting enzyme (ACE) inhibitory and antioxidant abilities. Molecules 19(9): 13587-13602

Jellyfish (Rhopilema esculentum) was hydrolyzed using alcalase, and two peptides with angiotensin-I-converting enzyme (ACE) inhibitory and antioxidant activities were purified by ultrafiltration and consecutive chromatographic methods. The amino acid sequences of the two peptides were identified as VKP (342 Da) and VKCFR (651 Da) by electrospray ionization tandem mass spectrometry. The IC50 values of ACE inhibitory activities of the two peptides were 1.3 μM and 34.5 μM, respectively. Molecular docking results suggested that VKP and VKCFR bind to ACE through coordinating with the active site Zn(II) atom. Free radical scavenging activity and protection against hydrogen peroxide (H2O2)-induced rat cerebral microvascular endothelial cell (RCMEC) injury were used to evaluate the antioxidant activities of the two peptides. As the results clearly showed that the peptides increased the superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-px) activities in RCMEC cells), it is proposed that the R. esculentum peptides exert significant antioxidant effects.

US$19.90

PMID: 25185066

DOI: 10.3390/molecules190913587


Other references

Kessler, H.; Müller, E., 1966: Separation of multinucleic aromatic compounds by thin layer chromatography using picric acids as complex forming agent. Journal of Chromatography 24(2): 469-473

Tani A.; Ishida Y.; Morozumi T.; Matsubara N.; Hori M.; Katabatake A.; Kamada T.; Kimura K.; Takijiri C.; Hshimoto K., 1991: Relation between myocardial fibrosis assessed with thallium 201 myocardial spect and extracardiac abnormalities in patients with systemic sclerosis. Japanese Circulation Journal 55(SUPPL A): 156

Kaufmann, P.; Tilz, G.P.; Smolle, K.H.; Demel, U.; Krejs, G.J., 1996: Increased plasma concentrations of circulating intercellular adhesion molecule-1 (cICAM-1) in patients with necrotizing pancreatitis. The intercellular adhesion molecule-1 (ICAM-1), a membrane glycoprotein, is important in the adhesion of cytokine-stimulated leukocytes to the endothelium of microvessels and their transendothelial migration. Circulating isoforms of ICAM-1 (cICAM-...

Lamb, C.R.; Gregory, S.P.; Brockman, D.; Mantis, P.S.; Dobson, J., 2003: Variations in the radiographic appearance of a nasal adenocarcinoma over time. A 9 year old, neutered male, mixed breed dog had epistaxis from the right nostril, right epiphora and marked upper respiratory noise. Initial radiographs revealed lesions typical of primary nasal neoplasia, but the biopsy was non-diagnostic. Subse...

Miyagi, T.; Hata, K.; Konno, K.; Tsuiki, S., 1992: Multiple forms of mammalian sialidase: altered expression in carcinogenesis. We have demonstrated that rat liver contains at least four types of sialidase differing in subcellular location, in catalytic property and immunologically. They are intralysosomal, cytosolic and membrane-associated sialidases I and II. Membrane si...

Lukaszewicz, B.; Krumpholc, B., 1972: Evaluation of retrobulbar injections of pridasol and vitamin B-12 in some eye diseases. Klinika Oczna 42(2): 739-741

Reiser, O., 1929: Die Bedeutung der Vogeleier fur die Systematik. Verhandlungen der Zoologisch-Botanischen Gesellschaft in Wien, 79 23-24

Makkaveev, B., 1961: A new method of controlling infertility and cows coming on heat again. At oestrus, smears of cervical mucus are characterized by a fern-like pattern. When signs of heat are not apparent smears should be examined and if the fern-like pattern is noted the should be inseminated if the cervix is open. A 2nd test should b...

Hahn R.A.; Macdonald B.R.; Martin M.A., 1983: Anti hypertensive activity of ly 141865 trans racemic 4 4a 5 6 7 8ag octa hydro 5 propyl 2h pyrazolo 3 4 g quinoline di hydro chloride a selective presynaptic dopamine receptor agonist. Oral (0.3-3 mg/kg) and i.v. (1-1000 .mu.g/kg) administration of LY14865 lowered mean arterial blood pressure and cardiac rate in anesthetized and conscious spontaneously hypertensive rats (SHR). These effects were dose-related, rapid in onset and...

Tsuji K.; Nakagawa Y., 1984: Effects of simultaneous feeding of konjac amorphophallus konjac mannan and cellulose on the growth organ weight and lipid levels in hyper cholesterolemic rats. The effect of combined feeding of 2 dietary fibers, cellulose and konjac flour, on the growth, organ weight and serum lipid levels of rats fed a high cholesterol diet was studied. Rats given a diet supplemented with 0.5% cholesterol and 0.25% sodi...