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Phosphorylation of beta-actin by protein kinase C-delta in camptothecin analog-induced leukemic cell apoptosis


, : Phosphorylation of beta-actin by protein kinase C-delta in camptothecin analog-induced leukemic cell apoptosis. Acta Pharmacologica Sinica 29(1): 135-142

This study was conducted to reveal new proteins involved in acute myeloid leukemia (AML) cell apoptosis. Using camptothecin analog NSC606985- induced leukemic U937 cell apoptosis as a model, this study performed a differential proteomic analysis during apoptosis induction. The significantly modulated protein was underwent further investigation in the apoptotic process. We found that beta-actin protein presented two different spots on the two-dimensional electrophoresis (2-DE) map, which shared similar molecular weight and different pI. Those two spots demonstrated contrary changes (disappeared on the basic-end and increased on the acid-end spot) during apoptosis induction, although the total level of beta-actin kept constant. This observation was further confirmed by immunoblot analysis on 2-DE gel. When NSC606985-treated cell lysate was incubated with alkaline phosphotase, beta-actin on the basic-end spot was restored, indicating increased phosphorylation of beta-actin during NSC606985- induced apoptosis. Moreover, the polymerization of actin also decreased after NSC606985 treatment. The increased beta-actin phosphorylation and decreased actin polymerization was antagonized by pre-treatment of rottlerin, a specific protein kinase C-delta (PKC delta) inhibitor. All these results indicate that beta-actin was phosphorylated during apoptosis induction, which was mediated by activated PKC delta.

US$19.90

PMID: 18158875

DOI: 10.1111/j.1745-7254.2008.00753.x


Other references

Van Den Berg E.; Meyer A.J.; Heyns J., 1986: Two new elaphonema species from the cape province south africa nematoda rhabditida. Two new species of the genus Elaphonema Heyns, 1962 are described and figured from South Africa. E. karooense sp. n. is characterized by a greater body length, a vulval area which stretches over 20 to 23 annules and spicules which appear to have h...

Degroote, C.W., 1983: Frontal open bite: incidence, etiology and cephalometric characteristics; a literature review. Acta Stomatologica Belgica 80(4): 271-283

Kent, S.J.; Fernandez, C.S.; Dale, C.Jane.; Davenport, M.P., 2005: Reversion of immune escape HIV variants upon transmission: insights into effective viral immunity. Many viruses that cause chronic viremic infections, such as human immunodeficiency virus type 1 (HIV-1), mutate extensively to avoid effective control by the host immune system. However, each immune escape mutation probably results in some fitness...

Persson, H.; Rythe'n Alder, E.; Melcher, A.; Erhardt, L., 1994: Metoprolol and xamoterol infarction study - MEXIS The results on exercise tolerance. European Heart Journal 15(ABSTR SUPPL ): 103

West, J.L., 1940: Coecidiosis of Domesticated Animals and Fowls. A brief review of the subject as applied to cattle, fur-bearing animals, and poultry. Life-cycles, occurrence, pathology, arid the questions of immunity, treatment, and preventive measures are dealt with in very general terms. Astringents are reco...

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Fasoli, A.; Romagnoli, P.; Botta, F.; Giannini, E.; Chiarbonello, B.; Malfatti, F.; Testa, R., 2001: Platelets/spleen area ratio reflects progressive liver disease. Journal of Hepatology 34(Supplement 1): 235, April

Li, M-Keng.; Li, Y-Jie.; Zhang, G-Feng.; Chen, J-Qi.; Zhang, J-Ping.; Qi, J.; Huang, Y.; Lai, X-Sheng.; Tang, C-Zhi., 2016: Acupuncture for ischemic stroke: cerebellar activation may be a central mechanism following Deqi. The needling sensation of Deqi during acupuncture is a key factor of influencing acupuncture outcome. Recent studies have mainly focused on the brain function effects of Deqi in a physiological state. Functional magnetic resonance imaging (fMRI) o...

Streel, S.; Donneau, A-F.; Hoge, A.; Albert, A.; Schoenen, J.; Guillaume, M., 2016: One-year prevalence of migraine using a validated extended French version of the ID Migraine™: A Belgian population-based study. Country-specific prevalence data on migraine and comorbidities are vital to assess the public health burden of migraine and the corresponding resources required for proper management. Considering the absence of reliable statistics, this study aime...

Wiken, T.; Richard, O., 1954: Physiology of the wine yeasts. V. Carbon dioxide production by Fendant wine yeasts in nitrogen or in argon, air and nitrogen or in mixture of argon and air. Antonie van Leeuwenhoek 20(4): 385-405